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J. Biol. Chem., Vol. 276, Issue 24, 21670-21677, June 15, 2001

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Human BIN3 Complements the F-actin Localization Defects Caused by Loss of Hob3p, the Fission Yeast Homolog of Rvs161p^*

Eric L. Routhier, Timothy C. Burn^§, Ilgar Abbaszade^§, Matthew Summers^¶, Charles F. Albright, and George C. Prendergast^¶

From the  Cancer Research Group, DuPont Pharmaceuticals Company, Glenolden Laboratory, Glenolden, Pennsylvania 19036, the ^§ Applied Biotechnology Group, DuPont Pharmaceuticals Company, Wilmington, Delaware 19803, and ^¶ The Wistar Institute, Philadelphia, Pennsylvania 19104

The BAR adaptor proteins encoded by the RVS167 and RVS161 genes from Saccharomyces cerevisiae form a complex that regulates actin, endocytosis, and viability following starvation or osmotic stress. In this study, we identified a human homolog of RVS161, termed BIN3 (bridging integrator-3), and a Schizosaccharomyces pombe homolog of RVS161, termed hob3+ (homolog of Bin3). In human tissues, the BIN3 gene was expressed ubiquitously except for brain. S. pombe cells lacking Hob3p were often multinucleate and characterized by increased amounts of calcofluor-stained material and mislocalized F-actin. For example, while wild-type cells localized F-actin to cell ends during interphase, hob3 mutants had F-actin patches distributed randomly around the cell. In addition, medial F-actin rings were rarely found in hob3 mutants. Notably, in contrast to S. cerevisiae rvs161 mutants, hob3 mutants showed no measurable defects in endocytosis or response to osmotic stress, yet hob3+ complemented the osmosensitivity of a rvs161 mutant. BIN3 failed to rescue the osmosensitivity of rvs161, but the actin localization defects of hob3 mutants were completely rescued by BIN3 and partially rescued by RVS161. These findings suggest that hob3+ and BIN3 regulate F-actin localization, like RVS161, but that other roles for this gene have diverged somewhat during evolution.

The nucleotide sequences reported in this paper have been submitted to the GenBank^TM/EBI Data Bank with accession numbers AF2717232 (human BIN3 cDNA), AA418871 (human BIN3 EST), AAF76218 (human Bin3 protein), AF271733 (murine Bin3 cDNA), AF275638 (S. pombe hob3+ cDNA), and AAF86459 (S. pombe Hob3p).

The amino acid sequence alignment in Fig. 1 including the budding yeast protein Rvs161p has been submitted to the Swiss Protein Database under Swiss-Prot accession number 25343.

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