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Originally published In Press as doi:10.1074/jbc.M101140200 on March 23, 2001

J. Biol. Chem., Vol. 276, Issue 23, 20474-20481, June 8, 2001
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Dual Targeting of Spinach Protoporphyrinogen Oxidase II to Mitochondria and Chloroplasts by Alternative Use of Two In-frame Initiation Codons*

Naohide WatanabeDagger §, Fang-Sik CheDagger ||, Megumi IwanoDagger , Seiji TakayamaDagger , Shigeo Yoshida**, and Akira IsogaiDagger

From the Dagger  Graduate School of Biological Sciences, Nara Institute of Science and Technology, Takayama 8916-5, Ikoma, Nara 630-0101 and the ** Plant Function Laboratory, The Institute of Physical and Chemical Research, Hirosawa 2-1, Wako, Saitama 351-0198, Japan

Protoporphyrinogen oxidase (Protox) is the final enzyme in the common pathway of chlorophyll and heme biosynthesis. Two Protox isoenzymes have been described in tobacco, a plastidic and a mitochondrial form. We isolated and sequenced spinach Protox cDNA, which encodes a homolog of tobacco mitochondrial Protox (Protox II). Alignment of the deduced amino acid sequence between Protox II and other tobacco mitochondrial Protox homologs revealed a 26-amino acid N-terminal extension unique to the spinach enzyme. Immunoblot analysis of spinach leaf extract detected two proteins with apparent molecular masses of 57 and 55 kDa in chloroplasts and mitochondria, respectively. In vitro translation experiments indicated that two translation products (59 and 55 kDa) are produced from Protox II mRNA, using two in-frame initiation codons. Transport experiments using green fluorescent protein-fused Protox II suggested that the larger and smaller translation products (Protox IIL and IIS) target exclusively to chloroplasts and mitochondria, respectively.

* This work was supported in part by a Grant-in-aid for Encouragement of Young Scientists 09760304 from the Ministry of Education, Science, Sports and Culture of Japan.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

The nucleotide sequence reported in this paper has been submitted to the DDBJ/GenBankTM/EBI Data Bank with accession number AB046993.

§ Recipient of postdoctoral research fellowships from the Japan Society for the Promotion of Science for Young Scientists.

The first two authors contributed equally to this work.

|| To whom correspondence should be addressed. Tel.: 81-743-72-5452; Fax: 81-743-72-5459; E-mail: fsche@bs.aist-nara.ac.jp.

Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.
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