| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 276, Issue 25, 22901-22909, June 22, 2001
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
From the The initial step of the plastidic
2C-methyl-D-erythritol 4-phosphate (MEP) pathway that
produces isopentenyl diphosphate is catalyzed by
1-deoxy-D-xylulose-5-phosphate synthase. To investigate whether or not 1-deoxy-D-xylulose-5-phosphate synthase
catalyzes a limiting step in the MEP pathway in plants, we produced
transgenic Arabidopsis plants that over- or underexpress
this enzyme. Compared with non-transgenic wild-type plants, the
transgenic plants accumulate different levels of various isoprenoids
such as chlorophylls, tocopherols, carotenoids, abscisic acid, and
gibberellins. Phenotypically, the transgenic plants had slight
alterations in growth and germination rates. Because the levels of
several plastidic isoprenoids correlate with changes in
1-deoxy-D-xylulose-5-phosphate synthase levels, we conclude
that this enzyme catalyzes one of the rate-limiting steps of the MEP
biosynthetic pathway. Furthermore, since the product of the MEP pathway
is isopentenyl diphosphate, our results suggest that in plastids the
pool of isopentenyl diphosphate is limiting to isprenoid production.
1-Deoxy-D-xylulose-5-phosphate Synthase, a Limiting
Enzyme for Plastidic Isoprenoid Biosynthesis in Plants*
§,,, and
Departamento de Biología Molecular
de Plantas, Instituto de Biotecnología, Universidad Nacional
Autónoma de México, Avenida Universidad 2001 Chamilpa. Apartado Postal 510-3 Cuernavaca,
Morelos 62271, México and ¶ BASF AG. Crop Protection
Research, A30, 67056 Ludwigshafen, Germany
*
This research was supported by Consejo Nacional de
Ciencia y Tecnología Grant 31791-N, Dírección General
de Asuntos del Personal Academico Grant IN205697, and by BASF.The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
To whom correspondence should be addressed. Tel.:
52-5-6227856; Fax: 52-73-139988; E-mail: patricia@ibt.unam.mx.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  C. Tretner, U. Huth, and B. Hause Mechanostimulation of Medicago truncatula leads to enhanced levels of jasmonic acid J. Exp. Bot., July 1, 2008; 59(10): 2847 - 2856. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 U. Flores-Perez, S. Sauret-Gueto, E. Gas, P. Jarvis, and M. Rodriguez-Concepcion A Mutant Impaired in the Production of Plastome-Encoded Proteins Uncovers a Mechanism for the Homeostasis of Isoprenoid Biosynthetic Enzymes in Arabidopsis Plastids PLANT CELL, May 1, 2008; 20(5): 1303 - 1315. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Okada, H. Kasahara, S. Yamaguchi, H. Kawaide, Y. Kamiya, H. Nojiri, and H. Yamane Genetic Evidence for the Role of Isopentenyl Diphosphate Isomerases in the Mevalonate Pathway and Plant Development in Arabidopsis Plant Cell Physiol., April 1, 2008; 49(4): 604 - 616. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. D. Sharkey, A. E. Wiberley, and A. R. Donohue Isoprene Emission from Plants: Why and How Ann. Bot., January 1, 2008; 101(1): 5 - 18. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Andre and C. Benning Arabidopsis Seedlings Deficient in a Plastidic Pyruvate Kinase Are Unable to Utilize Seed Storage Compounds for Germination and Establishment Plant Physiology, December 1, 2007; 145(4): 1670 - 1680. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Loivamaki, F. Gilmer, R. J. Fischbach, C. Sorgel, A. Bachl, A. Walter, and J.-P. Schnitzler Arabidopsis, a Model to Study Biological Functions of Isoprene Emission? Plant Physiology, June 1, 2007; 144(2): 1066 - 1078. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Xiang, G. Usunow, G. Lange, M. Busch, and L. Tong Crystal Structure of 1-Deoxy-D-xylulose 5-Phosphate Synthase, a Crucial Enzyme for Isoprenoids Biosynthesis J. Biol. Chem., January 26, 2007; 282(4): 2676 - 2682. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Loivamaki, S. Louis, G. Cinege, I. Zimmer, R. J. Fischbach, and J.-P. Schnitzler Circadian Rhythms of Isoprene Biosynthesis in Grey Poplar Leaves Plant Physiology, January 1, 2007; 143(1): 540 - 551. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. L. Morris, L. J. M. Ducreux, P. Hedden, S. Millam, and M. A. Taylor Overexpression of a bacterial 1-deoxy-D-xylulose 5-phosphate synthase gene in potato tubers perturbs the isoprenoid metabolic network: implications for the control of the tuber life cycle J. Exp. Bot., September 1, 2006; 57(12): 3007 - 3018. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Sauret-Gueto, P. Botella-Pavia, U. Flores-Perez, J. F. Martinez-Garcia, C. San Roman, P. Leon, A. Boronat, and M. Rodriguez-Concepcion Plastid Cues Posttranscriptionally Regulate the Accumulation of Key Enzymes of the Methylerythritol Phosphate Pathway in Arabidopsis Plant Physiology, May 1, 2006; 141(1): 75 - 84. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Tambasco-Studart, O. Titiz, T. Raschle, G. Forster, N. Amrhein, and T. B. Fitzpatrick Vitamin B6 biosynthesis in higher plants PNAS, September 20, 2005; 102(38): 13687 - 13692. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Mayrhofer, M. Teuber, I. Zimmer, S. Louis, R. J. Fischbach, and J.-P. Schnitzler Diurnal and Seasonal Variation of Isoprene Biosynthesis-Related Genes in Grey Poplar Leaves Plant Physiology, September 1, 2005; 139(1): 474 - 484. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M.-H. Hsieh and H. M. Goodman The Arabidopsis IspH Homolog Is Involved in the Plastid Nonmevalonate Pathway of Isoprenoid Biosynthesis Plant Physiology, June 1, 2005; 138(2): 641 - 653. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Guevara-Garcia, C. San Roman, A. Arroyo, M. E. Cortes, M. de la Luz Gutierrez-Nava, and P. Leon Characterization of the Arabidopsis clb6 Mutant Illustrates the Importance of Posttranscriptional Regulation of the Methyl-D-Erythritol 4-Phosphate Pathway PLANT CELL, February 1, 2005; 17(2): 628 - 643. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Dudareva, S. Andersson, I. Orlova, N. Gatto, M. Reichelt, D. Rhodes, W. Boland, and J. Gershenzon From The Cover: The nonmevalonate pathway supports both monoterpene and sesquiterpene formation in snapdragon flowers PNAS, January 18, 2005; 102(3): 933 - 938. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Jennewein, M. R. Wildung, M. Chau, K. Walker, and R. Croteau Random sequencing of an induced Taxus cell cDNA library for identification of clones involved in Taxol biosynthesis PNAS, June 15, 2004; 101(24): 9149 - 9154. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Collakova and D. DellaPenna The Role of Homogentisate Phytyltransferase and Other Tocopherol Pathway Enzymes in the Regulation of Tocopherol Synthesis during Abiotic Stress Plant Physiology, October 1, 2003; 133(2): 930 - 940. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. O. Lindgren, K. G. Stalberg, and A.-S. Hoglund Seed-Specific Overexpression of an Endogenous Arabidopsis Phytoene Synthase Gene Results in Delayed Germination and Increased Levels of Carotenoids, Chlorophyll, and Abscisic Acid Plant Physiology, June 1, 2003; 132(2): 779 - 785. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Collakova and D. DellaPenna Homogentisate Phytyltransferase Activity Is Limiting for Tocopherol Biosynthesis in Arabidopsis Plant Physiology, February 1, 2003; 131(2): 632 - 642. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Kasahara, A. Hanada, T. Kuzuyama, M. Takagi, Y. Kamiya, and S. Yamaguchi Contribution of the Mevalonate and Methylerythritol Phosphate Pathways to the Biosynthesis of Gibberellins in Arabidopsis J. Biol. Chem., November 15, 2002; 277(47): 45188 - 45194. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Carretero-Paulet, I. Ahumada, N. Cunillera, M. Rodriguez-Concepcion, A. Ferrer, A. Boronat, and N. Campos Expression and Molecular Analysis of the Arabidopsis DXR Gene Encoding 1-Deoxy-D-Xylulose 5-Phosphate Reductoisomerase, the First Committed Enzyme of the 2-C-Methyl-D-Erythritol 4-Phosphate Pathway Plant Physiology, August 1, 2002; 129(4): 1581 - 1591. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. N. Rosenstiel, A. J. Fisher, R. Fall, and R. K. Monson Differential Accumulation of Dimethylallyl Diphosphate in Leaves and Needles of Isoprene- and Methylbutenol-Emitting and Nonemitting Species Plant Physiology, July 1, 2002; 129(3): 1276 - 1284. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. R. Finkelstein, S. S. L. Gampala, and C. D. Rock Abscisic Acid Signaling in Seeds and Seedlings PLANT CELL, May 1, 2002; 14(90001): S15 - 45. [Full Text] [PDF]
|
|
|
|
|
|
P. Broun and C. Somerville Progress in plant metabolic engineering PNAS, July 31, 2001; 98(16): 8925 - 8927. [Full Text] [PDF]
|
|
|
|
|
|
S. B. Richard, J.-L. Ferrer, M. E. Bowman, A. M. Lillo, C. N. Tetzlaff, D. E. Cane, and J. P. Noel Structure and Mechanism of 2-C-Methyl-D-erythritol 2,4-Cyclodiphosphate Synthase. AN ENZYME IN THE MEVALONATE-INDEPENDENT ISOPRENOID BIOSYNTHETIC PATHWAY J. Biol. Chem., March 1, 2002; 277(10): 8667 - 8672. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
