JBC Avanti Polar Lipids

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M100088200 on February 27, 2001

J. Biol. Chem., Vol. 276, Issue 23, 20727-20734, June 8, 2001
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
276/23/20727    most recent
M100088200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Chong, S. S.
Right arrow Articles by Hernandez, N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Chong, S. S.
Right arrow Articles by Hernandez, N.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Reconstitution of Transcription from the Human U6 Small Nuclear RNA Promoter with Eight Recombinant Polypeptides and a Partially Purified RNA Polymerase III Complex*

Setareh Sepehri ChongDagger , Ping Hu§, and Nouria Hernandez||**

From the Dagger  Department of Microbiology and § Graduate Program of Molecular and Cellular Biology, State University of New York at Stony Brook, Stony Brook, New York 11794, and the  Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724

The human U6 small nuclear (sn) RNA core promoter consists of a proximal sequence element, which recruits the multisubunit factor SNAPc, and a TATA box, which recruits the TATA box-binding protein, TBP. In addition to SNAPc and TBP, transcription from the human U6 promoter requires two well defined factors. The first is hB", a human homologue of the B" subunit of yeast TFIIIB generally required for transcription of RNA polymerase III genes, and the second is hBRFU, one of two human homologues of the yeast TFIIIB subunit BRF specifically required for transcription of U6-type RNA polymerase III promoters. Here, we have partially purified and characterized a RNA polymerase III complex that can direct transcription from the human U6 promoter when combined with recombinant SNAPc, recombinant TBP, recombinant hB", and recombinant hBRFU. These results open the way to reconstitution of U6 transcription from entirely defined components.

* This work was supported in part by National Institutes of Health Grant GM38810.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

|| Supported by the Howard Hughes Medical Institute.

** To whom correspondence should be addressed.

Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
C. Ducrot, O. Lefebvre, E. Landrieux, J. Guirouilh-Barbat, A. Sentenac, and J. Acker
Reconstitution of the Yeast RNA Polymerase III Transcription System with All Recombinant Factors
J. Biol. Chem., April 28, 2006; 281(17): 11685 - 11692.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
P. Hu, S. Wu, and N. Hernandez
A role for {beta}-actin in RNA polymerase III transcription
Genes & Dev., December 15, 2004; 18(24): 3010 - 3015.
[Abstract] [Full Text] [PDF]

Home page
 JNCI J Natl Cancer InstHome page
B. Spankuch, Y. Matthess, R. Knecht, B. Zimmer, M. Kaufmann, and K. Strebhardt
Cancer Inhibition in Nude Mice After Systemic Application of U6 Promoter-Driven Short Hairpin RNAs Against PLK1
J Natl Cancer Inst, June 2, 2004; 96(11): 862 - 872.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
A. M. Domitrovich and G. R. Kunkel
Multiple, dispersed human U6 small nuclear RNA genes with varied transcriptional efficiencies
Nucleic Acids Res., May 1, 2003; 31(9): 2344 - 2352.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
S. Weser, J. Riemann, K. H. Seifart, and W. Meissner
Assembly and isolation of intermediate steps of transcription complexes formed on the human 5S rRNA gene
Nucleic Acids Res., May 1, 2003; 31(9): 2408 - 2416.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
P. Hu, S. Wu, Y. Sun, C.-C. Yuan, R. Kobayashi, M. P. Myers, and N. Hernandez
Characterization of Human RNA Polymerase III Identifies Orthologues for Saccharomyces cerevisiae RNA Polymerase III Subunits
Mol. Cell. Biol., November 15, 2002; 22(22): 8044 - 8055.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
L. Schramm and N. Hernandez
Recruitment of RNA polymerase III to its target promoters
Genes & Dev., October 15, 2002; 16(20): 2593 - 2620.
[Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
P. Cabart and S. Murphy
Assembly of Human Small Nuclear RNA Gene-specific Transcription Factor IIIB Complex de Novo On and Off Promoter
J. Biol. Chem., July 19, 2002; 277(30): 26831 - 26838.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
P. J. Paddison, A. A. Caudy, E. Bernstein, G. J. Hannon, and D. S. Conklin
Short hairpin RNAs (shRNAs) induce sequence-specific silencing in mammalian cells
Genes & Dev., April 15, 2002; 16(8): 948 - 958.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
W. Meissner, R. Thomae, and K. H. Seifart
The Activity of Transcription Factor IIIC1 Is Impaired during Differentiation of F9 Cells
J. Biol. Chem., February 22, 2002; 277(9): 7148 - 7156.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
P. Cabart and S. Murphy
BRFU, a TFIIB-like Factor, Is Directly Recruited to the TATA-box of Polymerase III Small Nuclear RNA Gene Promoters through Its Interaction with TATA-binding Protein
J. Biol. Chem., November 9, 2001; 276(46): 43056 - 43064.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
N. Hernandez
Small Nuclear RNA Genes: a Model System to Study Fundamental Mechanisms of Transcription
J. Biol. Chem., July 13, 2001; 276(29): 26733 - 26736.
[Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2001 by the American Society for Biochemistry and Molecular Biology.