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J. Biol. Chem., Vol. 276, Issue 23, 20022-20028, June 8, 2001
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From the German Cancer Research Center, Division of Immunochemistry
(G0200), Im Neuenheimer Feld 280, 69120 Heidelberg, Germany
Here we show that in human T-cell leukemia cells
Vav1 and protein kinase C
Protein Kinase C
Cooperates with Vav1 to Induce JNK
Activity in T-cells*
(PKC) synergize for the activation of
c-Jun N-terminal kinase (JNK) but not p38 MAP kinase. Vav1 and PKC also cooperated to induce transcription of reporter genes controlled either by AP-1 binding sites or the CD28RE/AP composite element contained in the IL-2 promoter by stimulating the binding of
transcription factors to these two elements. Dominant negative
versions of Vav1 and PKC inhibited CD3/CD28-induced activation of
JNK, revealing their relative importance for this activation pathway.
Gel filtration experiments revealed the existence of constitutively
associated Vav1/PKC heterodimers in extracts from unstimulated
T-cells, whereas T-cell costimulation induced the recruitment of Vav1
into high molecular weight complexes. Several experimental approaches showed that Vav1 is located upstream from PKC in the control of the
pathway leading to synergistic JNK activation. Vav1-derived signals
lead to the activation of JNK by at least two different pathways. The major contribution of Vav1 for the activation of JNK relies on the PKC-mediated Ca2+-independent
synergistic activation pathway, whereas JNK is also activated by a
separate Ca2+-dependent signaling route.
*
This work was supported by grants from the Land
Baden-Württemberg, European Union (QLK3-2000-00463), Fonds
der Chemischen Industrie, Deutsche Forschungsgemeinschaft (Schm
1417/3-1), and Deutsche Krebshilfe.The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
To whom correspondence should be addressed. Tel.: 49-6221-423725;
Fax: 49-6221-423746; E-mail: L.Schmitz@DKFZ.de.
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