HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 276, Issue 21, 18139-18145, May 25, 2001

This Article Full Text Full Text (PDF) All Versions of this Article: 276/21/18139    most recent M010621200v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Calderini, O. Articles by Wilson, C. Search for Related Content PubMed PubMed Citation Articles by Calderini, O. Articles by Wilson, C. Social Bookmarking                      What's this?

A Novel Tobacco Mitogen-activated Protein (MAP) Kinase Kinase, NtMEK1, Activates the Cell Cycle-regulated p43^Ntf6 MAP Kinase^*

Ornella Calderini^§¶, Nathalie Glab, Catherine Bergounioux, Erwin Heberle-Bors^**, and Cathal Wilson

From the  Institute of Microbiology and Genetics, University of Vienna, Dr. Bohrgasse 9, A-1030 Vienna, Austria, the ^§ Istituto di Ricerche sul Miglioramento Genetico Piante Foraggere, Consiglio Nazionale delle Ricerche, via Madonna Alta 130, I-06128 Perugia, Italy, and the  Institut de Biotechnologie des Plantes, CNRS ERS 569, Université de Paris-Sud, Bât. 630, Plateau du Moulon, F-91405 Orsay Cedex, France

Two-hybrid screening of a tobacco BY-2 cell suspension cDNA library using the p43^Ntf6 mitogen-activated protein (MAP) kinase as bait resulted in the isolation of a cDNA encoding a protein with features characteristic of a MAP kinase kinase (MEK), which has been called NtMEK1. Two-hybrid interaction analysis and pull-down experiments showed a physical interaction between NtMEK1 and the tobacco MAP kinases p43^Ntf6 and p45^Ntf4, but not p43^Ntf3. In kinase assays NtMEK1 preferentially phosphorylated p43^Ntf6. Functional studies in yeast showed that p43^Ntf6 could complement the yeast MAP kinase mutant mpk1 when co-expressed with NtMEK1, and that this complementation depended on the kinase activity of p43^Ntf6. Expression analysis showed that the NtMEK1 and ntf6 genes are co-expressed both in plant tissues and following the induction of cell division in leaf pieces. These data suggest that NtMEK1 is an MEK for the p43^Ntf6 MAP kinase.

The nucleotide sequence(s) reported in this paper has been submitted to the GenBank^TM/EMBL Data Bank with accession number(s) AJ302651 (NtMEK1).

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				J. Xu, Y. Li, Y. Wang, H. Liu, L. Lei, H. Yang, G. Liu, and D. Ren Activation of MAPK Kinase 9 Induces Ethylene and Camalexin Biosynthesis and Enhances Sensitivity to Salt Stress in Arabidopsis J. Biol. Chem., October 3, 2008; 283(40): 26996 - 27006. [Abstract] [Full Text] [PDF]

				T. Soyano, R. Nishihama, K. Morikiyo, M. Ishikawa, and Y. Machida NQK1/NtMEK1 is a MAPKK that acts in the NPK1 MAPKKK-mediated MAPK cascade and is required for plant cytokinesis Genes & Dev., April 15, 2003; 17(8): 1055 - 1067. [Abstract] [Full Text] [PDF]

				J.-Q. Wen, K. Oono, and R. Imai Two Novel Mitogen-Activated Protein Signaling Components, OsMEK1 and OsMAP1, Are Involved in a Moderate Low-Temperature Signaling Pathway in Rice Plant Physiology, August 1, 2002; 129(4): 1880 - 1891. [Abstract] [Full Text] [PDF]

				F. Cardinale, I. Meskiene, F. Ouaked, and H. Hirt Convergence and Divergence of Stress-Induced Mitogen-Activated Protein Kinase Signaling Pathways at the Level of Two Distinct Mitogen-Activated Protein Kinase Kinases PLANT CELL, March 1, 2002; 14(3): 703 - 711. [Abstract] [Full Text] [PDF]