Advertisement
JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M102150200 on April 6, 2001

J. Biol. Chem., Vol. 276, Issue 25, 22200-22208, June 22, 2001
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
276/25/22200    most recent
M102150200v1
Right arrow Submit a Letter to Editor
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowRequest Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Garner, B.
Right arrow Articles by Thillet, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Garner, B.
Right arrow Articles by Thillet, J.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Structural Elucidation of the N- and O-Glycans of Human Apolipoprotein(a)
ROLE OF O-GLYCANS IN CONFERRING PROTEASE RESISTANCE*

Brett GarnerDagger §, Anthony H. MerryDagger , Louise RoyleDagger , David J. HarveyDagger , Pauline M. RuddDagger , and Joëlle Thillet

From the Dagger  Oxford Glycobiology Institute, Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, United Kingdom and  INSERM, Unite 551, Hopital de la Pitie, 75651 Paris, France

Apolipoprotein(a) (apo(a)) is a multikringle domain glycoprotein that exists covalently linked to apolipoprotein B100 of low density lipoprotein, to form the lipoprotein(a) (Lp(a)) particle, or as proteolytic fragments. Elevated plasma concentrations of apo(a) and its fragments may promote atherosclerosis, but the underlying mechanisms are incompletely understood. The factors influencing apo(a) proteolysis are also uncertain. Here we have used exoglycosidase digestion and mass spectrometry to sequence the Asn (N)-linked and Ser/Thr (O)-linked oligosaccharides of human apo(a). We also assessed the potential role of apo(a) O-glycans in protecting thermolysin-sensitive regions of the polypeptide. Apo(a) contained two major N-glycans that accounted for 17% of the total oligosaccharide structures. The N-glycans were complex biantennary structures present in either a mono- or disialylated state. The O-glycans were mostly (80%) represented by the monosialylated core type 1 structure, NeuNAcalpha 2-3Galbeta 1-3GalNAc, with smaller amounts of disialylated and non-sialylated O-glycans also detected. Removal of apo(a) O-glycans by sialidase and O-glycosidase treatment dramatically increased the sensitivity of the polypeptide to thermolysin digestion. These studies provide the first direct sequencing data for apo(a) glycans and indicate a novel function for apo(a) O-glycans that is potentially related to the atherogenicity of Lp(a).

* This work was supported by a Wellcome Trust fellowship and Grant 058833 (to B. G.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

§ To whom correspondence should be addressed: Oxford Glycobiology Institute, Dept. of Biochemistry, University of Oxford, South Parks Rd., Oxford OX1 3QU, UK. Tel.: 44-1865-275780; Fax: 44-1865-275216; E-mail: brett@glycob.ox.ac.uk.

Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 GlycobiologyHome page
M. Wuhrer, C. A. M. Koeleman, J. M. Fitzpatrick, K. F. Hoffmann, A. M. Deelder, and C. H. Hokke
Gender-specific expression of complex-type N-glycans in schistosomes
Glycobiology, October 1, 2006; 16(10): 991 - 1006.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
E. N. Glaros, W. S. Kim, C. M. Quinn, J. Wong, I. Gelissen, W. Jessup, and B. Garner
Glycosphingolipid Accumulation Inhibits Cholesterol Efflux via the ABCA1/Apolipoprotein A-I Pathway: 1-PHENYL-2-DECANOYLAMINO-3-MORPHOLINO-1-PROPANOL IS A NOVEL CHOLESTEROL EFFLUX ACCELERATOR
J. Biol. Chem., July 1, 2005; 280(26): 24515 - 24523.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
M. R. Larsen, P. Hojrup, and P. Roepstorff
Characterization of Gel-separated Glycoproteins Using Two-step Proteolytic Digestion Combined with Sequential Microcolumns and Mass Spectrometry
Mol. Cell. Proteomics, February 1, 2005; 4(2): 107 - 119.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
S. M. Marcovina, M. L. Koschinsky, J. J. Albers, and S. Skarlatos
Report of the National Heart, Lung, and Blood Institute Workshop on Lipoprotein(a) and Cardiovascular Disease: Recent Advances and Future Directions
Clin. Chem., November 1, 2003; 49(11): 1785 - 1796.
[Abstract] [Full Text] [PDF]

Home page
 GlycobiologyHome page
K. G. Ten Hagen, T. A. Fritz, and L. A. Tabak
All in the family: the UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferases
Glycobiology, January 1, 2003; 13(1): 1R - 16R.
[Abstract] [Full Text] [PDF]

Home page
 GlycobiologyHome page
B. Garner, D. J. Harvey, L. Royle, M. Frischmann, F. Nigon, M. J. Chapman, and P. M. Rudd
Characterization of human apolipoprotein B100 oligosaccharides in LDL subfractions derived from normal and hyperlipidemic plasma: deficiency of {alpha}-N-acetylneuraminyllactosyl-ceramide in light and small dense LDL particles
Glycobiology, October 1, 2001; 11(10): 791 - 802.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2001 by the American Society for Biochemistry and Molecular Biology.
Advertisement
spacer
Advertisement
Advertisement