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Originally published In Press as doi:10.1074/jbc.M101877200 on April 3, 2001

J. Biol. Chem., Vol. 276, Issue 24, 21785-21790, June 15, 2001
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Overexpression of gamma -Sarcoglycan Induces Severe Muscular Dystrophy
IMPLICATIONS FOR THE REGULATION OF SARCOGLYCAN ASSEMBLY*

Xiaolei ZhuDagger §, Michele HadhazyDagger , Margaret E. GrohDagger , Matthew T. Wheeler, Robert Wollmann||, and Elizabeth M. McNallyDagger **

From the Dagger  Department of Medicine, Section of Cardiology, and Department of Human Genetics,  Department of Molecular Genetics and Cell Biology, and || Department of Pathology, The University of Chicago, Chicago, Illinois 60637

The sarcoglycan complex is found normally at the plasma membrane of muscle. Disruption of the sarcoglycan complex, through primary gene mutations in dystrophin or sarcoglycan subunits, produces membrane instability and muscular dystrophy. Restoration of the sarcoglycan complex at the plasma membrane requires reintroduction of the mutant sarcoglycan subunit in a manner that will permit normal assembly of the entire sarcoglycan complex. To study sarcoglycan gene replacement, we introduced transgenes expressing murine gamma -sarcoglycan into muscle of normal mice. Mice expressing high levels of gamma -sarcoglycan, under the control of the muscle-specific creatine kinase promoter, developed a severe muscular dystrophy with greatly reduced muscle mass and early lethality. Marked gamma -sarcoglycan overexpression produced cytoplasmic aggregates that interfered with normal membrane targeting of gamma -sarcoglycan. Overexpression of gamma -sarcoglycan lead to the up-regulation of alpha - and beta -sarcoglycan. These data suggest that increased gamma -sarcoglycan and/or mislocalization of gamma -sarcoglycan to the cytoplasm is sufficient to induce muscle damage and provides a new model of muscular dystrophy that highlights the importance of this protein in the assembly, function, and downstream signaling of the sarcoglycan complex. Most importantly, gene dosage and promoter strength should be given serious consideration in replacement gene therapy to ensure safety in human clinical trials.

* The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

§ Supported by National Research Service Award HL10288.

** Supported by Muscular Dystrophy Association, National Institutes of Health Grant HL61322; a Charles Culpeper Medical Scholar. To whom correspondence should be addressed: Section of Cardiology, The University of Chicago, 5841 S. Maryland, MC 6088, Chicago, IL 60637. Tel.: 773-702-2672; Fax: 773-702-2681; E-mail: emcnally@medicine.bsd.uchicago.edu.

Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.
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