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Originally published In Press as doi:10.1074/jbc.M010643200 on March 30, 2001

J. Biol. Chem., Vol. 276, Issue 24, 21235-21241, June 15, 2001
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Antizyme Regulates the Degradation of Ornithine Decarboxylase in Fission Yeast Schizosaccharomyces pombe
STUDY IN THE spe2 KNOCKOUT STRAINS*

Manas K. ChattopadhyayDagger , Yasuko Murakami, and Senya Matsufuji§

From the Department of Biochemistry II, Jikei University School of Medicine, 3-25-8, Nishi-Shinbashi, Minato-ku, Tokyo 105-8461, Japan

The mechanism of the regulatory degradation of ornithine decarboxylase (ODC) by polyamines was studied in fission yeast, Schizosaccharomyces pombe. To regulate cellular spermidine experimentally, we cloned and disrupted S-adenosylmethionine decarboxylase gene (spe2) in S. pombe. The null mutant of spe2 was devoid of spermidine and spermine, accumulated putrescine, and contained a high level of ODC. Addition of spermidine to the culture medium resulted in rapid decrease in the ODC activity caused by the acceleration of ODC degradation, which was dependent on de novo protein synthesis. A fraction of ODC forming an inactive complex concomitantly increased. The accelerated ODC degradation was prevented either by knockout of antizyme gene or by selective inhibitors of proteasome. Thus, unlike budding yeast, mammalian type antizyme-mediated ODC degradation by proteasome is operating in S. pombe.

* This work was supported in part by grants-in-aid from the Ministry of Education, Science and Culture of Japan (to S. M. and Y. M.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

The nucleotide sequences reported in this paper have been submitted to the DDBJ/GenBankTM/EBI Data Bank with accession numbers AB045110 (cDNA) and AB045111 (genomic DNA).

Dagger Postdoctoral fellow of the Japan Society for the Promotion of Science. Present address: Laboratory of Biochemical Genetics, NIDDK, National Institutes of Health, Bethesda, MD 20892-0830.

§ To whom correspondence should be addressed. Tel.: 81-3-3433-1111; Fax: 81-3-3436-3897; E-mail: senya@jikei.ac.jp.

Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.
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