|
Originally published In Press as doi:10.1074/jbc.M101288200 on April 9, 2001
J. Biol. Chem., Vol. 276, Issue 25, 23084-23091, June 22, 2001
A Novel Plasmal Conjugate to Glycerol and Psychosine
("Glyceroplasmalopsychosine")
ISOLATION AND CHARACTERIZATION FROM BOVINE BRAIN WHITE
MATTER*
Toshiyuki
Hikita §,
Keiko
Tadano-Aritomi¶,
Naoko
Iida-Tanaka¶,
Jasbir K.
Anand ,
Ineo
Ishizuka¶, and
Sen-itiroh
Hakomori §
From the Division of Biomembrane Research, Pacific
Northwest Research Institute, Seattle, Washington 98122-4327, the
§ Department of Pathobiology, University of Washington,
Seattle, Washington 98195-7238, and the ¶ Department of
Biochemistry, Teikyo University School of Medicine, Tokyo 173-8605, Japan
A novel plasmal conjugate of
glycosphingolipid having cationic lipid properties was isolated from
the white matter of bovine brain. Linkage analysis of galactosyl
residue by methylation, liquid secondary ion, and electrospray
ionization mass spectrometry of intact and methylated derivatives, and
by 1H- and 13C-NMR spectroscopy, identified the
structure unambiguously as an O-acetal conjugate of plasmal
to the primary hydroxyl group of glycerol and to the 6-hydroxyl group
of galactosyl residue of -galactosyl 1 1 sphingosine (psychosine).
This novel compound is hereby termed
"glyceroplasmalopsychosine"; its structure is shown
below.
*
This study was supported by National Institutes of Health
Grant NS39560 (to S. H.).The costs of publication of this
article were defrayed in part by the
payment of page charges. The article must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
To whom correspondence should be addressed: Dept. of
Pathobiology, University of Washington, Seattle, WA 98195-7238. Tel.: 206-726-1222; Fax: 206-726-1212; E-mail:
hakomori@u.washington.edu.
Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.

CiteULike Complore Connotea Del.icio.us Digg Reddit Technorati What's this?
This article has been cited by other articles:

|
 |

|
 |
 
J. Matsuda, M. Kido, K. Tadano-Aritomi, I. Ishizuka, K. Tominaga, K. Toida, E. Takeda, K. Suzuki, and Y. Kuroda
Mutation in saposin D domain of sphingolipid activator protein gene causes urinary system defects and cerebellar Purkinje cell degeneration with accumulation of hydroxy fatty acid-containing ceramide in mouse
Hum. Mol. Genet.,
November 1, 2004;
13(21):
2709 - 2723.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
K. Tadano-Aritomi, J. Matsuda, H. Fujimoto, K. Suzuki, and I. Ishizuka
Seminolipid and its precursor/degradative product, galactosylalkylacylglycerol, in the testis of saposin A- and prosaposin-deficient mice
J. Lipid Res.,
September 1, 2003;
44(9):
1737 - 1743.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
J. Bodennec, D. Pelled, and A. H. Futerman
Aminopropyl solid phase extraction and 2 D TLC of neutral glycosphingolipids and neutral lysoglycosphingolipids
J. Lipid Res.,
January 1, 2003;
44(1):
218 - 226.
[Abstract]
[Full Text]
[PDF]
|
 |
|
Copyright © 2001 by the American Society for Biochemistry and Molecular Biology.
|
Advertisement
Advertisement
|