| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 276, Issue 21, 18200-18208, May 25, 2001
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
From the § Division of Cell Biology, Hospital for Sick
Children, Toronto, Ontario M5G 1X8, Canada, the ¶ Department of
Surgery, Toronto General Hospital, Toronto, Ontario M5G 2C4, Canada,
the Recent evidence suggests that
extension of pseudopods during phagocytosis requires localized
insertion of endomembrane vesicles. The nature of these vesicles and
the processes mediating their release and insertion are unknown. COPI
plays an essential role in the budding and traffic of membrane vesicles
in intracellular compartments. We therefore assessed whether COPI is
also involved in phagosome formation. We used ldlF cells, a mutant line
derived from Chinese hamster ovary cells that express a
temperature-sensitive form of
Indirect Role for COPI in the Completion of Fc
Receptor-mediated Phagocytosis*
§¶**,§,
,
Department of Physiology, The Ohio State University,
Columbus, Ohio 43210, and the ¶¶ Department of Medicine,
University of Pennsylvania,
Philadelphia, Pennsylvania 19104-4283
COP. To confer phagocytic ability to
ldlF cells, they were stably transfected with Fc receptors type IIA
(Fc
RIIA). In the presence of functional COPI, FcRIIA-transfected
ldlF cells effectively internalized opsonized particles. In contrast,
phagocytosis was virtually eliminated after incubation at the
restrictive temperature. Similar results were obtained impairing COPI
function in macrophages using brefeldin A. Notably, loss of COPI
function preceded complete inhibition of phagocytosis, suggesting that
COPI is indirectly required for phagocytosis. Despite their inability
to internalize particles, COPI-deficient cells nevertheless expressed
normal levels of FcRIIA, and signal transduction appeared unimpeded. The opsonized particles adhered normally to COPI-deficient cells and
were often found on actin-rich pedestals, but they were not internalized due to the inability of the cells to extend pseudopods. The failure to extend pseudopods was attributed to the inability of COPI-deficient cells to mobilize endomembrane vesicles, including a
VAMP3-containing compartment, in response to the phagocytic stimulus.
*
This work was supported by the Medical Research Council
(MRC), the Arthritis Society, and National Sanatorium Association.The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
These authors contributed equally to this work.
**
Recipient of a postdoctoral fellowship from the MRC. Present
address: Dept. of Pediatric Surgery, Childrens Hospital of Pittsburgh, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213.
Recipient of a graduate studentship from the Natural Sciences
and Engineering Research Council of Canada.
An MRC Distinguished Scientist, an International
Scholar of the Howard Hughes Medical Institute, and the current holder
of the Pitblado Chair in Cell Biology. To whom correspondence should be
addressed: Division of Cell Biology, Hospital for Sick Children, 555 University Ave., Toronto, Ontario M5G 1X8, Canada. Tel.: 416-813-5727; Fax: 416-813-5028; E-mail: sga@sickkids.on.ca.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  T. Becker, A. Volchuk, and J. E. Rothman Differential use of endoplasmic reticulum membrane for phagocytosis in J774 macrophages PNAS, March 15, 2005; 102(11): 4022 - 4026. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Lefkir, M. Malbouyres, D. Gotthardt, A. Ozinsky, S. Cornillon, F. Bruckert, A. A. Aderem, T. Soldati, P. Cosson, and F. Letourneur Involvement of the AP-1 Adaptor Complex in Early Steps of Phagocytosis and Macropinocytosis Mol. Biol. Cell, February 1, 2004; 15(2): 861 - 869. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. C. Larsen, T. Ueyama, P. M. Brannock, Y. Shirai, N. Saito, C. Larsson, D. Loegering, P. B. Weber, and M. R. Lennartz A role for PKC-{varepsilon} in Fc{gamma}R-mediated phagocytosis by RAW 264.7 cells J. Cell Biol., December 23, 2002; 159(6): 939 - 944. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Garcia-Garcia and C. Rosales Signal transduction during Fc receptor-mediated phagocytosis J. Leukoc. Biol., December 1, 2002; 72(6): 1092 - 1108. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Gotthardt, H. J. Warnatz, O. Henschel, F. Bruckert, M. Schleicher, and T. Soldati High-Resolution Dissection of Phagosome Maturation Reveals Distinct Membrane Trafficking Phases Mol. Biol. Cell, October 1, 2002; 13(10): 3508 - 3520. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. A. Fratti, J. Chua, and V. Deretic Cellubrevin Alterations and Mycobacterium tuberculosis Phagosome Maturation Arrest J. Biol. Chem., May 3, 2002; 277(19): 17320 - 17326. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
