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J. Biol. Chem., Vol. 276, Issue 21, 18060-18065, May 25, 2001
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From the Department of Preventive Dentistry, Kyushu University
Faculty of Dental Science, Fukuoka 812-8582, Japan
Human whole saliva induces aggregation of
Streptococcus mutans cells via an interaction between a
surface protein antigen (PAc) of the organism and salivary agglutinin.
Bovine milk inhibits the saliva-induced aggregation of S. mutans. In this study, the milk component that possesses
inhibitory activity against this aggregation was isolated and found to
be lactoferrin. Surface plasmon resonance analysis indicated that
bovine lactoferrin binds more strongly to salivary agglutinin,
especially to high molecular mass glycoprotein, which is a component of
the agglutinin, than to recombinant PAc. The binding of bovine
lactoferrin to salivary agglutinin was thermostable, and the optimal pH
for binding was 4.0. To identify the saliva-binding region of bovine
lactoferrin, 11 truncated bovine lactoferrin fragments were
constructed. A fragment corresponding to the C-terminal half of the
lactoferrin molecule had a strong inhibitory effect on the
saliva-induced aggregation of S. mutans, whereas a fragment
corresponding to the N-terminal half had a weak inhibitory effect.
Seven shorter fragments corresponding to lactoferrin residues 473-538
also showed a high ability to inhibit the aggregation of S. mutans. These results suggest that residues 473-538 of bovine
lactoferrin are important in the inhibition of saliva-induced
aggregation of S. mutans.
Inhibitory Effect of Bovine Milk Lactoferrin on the Interaction
between a Streptococcal Surface Protein Antigen and Human Salivary
Agglutinin*
,
*
This work was supported in part by Grants-in-aid for
Developmental Scientific Research (A)12357013 (to T. K.) and
(C)11672051 (to T. O.) from the Ministry of Education, Science,
Sports and Culture of Japan and by the Kyushu University
Interdisciplinary Programs in Education and Projects in Research
Development (to T. K.).The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
To whom correspondence should be addressed. Tel.: 81-92-642-6353;
Fax: 81-92-642-6354; E-mail: oho@dent.kyushu-u.ac.jp.
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