|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 276, Issue 23, 20727-20734, June 8, 2001
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
From the The human U6 small nuclear (sn) RNA core promoter
consists of a proximal sequence element, which recruits the
multisubunit factor SNAPc, and a TATA box, which
recruits the TATA box-binding protein, TBP. In addition to
SNAPc and TBP, transcription from the human U6 promoter
requires two well defined factors. The first is hB", a human homologue
of the B" subunit of yeast TFIIIB generally required for transcription
of RNA polymerase III genes, and the second is hBRFU, one of two human
homologues of the yeast TFIIIB subunit BRF specifically required for
transcription of U6-type RNA polymerase III promoters. Here, we have
partially purified and characterized a RNA polymerase III complex that
can direct transcription from the human U6 promoter when combined with
recombinant SNAPc, recombinant TBP, recombinant hB", and
recombinant hBRFU. These results open the way to reconstitution of U6
transcription from entirely defined components.
Reconstitution of Transcription from the Human U6 Small
Nuclear RNA Promoter with Eight Recombinant Polypeptides and
a Partially Purified RNA Polymerase III Complex*
,
**
Department of Microbiology and
§ Graduate Program of Molecular and Cellular Biology,
State University of New York at Stony Brook, Stony Brook, New York
11794, and the ¶ Howard Hughes Medical Institute, Cold Spring
Harbor Laboratory, Cold Spring Harbor, New York 11724
*
This work was supported in part by National Institutes of
Health Grant GM38810.The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
Supported by the Howard Hughes Medical Institute.
**
To whom correspondence should be addressed.
Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  C. Ducrot, O. Lefebvre, E. Landrieux, J. Guirouilh-Barbat, A. Sentenac, and J. Acker Reconstitution of the Yeast RNA Polymerase III Transcription System with All Recombinant Factors J. Biol. Chem., April 28, 2006; 281(17): 11685 - 11692. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Hu, S. Wu, and N. Hernandez A role for {beta}-actin in RNA polymerase III transcription Genes & Dev., December 15, 2004; 18(24): 3010 - 3015. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Spankuch, Y. Matthess, R. Knecht, B. Zimmer, M. Kaufmann, and K. Strebhardt Cancer Inhibition in Nude Mice After Systemic Application of U6 Promoter-Driven Short Hairpin RNAs Against PLK1 J Natl Cancer Inst, June 2, 2004; 96(11): 862 - 872. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. M. Domitrovich and G. R. Kunkel Multiple, dispersed human U6 small nuclear RNA genes with varied transcriptional efficiencies Nucleic Acids Res., May 1, 2003; 31(9): 2344 - 2352. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Weser, J. Riemann, K. H. Seifart, and W. Meissner Assembly and isolation of intermediate steps of transcription complexes formed on the human 5S rRNA gene Nucleic Acids Res., May 1, 2003; 31(9): 2408 - 2416. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Hu, S. Wu, Y. Sun, C.-C. Yuan, R. Kobayashi, M. P. Myers, and N. Hernandez Characterization of Human RNA Polymerase III Identifies Orthologues for Saccharomyces cerevisiae RNA Polymerase III Subunits Mol. Cell. Biol., November 15, 2002; 22(22): 8044 - 8055. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Schramm and N. Hernandez Recruitment of RNA polymerase III to its target promoters Genes & Dev., October 15, 2002; 16(20): 2593 - 2620. [Full Text] [PDF]
|
|
|
|
|
|
P. Cabart and S. Murphy Assembly of Human Small Nuclear RNA Gene-specific Transcription Factor IIIB Complex de Novo On and Off Promoter J. Biol. Chem., July 19, 2002; 277(30): 26831 - 26838. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. J. Paddison, A. A. Caudy, E. Bernstein, G. J. Hannon, and D. S. Conklin Short hairpin RNAs (shRNAs) induce sequence-specific silencing in mammalian cells Genes & Dev., April 15, 2002; 16(8): 948 - 958. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Meissner, R. Thomae, and K. H. Seifart The Activity of Transcription Factor IIIC1 Is Impaired during Differentiation of F9 Cells J. Biol. Chem., February 22, 2002; 277(9): 7148 - 7156. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Cabart and S. Murphy BRFU, a TFIIB-like Factor, Is Directly Recruited to the TATA-box of Polymerase III Small Nuclear RNA Gene Promoters through Its Interaction with TATA-binding Protein J. Biol. Chem., November 9, 2001; 276(46): 43056 - 43064. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Hernandez Small Nuclear RNA Genes: a Model System to Study Fundamental Mechanisms of Transcription J. Biol. Chem., July 13, 2001; 276(29): 26733 - 26736. [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |