|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 276, Issue 24, 21434-21442, June 15, 2001
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
From the A recombinant human prostasin serine protease was
expressed in several human cell lines. Subcellular fractionation showed that this serine protease is synthesized as a membrane-bound
protein while a free-form prostasin is secreted into the culture
medium. Prostasin was identified in nuclear and membrane fractions.
Membrane-bound prostasin can be released by
phosphatidylinositol-specific phospholipase C treatment, or labeled by
[3H]ethanolamine, indicating a
glycosylphosphatidylinositol anchorage. A prostasin-binding protein was
identified in mouse and human seminal vesicle fluid. Both the secreted
and the membrane-bound prostasin were able to form a covalently linked
82-kDa complex when incubated with seminal vesicle fluid. The complex
formation between prostasin and the prostasin-binding protein was
inhibited by a prostasin antibody, heparin, and serine protease
inhibitors. Prostasin's serine protease activity was inhibited when
bound to the prostasin-binding protein in mouse seminal vesicle fluid. This study indicates that prostasin is an active serine protease in its
membrane-bound form.
Prostasin Is a Glycosylphosphatidylinositol-anchored Active
Serine Protease*
,,,
Department of Molecular Biology and
Microbiology and the ¶ Department of Biology, University of
Central Florida, Orlando, Florida 32816 and the § Department
of Biochemistry and Molecular Biology, Medical University of South
Carolina, Charleston, South Carolina 29425
*
This work was supported by Department of Defense Prostate
Cancer Research Program Grant DAMD17-98-1-8590.The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
To whom correspondence should be addressed: Dept. of Molecular
Biology and Microbiology, University of Central Florida, 4000 Central
Florida Blvd., Orlando, FL 32816-2360. Tel.: 407-823-6122; Fax:
407-823-3095; E-mail: kxchai@mail.ucf.edu.
Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  K. W. Rickert, P. Kelley, N. J. Byrne, R. E. Diehl, D. L. Hall, A. M. Montalvo, J. C. Reid, J. M. Shipman, B. W. Thomas, S. K. Munshi, et al. Structure of Human Prostasin, a Target for the Regulation of Hypertension J. Biol. Chem., December 12, 2008; 283(50): 34864 - 34872. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. M. Myerburg, E. E. McKenna, C. J. Luke, R. A. Frizzell, T. R. Kleyman, and J. M. Pilewski Prostasin expression is regulated by airway surface liquid volume and is increased in cystic fibrosis Am J Physiol Lung Cell Mol Physiol, May 1, 2008; 294(5): L932 - L941. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. List, B. Currie, T. C. Scharschmidt, R. Szabo, J. Shireman, A. Molinolo, B. F. Cravatt, J. Segre, and T. H. Bugge Autosomal Ichthyosis with Hypotrichosis Syndrome Displays Low Matriptase Proteolytic Activity and Is Phenocopied in ST14 Hypomorphic Mice J. Biol. Chem., December 14, 2007; 282(50): 36714 - 36723. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Zhang, H.-Y. Lin, Q. Yang, H.-X. Wang, K. X. Chai, L.-M. Chen, and C. Zhu Expression of Prostasin Serine Protease and Protease Nexin-1 (PN-1) in Rhesus Monkey Ovary During Menstrual Cycle and Early Pregnancy J. Histochem. Cytochem., December 1, 2007; 55(12): 1237 - 1244. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Mori, G. Ishikawa, S.-S. Luo, T. Mishima, T. Goto, J. M. Robinson, S. Matsubara, T. Takeshita, H. Kataoka, and T. Takizawa The Cytotrophoblast Layer of Human Chorionic Villi Becomes Thinner but Maintains Its Structural Integrity During Gestation Biol Reprod, January 1, 2007; 76(1): 164 - 172. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. M. Verghese, M. F. Gutknecht, and G. H. Caughey Prostasin regulates epithelial monolayer function: cell-specific Gpld1-mediated secretion and functional role for GPI anchor Am J Physiol Cell Physiol, December 1, 2006; 291(6): C1258 - C1270. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H.-Y. Lin, H. Zhang, Q. Yang, H.-X. Wang, H.-M. Wang, K. X. Chai, L.-M. Chen, and C. Zhu Expression of Prostasin and Protease Nexin-1 in Rhesus Monkey (Macaca mulatta) Endometrium and Placenta During Early Pregnancy J. Histochem. Cytochem., October 1, 2006; 54(10): 1139 - 1147. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L.-M. Chen, C. Wang, M. Chen, M. R. Marcello, J. Chao, L. Chao, and K. X. Chai Prostasin attenuates inducible nitric oxide synthase expression in lipopolysaccharide-induced urinary bladder inflammation Am J Physiol Renal Physiol, September 1, 2006; 291(3): F567 - F577. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Fan, T. D. Wu, W. Li, and D. Kirchhofer Identification of Hepatocyte Growth Factor Activator Inhibitor-1B as a Potential Physiological Inhibitor of Prostasin J. Biol. Chem., October 14, 2005; 280(41): 34513 - 34520. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Planes, C. Leyvraz, T. Uchida, M. A. Angelova, G. Vuagniaux, E. Hummler, M. Matthay, C. Clerici, and B. Rossier In vitro and in vivo regulation of transepithelial lung alveolar sodium transport by serine proteases Am J Physiol Lung Cell Mol Physiol, June 1, 2005; 288(6): L1099 - L1109. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Tong, B. Illek, V. J. Bhagwandin, G. M. Verghese, and G. H. Caughey Prostasin, a membrane-anchored serine peptidase, regulates sodium currents in JME/CF15 cells, a cystic fibrosis airway epithelial cell line Am J Physiol Lung Cell Mol Physiol, November 1, 2004; 287(5): L928 - L935. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. C. Rossier The Epithelial Sodium Channel: Activation by Membrane-Bound Serine Proteases Proceedings of the ATS, January 1, 2004; 1(1): 4 - 9. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Wang, J. Chao, and L. Chao Adenovirus-mediated human prostasin gene delivery is linked to increased aldosterone production and hypertension in rats Am J Physiol Regulatory Integrative Comp Physiol, April 1, 2003; 284(4): R1031 - R1036. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Iwashita, K. Kitamura, T. Narikiyo, M. Adachi, N. Shiraishi, T. Miyoshi, J. Nagano, D. G. Tuyen, H. Nonoguchi, and K. Tomita Inhibition of Prostasin Secretion by Serine Protease Inhibitors in the Kidney J. Am. Soc. Nephrol., January 1, 2003; 14(1): 11 - 16. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Vallet, C. Pfister, J. Loffing, and B. C. Rossier Cell-Surface Expression of the Channel Activating Protease xCAP-1 Is Required for Activation of ENaC in the Xenopus Oocyte J. Am. Soc. Nephrol., March 1, 2002; 13(3): 588 - 594. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. H. Donaldson, A. Hirsh, D. C. Li, G. Holloway, J. Chao, R. C. Boucher, and S. E. Gabriel Regulation of the Epithelial Sodium Channel by Serine Proteases in Human Airways J. Biol. Chem., March 1, 2002; 277(10): 8338 - 8345. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |