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J. Biol. Chem., Vol. 276, Issue 25, 22500-22506, June 22, 2001
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From the Laboratory of Applied Microbiology, Graduate School of
Bioresource and Bioenvironmental Sciences, Kyushu University, Hakozaki,
Fukuoka 812-8581, Japan
Biphenyl dioxygenase (Bph Dox) is
responsible for the initial dioxygenation of biphenyl. The large
subunit (BphA1) of Bph Dox plays a crucial role in determination of
substrate specificity of biphenyl-related compounds including
polychlorinated biphenyls (PCBs). Functional evolution of Bph Dox of
Pseudomonas pseudoalcaligenes KF707 was accomplished by
random priming recombination of the bphA1 gene, involving
two rounds of in vitro recombination and mutation followed
by selection for increased activity in vivo. Evolved Bph
Dox acquired novel and multifunctional degradation capabilities not
only for PCBs but also for dibenzofuran, dibenzo-p-dioxin, dibenzothiophene, and fluorene, the compounds scarcely attacked by the
original KF707 Bph Dox. The modes of oxygenation were angular and
lateral dioxygenation for dibenzofuran and
dibenzo-p-dioxin, sulfoxidation for dibenzothiophene, and
mono-oxygenation for fluorene. These enzymes also exhibited enhanced
degradation abilities for PCB congeners, retaining 2,3-dioxygenase
activity and gaining 3,4-dioxygenase activity, depending on the
chlorine substitution of PCB congeners. Further mutation analysis
revealed that the amino acid at position 376 in BphA1 is significantly
involved in the acquisition of multifunctional oxygenase
activities and mode of oxygenation.
Emergence of Multifunctional Oxygenase Activities by Random
Priming Recombination*
*
This work was supported in part by Core Research for
Evolutional Science and Technology of Japan Science and Technology
Corporation.The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
To whom correspondence should be addressed: Laboratory of Applied
Microbiology, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, Hakozaki 6-10-1, Fukuoka 812-8581, Japan.
Tel./Fax: 81-92-642-2849; E-mail: kfurukaw@agr.kyushu-u.ac.jp.
Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.
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