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Originally published In Press as doi:10.1074/jbc.C100095200 on April 10, 2001

J. Biol. Chem., Vol. 276, Issue 22, 18653-18656, June 1, 2001
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ACCELERATED PUBLICATION
Characterization of the Methylation-sensitive Promoter of the Imprinted ZAC Gene Supports Its Role in Transient Neonatal Diabetes Mellitus*

Annie VarraultDagger , Benoit Bilanges§, Deborah J. G. Mackay, Eugenia Basyuk||, Barbara Ahr, Céline Fernandez, David O. Robinson, Joël Bockaert, and Laurent Journot

From UPR 9023 CNRS-Centre CNRS-INSERM de Pharmacologie-Endocrinologie-141, rue de la Cardonille, 34094 Montpellier Cedex 05, France and  Wessex Regional Genetics Laboratory, Salisbury District Hospital, Salisbury, Wiltshire SP2 8BJ, United Kingdom

ZAC is a recently isolated zinc finger protein that induces apoptosis and cell cycle arrest. The corresponding gene is imprinted maternally through an unknown mechanism and maps to 6q24-q25, within the minimal interval harboring the gene responsible for transient neonatal diabetes mellitus (TNDM) and a tumor suppressor gene involved in breast cancer. Because of its functional properties, imprinting status, and expression pattern in mammary cell lines and tumors, ZAC is the best candidate so far for both disease conditions. In the present work, we delineated ZAC genomic organization and mapped its transcriptional start site. It is noteworthy that the ZAC promoter localized to the CpG island harboring the methylation imprint associated with TNDM and methylation of this promoter silenced its activity. These data indicate that the methylation mark may have a direct effect on the silencing of the ZAC imprinted allele. Our findings further strengthen the hypothesis that ZAC is the gene responsible for TNDM and suggest a novel mechanism for ZAC inactivation in breast tumors.

* This work was supported by grants from the Centre Nationale de la Recherche Scientifique, La Ligue Nationale contre le Cancer, L'Association pour la Recherche contre le Cancer (ARC), the European Commission (QLG3-CT-1999-00602), and Diabetes UK (RD98/0001627).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

§ Recipient of predoctoral fellowships from the Ministère de l'Education Nationale, de la Recherche et de la Technologie and from the ARC.

|| Recipient of postdoctoral fellowships from the Ministère des Affaires Etrangères and the Fondation pour la Recherche Médicale.

Dagger To whom correspondence should be addressed. Tel.: 33-467 142 963; Fax: 33-467 542 432; E-mail: varrault@ccipe.montp.inserm.fr.

Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.


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