|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 276, Issue 21, 17985-17993, May 25, 2001
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
From the Department of Molecular Biology, University of Texas
Southwestern Medical Center, Dallas, Texas 75390-9148
In pancreatic acinar cells, the HOX-like factor
PDX1 acts as part of a trimeric complex with two TALE class homeodomain
factors, PBX1b and MEIS2b. The complex binds to overlapping half-sites for PDX1 and PBX. The trimeric complex activates transcription in cells
to a level about an order of magnitude greater than PDX1 alone. The
N-terminal PDX1 activation domain is required for detectable transcriptional activity of the complex, even though PDX1 truncations bearing only the PDX1 C-terminal homeodomain and pentapeptide motifs
can still participate in forming the trimeric complex. The conserved
N-terminal PBC-B domain of PBX, as well as its homeodomain, is required
for both complex formation and transcriptional activity. Only the
N-terminal region of MEIS2, including the conserved MEIS domains, is
required for formation of a trimer on DNA and transcriptional activity:
the MEIS homeodomain is dispensable. The activity of the
pancreas-specific ELA1 enhancer requires the cooperation of the trimer-binding element and a nearby element that binds the pancreatic transcription factor PTF1. We show that the
PDX1· PBX1b·MEIS2b complex cooperates with the PTF1 basic
helix-loop-helix complex to activate an ELA1 minienhancer
in HeLa cells and that this cooperation requires all three homeoprotein
subunits, including the PDX1 activation domain.
DNA Binding and Transcriptional Activation by a
PDX1·PBX1b·MEIS2b Trimer and Cooperation with a
Pancreas-specific Basic Helix-Loop-Helix Complex*
*
This research was supported by Grant DK-55266 from the
National Institutes of Health.The costs of publication of this
article were defrayed in part by the
payment of page charges. The article must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
To whom correspondence should be addressed: Dept. of Molecular
Biology, University of Texas Southwestern Medical Center, 5323 Harry
Hines Blvd., Dallas, TX 75390-9148. Tel.: 214-648-1942; Fax:
214-648-1915; E-mail: swift@hamon.swmed.edu.
Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  M.-J. Boucher, M. Simoneau, and H. Edlund The Homeodomain-Interacting Protein Kinase 2 Regulates Insulin Promoter Factor-1/Pancreatic Duodenal Homeobox-1 Transcriptional Activity Endocrinology, January 1, 2009; 150(1): 87 - 97. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. M. Oliver-Krasinski and D. A. Stoffers On the origin of the {beta} cell Genes & Dev., August 1, 2008; 22(15): 1998 - 2021. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Masui, Q. Long, T. M. Beres, M. A. Magnuson, and R. J. MacDonald Early pancreatic development requires the vertebrate Suppressor of Hairless (RBPJ) in the PTF1 bHLH complex Genes & Dev., October 15, 2007; 21(20): 2629 - 2643. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. B. Deramaudt, M. M. Sachdeva, M. P. Wescott, Y. Chen, D. A. Stoffers, and A. K. Rustgi The PDX1 Homeodomain Transcription Factor Negatively Regulates the Pancreatic Ductal Cell-specific Keratin 19 Promoter J. Biol. Chem., December 15, 2006; 281(50): 38385 - 38395. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. M. Beres, T. Masui, G. H. Swift, L. Shi, R. M. Henke, and R. J. MacDonald PTF1 Is an Organ-Specific and Notch-Independent Basic Helix-Loop-Helix Complex Containing the Mammalian Suppressor of Hairless (RBP-J) or Its Paralogue, RBP-L Mol. Cell. Biol., January 1, 2006; 26(1): 117 - 130. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.-H. Chao, J. N. Harada, F. Hyndman, X. Gao, C. G. Nelson, S. K. Chanda, and J. S. Caldwell PDX1, a Cellular Homeoprotein, Binds to and Regulates the Activity of Human Cytomegalovirus Immediate Early Promoter J. Biol. Chem., April 16, 2004; 279(16): 16111 - 16120. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Wang, G. Webb, Y. Cao, and D. F. Steiner Contrasting patterns of expression of transcription factors in pancreatic {alpha} and {beta} cells PNAS, October 28, 2003; 100(22): 12660 - 12665. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-S. Annicotte, E. Fayard, G. H. Swift, L. Selander, H. Edlund, T. Tanaka, T. Kodama, K. Schoonjans, and J. Auwerx Pancreatic-Duodenal Homeobox 1 Regulates Expression of Liver Receptor Homolog 1 during Pancreas Development Mol. Cell. Biol., October 1, 2003; 23(19): 6713 - 6724. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Kobayashi, M. Fujioka, E. N. Tolkunova, D. Deka, M. Abu-Shaar, R. S. Mann, and J. B. Jaynes Engrailed cooperates with extradenticle and homothorax to repress target genes in Drosophila Development, February 15, 2003; 130(4): 741 - 751. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. A. Gregory and P. I. Mackenzie The Homeodomain Pbx2-Prep1 Complex Modulates Hepatocyte Nuclear Factor 1alpha -Mediated Activation of the UDP-Glucuronosyltransferase 2B17 Gene Mol. Pharmacol., July 1, 2002; 62(1): 154 - 161. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |