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Originally published In Press as doi:10.1074/jbc.M101234200 on April 5, 2001

J. Biol. Chem., Vol. 276, Issue 25, 22638-22647, June 22, 2001
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Functional Equivalence of Structurally Distinct Ribosomes in the Malaria Parasite, Plasmodium berghei*

Rosalina M. L. van SpaendonkDagger , Jai RamesarDagger , Auke van WigcherenDagger , Wijnand Eling§, Annette L. Beetsma§, Geert-Jan van Gemert§, Jo Hooghof§, Chris J. JanseDagger , and Andrew P. WatersDagger ||

From the Dagger  Department of Parasitology, Leiden University Medical Centre, Postbus 9600, 2300 RC Leiden, and the § Department of Medical Microbiology, Section Parasitology, University Hospital St. Radboud Nijmegen 6525 ED, The Netherlands

Unlike most eukaryotes, many apicomplexan parasites contain only a few unlinked copies of ribosomal RNA (rRNA) genes. Based on stage-specific expression of these genes and structural differences among the rRNA molecules it has been suggested that Plasmodium spp. produce functionally different ribosomes in different developmental stages. This hypothesis was investigated through comparison of the structure of the large subunit rRNA molecules of the rodent malaria parasite, Plasmodium berghei, and by disruption of both of the rRNA gene units that are transcribed exclusively during development of this parasite in the mosquito (S-type rRNA gene units). In contrast to the human parasite, Plasmodium falciparum, we did not find evidence of structural differences in core regions of the distinct large subunit rRNAs which are known to be associated with catalytic activity including the GTPase site that varies in P. falciparum. Knockout P. berghei parasites lacking either of the S-type gene units were able to complete development in both the vertebrate and mosquito hosts. These results formally exclude the hypothesis that two functionally different ribosome types distinct from the predominantly blood stage-expressed A-type ribosomes, are required for development of all Plasmodium species in the mosquito. The maintenance of two functionally equivalent rRNA genes might now be explained as a gene dosage phenomenon.

* This research was supported by the Research Council for Earth and Life Sciences, the Netherlands Organization for Scientific Research, and the INCO-DC program of the European Community Contract CT 960052.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

The nucleotide sequence(s) reported in this paper has been submitted to the GenBankTM/EMBL Data Bank with accession number(s) AJ301624, AJ298079, AJ298080, AJ298081, AJ298082. and AJ298083.

Present address: Division of Parasitology, Biomedical Primate Research Center, Rijswijk, The Netherlands.

|| To whom correspondence should be addressed. Tel.: 31-71-526-5069; Fax: 31-71-526-6907; E-mail: Waters@lumc.nl.

Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.
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