JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M100705200 on March 14, 2001

J. Biol. Chem., Vol. 276, Issue 22, 18849-18854, June 1, 2001
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
276/22/18849    most recent
M100705200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Caselli, A.
Right arrow Articles by Ramponi, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Caselli, A.
Right arrow Articles by Ramponi, G.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Tyrosine-phosphorylated Caveolin Is a Physiological Substrate of the Low Mr Protein-Tyrosine Phosphatase*

Anna Caselli, Maria Letizia Taddei, Giampaolo Manao, Guido CamiciDagger , and Giampietro Ramponi

From the Dipartimento di Scienze Biochimiche, Università di Firenze, 50134 Firenze, Italy

Low Mr phosphotyrosine-protein phosphatase is involved in the regulation of several tyrosine kinase growth factor receptors. The best characterized action of this enzyme is on the signaling pathways activated by platelet-derived growth factor, where it plays multiple roles. In this study we identify tyrosine-phosphorylated caveolin as a new potential substrate for low Mr phosphotyrosine-protein phosphatase. Caveolin is tyrosine-phosphorylated in vivo by Src kinases, recruits into caveolae, and hence regulates the activities of several proteins involved in cellular signaling cascades. Our results demonstrate that caveolin and low Mr phosphotyrosine-protein phosphatase coimmunoprecipitate from cell lysates, and that a fraction of the enzyme localizes in caveolae. Furthermore, in a cell line sensitive to insulin, the overexpression of the C12S dominant negative mutant of low Mr phosphotyrosine-protein phosphatase (a form lacking activity but able to bind substrates) causes the enhancement of tyrosine-phosphorylated caveolin. Insulin stimulation of these cells induces a strong increase of caveolin phosphorylation. The localization of low Mr phosphotyrosine-protein phosphatase in caveolae, the in vivo interaction between this enzyme and caveolin, and the capacity of this enzyme to rapidly dephosphorylate phosphocaveolin, all indicate that tyrosine-phosphorylated caveolin is a relevant substrate for this phosphatase.

* This work was supported by Consiglio Nazionale delle Ricerche Strategic Project "Controlli Post-Trascrizionali dell'Espressione Genica" and Target Project on Biotechnology, Ministero dell' Università e della Ricerca Scientifica e Tecnologica (MURST 1997) "Meccanismi Biochimici di Controllo delle Funzioni Cellulari," MURST-CNR Biotechnology Program L.95/95, and the Italian Association for Cancer Research.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Dagger To whom correspondence should be addressed: Dipartimento di Scienze Biochimiche, Università di Firenze, Viale Morgagni 50, 50134 Firenze, Italy. Tel.: 39-055-413765; Fax: 39-055-4222725; E-mail: camici@scibio.unifi.it.

Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
B. P. Head, H. H. Patel, D. M. Roth, F. Murray, J. S. Swaney, I. R. Niesman, M. G. Farquhar, and P. A. Insel
Microtubules and Actin Microfilaments Regulate Lipid Raft/Caveolae Localization of Adenylyl Cyclase Signaling Components
J. Biol. Chem., September 8, 2006; 281(36): 26391 - 26399.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
S. Ikeda, M. Ushio-Fukai, L. Zuo, T. Tojo, S. Dikalov, N. A. Patrushev, and R. W. Alexander
Novel Role of ARF6 in Vascular Endothelial Growth Factor-Induced Signaling and Angiogenesis
Circ. Res., March 4, 2005; 96(4): 467 - 475.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
H. Wang, M. Haas, M. Liang, T. Cai, J. Tian, S. Li, and Z. Xie
Ouabain Assembles Signaling Cascades through the Caveolar Na+/K+-ATPase
J. Biol. Chem., April 23, 2004; 279(17): 17250 - 17259.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
L. Labrecque, I. Royal, D. S. Surprenant, C. Patterson, D. Gingras, and R. Beliveau
Regulation of Vascular Endothelial Growth Factor Receptor-2 Activity by Caveolin-1 and Plasma Membrane Cholesterol
Mol. Biol. Cell, January 1, 2003; 14(1): 334 - 347.
[Abstract] [Full Text]

Home page
 J. Lipid Res.Home page
J. C. Cruz, M. Thomas, E. Wong, N. Ohgami, S. Sugii, T. Curphey, C. C. Y. Chang, and T.-Y. Chang
Synthesis and biochemical properties of a new photoactivatable cholesterol analog 7,7-azocholestanol and its linoleate ester in Chinese hamster ovary cell lines
J. Lipid Res., August 1, 2002; 43(8): 1341 - 1347.
[Abstract] [Full Text] [PDF]

Home page
 J. Gen. Virol.Home page
G. Brown, H. W. McL. Rixon, and R. J. Sugrue
Respiratory syncytial virus assembly occurs in GM1-rich regions of the host-cell membrane and alters the cellular distribution of tyrosine phosphorylated caveolin-1
J. Gen. Virol., August 1, 2002; 83(8): 1841 - 1850.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2001 by the American Society for Biochemistry and Molecular Biology.