| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 276, Issue 21, 18265-18271, May 25, 2001
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
From Genesis Research and Development Corporation Limited,
P. O. Box 50, Auckland 1015, New Zealand and
§ Genometrix Incorporated, The Woodlands, Texas 77381
High throughput sequencing of a mouse
keratinocyte library was used to identify an expressed sequence tag
with homology to the epidermal growth factor (EGF) family of growth
factors. We have named the protein encoded by this expressed sequence
tag Epigen, for epithelial mitogen. Epigen encodes a protein of 152 amino acids that contains features characteristic of the EGF
superfamily. Two hydrophobic regions, corresponding to a putative
signal sequence and transmembrane domain, flank a core of amino acids
encompassing six cysteine residues and two putative
N-linked glycosylation sites. Epigen shows 24-37%
identity to members of the EGF superfamily including EGF,
transforming growth factor The nucleotide sequence(s) reported in this paper has been submitted to the GenBankTM/EMBL Data Bank with accession number(s) AJ291391.
Cloning and Biological Activity of Epigen, a Novel Member of the
Epidermal Growth Factor Superfamily*
,
, and Epiregulin. Northern blotting of several adult mouse tissues indicated that Epigen was
present in testis, heart, and liver. Recombinant Epigen was synthesized
in Escherichia coli and refolded, and its biological activity was compared with that of EGF and transforming growth factor
in several assays. In epithelial cells, Epigen stimulated the
phosphorylation of c-erbB-1 and mitogen-activated protein kinases and also activated a reporter gene containing enhancer sequences present in the c-fos promoter. Epigen also
stimulated the proliferation of HaCaT cells, and this
proliferation was blocked by an antibody to the extracellular domain of
the receptor tyrosine kinase c-erbB-1. Thus, Epigen is
the newest member of the EGF superfamily and, with its ability to
promote the growth of epithelial cells, may constitute a novel
molecular target for wound-healing therapy.
*
The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
To whom correspondence should be addressed. Tel.: 64-9-373-5600;
Fax: 64-9-373-2189; E-mail: l.strachan@genesis.co.nz.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  M. R. Schneider, S. Werner, R. Paus, and E. Wolf Beyond Wavy Hairs: The Epidermal Growth Factor Receptor and Its Ligands in Skin Biology and Pathology Am. J. Pathol., July 1, 2008; 173(1): 14 - 24. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Widakowich, G. de Castro Jr., E. de Azambuja, P. Dinh, and A. Awada Review: Side Effects of Approved Molecular Targeted Therapies in Solid Cancers Oncologist, December 1, 2007; 12(12): 1443 - 1455. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. L. Streicher, N. E. Willmarth, J. Garcia, J. L. Boerner, T. G. Dewey, and S. P. Ethier Activation of a Nuclear Factor {kappa}B/Interleukin-1 Positive Feedback Loop by Amphiregulin in Human Breast Cancer Cells Mol. Cancer Res., August 1, 2007; 5(8): 847 - 861. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. R. Pochampalli, B. G. Bitler, and J. A. Schroeder Transforming Growth Factor {alpha} Dependent Cancer Progression Is Modulated by Muc1 Cancer Res., July 15, 2007; 67(14): 6591 - 6598. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Chen, J.-K. Chen, J. R. Falck, S. Anjaiah, J. H. Capdevila, and R. C. Harris Mitogenic Activity and Signaling Mechanism of 2-(14,15- Epoxyeicosatrienoyl)Glycerol, a Novel Cytochrome P450 Arachidonate Metabolite Mol. Cell. Biol., April 15, 2007; 27(8): 3023 - 3034. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. C Woods and A L Johnson Protein kinase C activity mediates LH-induced ErbB/Erk signaling in differentiated hen granulosa cells Reproduction, April 1, 2007; 133(4): 733 - 741. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. E. Willmarth and S. P. Ethier Autocrine and Juxtacrine Effects of Amphiregulin on the Proliferative, Invasive, and Migratory Properties of Normal and Neoplastic Human Mammary Epithelial Cells J. Biol. Chem., December 8, 2006; 281(49): 37728 - 37737. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. M. Casalino-Matsuda, M. E. Monzon, and R. M. Forteza Epidermal Growth Factor Receptor Activation by Epidermal Growth Factor Mediates Oxidant-Induced Goblet Cell Metaplasia in Human Airway Epithelium Am. J. Respir. Cell Mol. Biol., May 1, 2006; 34(5): 581 - 591. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Boxall, S. T. Holgate, and D. E. Davies The contribution of transforming growth factor-{beta} and epidermal growth factor signalling to airway remodelling in chronic asthma Eur. Respir. J., January 1, 2006; 27(1): 208 - 229. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Tanaka, S. Miyamoto, S. O. Suzuki, E. Oki, H. Yagi, K. Sonoda, A. Yamazaki, H. Mizushima, Y. Maehara, E. Mekada, et al. Clinical Significance of Heparin-Binding Epidermal Growth Factor-Like Growth Factor and A Disintegrin and Metalloprotease 17 Expression in Human Ovarian Cancer Clin. Cancer Res., July 1, 2005; 11(13): 4783 - 4792. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Dong, L. K. Opresko, W. Chrisler, G. Orr, R. D. Quesenberry, D. A. Lauffenburger, and H. S. Wiley The Membrane-anchoring Domain of Epidermal Growth Factor Receptor Ligands Dictates Their Ability to Operate in Juxtacrine Mode Mol. Biol. Cell, June 1, 2005; 16(6): 2984 - 2998. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. S. Kochupurakkal, D. Harari, A. Di-Segni, G. Maik-Rachline, L. Lyass, G. Gur, G. Kerber, A. Citri, S. Lavi, R. Eilam, et al. Epigen, the Last Ligand of ErbB Receptors, Reveals Intricate Relationships between Affinity and Mitogenicity J. Biol. Chem., March 4, 2005; 280(9): 8503 - 8512. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Whitsett, C. J. Bachurski, K. C. Barnes, P. A. Bunn Jr., L. M. Case, D. N. Cook, D. Crooks, M. W. Duncan, L. Dwyer-Nield, R. C. Elston, et al. Functional Genomics of Lung Disease Am. J. Respir. Cell Mol. Biol., August 1, 2004; 31(2/S1): S1 - S81. [Full Text] [PDF]
|
|
|
|
 |
|
 K. E. Strunk, V. Amann, and D. W. Threadgill Phenotypic Variation Resulting From a Deficiency of Epidermal Growth Factor Receptor in Mice Is Caused by Extensive Genetic Heterogeneity That Can Be Genetically and Molecularly Partitioned Genetics, August 1, 2004; 167(4): 1821 - 1832. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. M. Casalino-Matsuda, M. E. Monzon, G. E. Conner, M. Salathe, and R. M. Forteza Role of Hyaluronan and Reactive Oxygen Species in Tissue Kallikrein-mediated Epidermal Growth Factor Receptor Activation in Human Airways J. Biol. Chem., May 14, 2004; 279(20): 21606 - 21616. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 U. Sahin, G. Weskamp, K. Kelly, H.-M. Zhou, S. Higashiyama, J. Peschon, D. Hartmann, P. Saftig, and C. P. Blobel Distinct roles for ADAM10 and ADAM17 in ectodomain shedding of six EGFR ligands J. Cell Biol., March 1, 2004; 164(5): 769 - 779. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. IIVANAINEN, L. NELIMARKKA, V. ELENIUS, S.-M. HEIKKINEN, T. T. JUNTTILA, L. SIHOMBING, M. SUNDVALL, J. A. MAATTA, V. J. O. LAINE, S. YLA-HERTTUALA, et al. Angiopoietin-regulated recruitment of vascular smooth muscle cells by endothelial-derived heparin binding EGF-like growth factor FASEB J, September 1, 2003; 17(12): 1609 - 1621. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. K. L. Mak and S. Y. Chan Epidermal Growth Factor as a Biologic Switch in Hair Growth Cycle J. Biol. Chem., July 3, 2003; 278(28): 26120 - 26126. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. WERNER and R. GROSE Regulation of Wound Healing by Growth Factors and Cytokines Physiol Rev, July 1, 2003; 83(3): 835 - 870. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-H. Cha, J. S. Brooke, M. Y. Chang, and L. Eidels Receptor-Based Antidote for Diphtheria Infect. Immun., May 1, 2002; 70(5): 2344 - 2350. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. W. Sunnarborg, C. L. Hinkle, M. Stevenson, W. E. Russell, C. S. Raska, J. J. Peschon, B. J. Castner, M. J. Gerhart, R. J. Paxton, R. A. Black, et al. Tumor Necrosis Factor-alpha Converting Enzyme (TACE) Regulates Epidermal Growth Factor Receptor Ligand Availability J. Biol. Chem., April 5, 2002; 277(15): 12838 - 12845. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
