JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M100565200 on March 13, 2001

J. Biol. Chem., Vol. 276, Issue 21, 18046-18051, May 25, 2001
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
276/21/18046    most recent
M100565200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Cavelier, L. B.
Right arrow Articles by Rubin, E. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Cavelier, L. B.
Right arrow Articles by Rubin, E. M.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Regulation and Activity of the Human ABCA1 Gene in Transgenic Mice*

Lucia B. CavelierDagger , Yang QiuDagger , John K. Bielicki, Veena Afzal, Jan-Fang Cheng§, and Edward M. Rubin§

From the Genome Sciences Department, Lawrence Berkeley National Laboratory, Berkeley, California 94720

The ABCA1 transporter is one of the limiting steps in cellular cholesterol efflux. To study the expression and activity of the human ABCA1 gene in vivo we have examined mice containing two human BAC transgenes with different 5' ends. Mice containing a 255-kilobase (kb) BAC transgene, including 70 kb upstream of the previously defined exon 1, demonstrated a pattern of tissue-specific expression mimicking that of the endogenous mouse gene. Compared with macrophages from control mice, macrophages from these transgenics had increases in apoA-I cholesterol efflux heightened in response to increases in cell cholesterol content. The observed increase in macrophage apoA-I-mediated cholesterol efflux was not accompanied by alterations in plasma high density lipoprotein in the transgenics. Although mice containing a smaller 171-kb human BAC transgene, lacking the previously described exon 1 and ABCA1 promoter, did not express human ABCA1 in macrophages, they did express the human transgene in liver at levels comparable with those of the orthologous mouse gene. Analysis by 5' rapid amplification of cDNA ends of liver mRNA from these animals revealed a new ABCA1 exon 1 (exon 1A) and a previously unrecognized promoter. Analysis of human tissue revealed that exon 1A containing transcripts accounted for a high proportion of the ABCA1 mRNAs present in human liver. This analysis of ABCA1 transgenics showed that the expression of human ABCA1 transgenes can result in increased cholesterol efflux from macrophages, unaccompanied by changes in plasma high density lipoprotein, and identified a new ABCA1 promoter in humans.

* This work was supported by NHLBI, National Institutes of Health, Programs for Genomic Applications Grant HL66728-01, the National Institutes of Health Grant HL63897-01, the Swedish Medical Research Council and the Knut and Alice Wallenberg Stiftelse for postdoctoral fellowships (to L. B. C.), and National Institutes of Health Grant HL59483-01A2 (to J. K. B.). Research was conducted at the E. O. Lawrence Berkeley National Laboratory and performed under Department of Energy Contract DE-AC0376SF00098, University of California.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Dagger Both authors contributed equally to this work.

§ To whom correspondence should be addressed: Genome Sciences Dept., Lawrence Berkeley National Laboratory, One Cyclotron Rd., MS-84-171 Berkeley, CA 94720. Tel.: 510-486-5072; Fax: 510-486-4229; E-mail: Emrubin@lbl.gov or JFCheng{at}lbl.gov.

Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
X. Zhou, W. He, Z. Huang, A. M. Gotto Jr., D. P. Hajjar, and J. Han
Genetic Deletion of Low Density Lipoprotein Receptor Impairs Sterol-induced Mouse Macrophage ABCA1 Expression: A NEW SREBP1-DEPENDENT MECHANISM
J. Biol. Chem., January 25, 2008; 283(4): 2129 - 2138.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
N. Tamehiro, Y. Shigemoto-Mogami, T. Kakeya, K.-i. Okuhira, K. Suzuki, R. Sato, T. Nagao, and T. Nishimaki-Mogami
Sterol Regulatory Element-binding Protein-2- and Liver X Receptor-driven Dual Promoter Regulation of Hepatic ABC Transporter A1 Gene Expression: MECHANISM UNDERLYING THE UNIQUE RESPONSE TO CELLULAR CHOLESTEROL STATUS
J. Biol. Chem., July 20, 2007; 282(29): 21090 - 21099.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
Y. Wang and J. F. Oram
Unsaturated fatty acids phosphorylate and destabilize ABCA1 through a protein kinase C {delta} pathway
J. Lipid Res., May 1, 2007; 48(5): 1062 - 1068.
[Abstract] [Full Text] [PDF]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
W. Le Goff, P. Zheng, G. Brubaker, and J. D. Smith
Identification of the cAMP-Responsive Enhancer of the Murine ABCA1 Gene: Requirement for CREB1 and STAT3/4 Elements
Arterioscler. Thromb. Vasc. Biol., March 1, 2006; 26(3): 527 - 533.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. Maric, R. S. Kiss, V. Franklin, and Y. L. Marcel
Intracellular Lipidation of Newly Synthesized Apolipoprotein A-I in Primary Murine Hepatocytes
J. Biol. Chem., December 2, 2005; 280(48): 39942 - 39949.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y. Wang and J. F. Oram
Unsaturated Fatty Acids Phosphorylate and Destabilize ABCA1 through a Phospholipase D2 Pathway
J. Biol. Chem., October 28, 2005; 280(43): 35896 - 35903.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
R. R. Singaraja, E. R. James, J. Crim, H. Visscher, A. Chatterjee, and M. R. Hayden
Alternate transcripts expressed in response to diet reflect tissue-specific regulation of ABCA1
J. Lipid Res., October 1, 2005; 46(10): 2061 - 2071.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
R. S. Kiss, J. Maric, and Y. L. Marcel
Lipid efflux in human and mouse macrophagic cells: evidence for differential regulation of phospholipid and cholesterol efflux
J. Lipid Res., September 1, 2005; 46(9): 1877 - 1887.
[Abstract] [Full Text] [PDF]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
S. Ragozin, A. Niemeier, A. Laatsch, B. Loeffler, M. Merkel, U. Beisiegel, and J. Heeren
Knockdown of Hepatic ABCA1 by RNA Interference Decreases Plasma HDL Cholesterol Levels and Influences Postprandial Lipemia in Mice
Arterioscler. Thromb. Vasc. Biol., July 1, 2005; 25(7): 1433 - 1438.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
H. Zheng, R. S. Kiss, V. Franklin, M.-D. Wang, B. Haidar, and Y. L. Marcel
ApoA-I Lipidation in Primary Mouse Hepatocytes: SEPARATE CONTROLS FOR PHOSPHOLIPID AND CHOLESTEROL TRANSFERS
J. Biol. Chem., June 3, 2005; 280(22): 21612 - 21621.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
J. M. Coutinho, R. R. Singaraja, M. Kang, D. J. Arenillas, L. N. Bertram, N. Bissada, B. Staels, J.-C. Fruchart, C. Fievet, A. M. Joseph-George, et al.
Complete functional rescue of the ABCA1-/- mouse by human BAC transgenesis
J. Lipid Res., June 1, 2005; 46(6): 1113 - 1123.
[Abstract] [Full Text] [PDF]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
J. Huuskonen, M. Vishnu, P. E. Fielding, and C. J. Fielding
Activation of ATP-Binding Cassette Transporter A1 Transcription by Chromatin Remodeling Complex
Arterioscler. Thromb. Vasc. Biol., June 1, 2005; 25(6): 1180 - 1185.
[Abstract] [Full Text] [PDF]

Home page
 Vasc MedHome page
S Soumian, C Albrecht, A. Davies, and R. Gibbs
ABCA1 and atherosclerosis
Vascular Medicine, May 1, 2005; 10(2): 109 - 119.
[Abstract] [PDF]

Home page
 J Am Coll CardiolHome page
A. M. Gotto Jr and E. A. Brinton
Assessing low levels of high-density lipoprotein cholesterol as a risk factor in coronary heart disease: A working group report and update
J. Am. Coll. Cardiol., March 3, 2004; 43(5): 717 - 724.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
P. Costet, F. Lalanne, M. C. Gerbod-Giannone, J. R. Molina, X. Fu, E. G. Lund, L. J. Gudas, and A. R. Tall
Retinoic Acid Receptor-Mediated Induction of ABCA1 in Macrophages
Mol. Cell. Biol., November 1, 2003; 23(21): 7756 - 7766.
[Abstract] [Full Text] [PDF]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
D. L. Macdonald, T. L. Terry, L. B. Agellon, P. N. Nation, and G. A. Francis
Administration of Tyrosyl Radical-Oxidized HDL Inhibits the Development of Atherosclerosis in Apolipoprotein E-Deficient Mice
Arterioscler. Thromb. Vasc. Biol., September 1, 2003; 23(9): 1583 - 1588.
[Abstract] [Full Text] [PDF]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
R. J. Aiello, D. Brees, and O. L. Francone
ABCA1-Deficient Mice: Insights Into the Role of Monocyte Lipid Efflux in HDL Formation and Inflammation
Arterioscler. Thromb. Vasc. Biol., June 1, 2003; 23(6): 972 - 980.
[Abstract] [Full Text] [PDF]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
C. Joyce, L. Freeman, H. B. Brewer Jr, and S. Santamarina-Fojo
Study of ABCA1 Function in Transgenic Mice
Arterioscler. Thromb. Vasc. Biol., June 1, 2003; 23(6): 965 - 971.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. S. Kiss, D. C. McManus, V. Franklin, W. L. Tan, A. McKenzie, G. Chimini, and Y. L. Marcel
The Lipidation by Hepatocytes of Human Apolipoprotein A-I Occurs by Both ABCA1-dependent and -independent Pathways
J. Biol. Chem., March 14, 2003; 278(12): 10119 - 10127.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
S. Altilia, L. Pisciotta, R. Garuti, P. Tarugi, A. Cantafora, L. Calabresi, J. Tagliabue, S. Maccari, F. Bernini, I. Zanotti, et al.
Abnormal splicing of ABCA1 pre-mRNA in Tangier disease due to a IVS2 +5G>C mutation in ABCA1 gene
J. Lipid Res., February 1, 2003; 44(2): 254 - 264.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
C. L. Wellington, Y.-Z. Yang, S. Zhou, S. M. Clee, B. Tan, K. Hirano, K. Zwarts, A. Kwok, A. Gelfer, M. Marcil, et al.
Truncation mutations in ABCA1 suppress normal upregulation of full-length ABCA1 by 9-cis-retinoic acid and 22-R-hydroxycholesterol
J. Lipid Res., November 1, 2002; 43(11): 1939 - 1949.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. Langmann, M. Porsch-Ozcurumez, S. Heimerl, M. Probst, C. Moehle, M. Taher, H. Borsukova, D. Kielar, W. E. Kaminski, E. Dittrich-Wengenroth, et al.
Identification of Sterol-independent Regulatory Elements in the Human ATP-binding Cassette Transporter A1 Promoter. ROLE OF Sp1/3, E-BOX BINDING FACTORS, AND AN ONCOSTATIN M-RESPONSIVE ELEMENT
J. Biol. Chem., April 19, 2002; 277(17): 14443 - 14450.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y. Wang and J. F. Oram
Unsaturated Fatty Acids Inhibit Cholesterol Efflux from Macrophages by Increasing Degradation of ATP-binding Cassette Transporter A1
J. Biol. Chem., February 8, 2002; 277(7): 5692 - 5697.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
A. D. Attie, J. P. Kastelein, and M. R. Hayden
Pivotal role of ABCA1 in reverse cholesterol transport influencing HDL levels and susceptibility to atherosclerosis
J. Lipid Res., November 1, 2001; 42(11): 1717 - 1726.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
S. Santamarina-Fojo, A. T. Remaley, E. B. Neufeld, and H. B. Brewer Jr.
Regulation and intracellular trafficking of the ABCA1 transporter
J. Lipid Res., September 1, 2001; 42(9): 1339 - 1345.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
J. F. Oram and R. M. Lawn
ABCA1: the gatekeeper for eliminating excess tissue cholesterol
J. Lipid Res., August 1, 2001; 42(8): 1173 - 1179.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. R. Singaraja, V. Bocher, E. R. James, S. M. Clee, L.-H. Zhang, B. R. Leavitt, B. Tan, A. Brooks-Wilson, A. Kwok, N. Bissada, et al.
Human ABCA1 BAC Transgenic Mice Show Increased High Density Lipoprotein Cholesterol and ApoAI-dependent Efflux Stimulated by an Internal Promoter Containing Liver X Receptor Response Elements in Intron 1
J. Biol. Chem., August 31, 2001; 276(36): 33969 - 33979.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
C. W. Joyce, M. J. A. Amar, G. Lambert, B. L. Vaisman, B. Paigen, J. Najib-Fruchart, R. F. Hoyt Jr., E. D. Neufeld, A. T. Remaley, D. S. Fredrickson, et al.
The ATP binding cassette transporter A1 (ABCA1) modulates the development of aortic atherosclerosis in C57BL/6 and apoE-knockout mice
PNAS, January 8, 2002; 99(1): 407 - 412.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2001 by the American Society for Biochemistry and Molecular Biology.