|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 276, Issue 26, 23413-23420, June 29, 2001
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
From the Aquaporins (AQPs) are channel proteins
that regulate the movement of water through the plasma membrane of
secretory and absorptive cells in response to osmotic gradients. In the
salivary gland, AQP5 is the major aquaporin expressed on the apical
membrane of acinar cells. Previous studies have shown that the volume
of saliva secreted by AQP5-deficient mice is decreased, indicating a
role for AQP5 in saliva secretion; however, the mechanism by which AQP5
regulates water transport in salivary acinar cells remains to be
determined. Here we show that the decreased salivary flow rate and
increased tonicity of the saliva secreted by
Aqp5
Salivary Acinar Cells from Aquaporin 5-deficient Mice Have
Decreased Membrane Water Permeability and Altered Cell Volume
Regulation*
,¶,, and
Department of Molecular Genetics,
Biochemistry, and Microbiology, University of Cincinnati College of
Medicine, Cincinnati, Ohio 45267-0524 and the § Center for
Oral Biology, University of Rochester School of Medicine and Dentistry,
Rochester, New York 14642
/ mice in response to
pilocarpine stimulation are not caused by changes in whole body fluid
homeostasis, indicated by similar blood gas and electrolyte
concentrations in urine and blood in wild-type and AQP5-deficient mice.
In contrast, the water permeability in parotid and sublingual acinar
cells isolated from Aqp5/ mice
is decreased significantly. Water permeability decreased by 65% in
parotid and 77% in sublingual acinar cells from
Aqp5/ mice in response to
hypertonicity-induced cell shrinkage and hypotonicity-induced cell
swelling. These data show that AQP5 is the major pathway for regulating
the water permeability in acinar cells, a critical property of the
plasma membrane which determines the flow rate and ionic composition of
secreted saliva.
*
This work was supported in part by National Institutes of
Health Grants RO1 DE138283 and ES06096 (to A. G. M.), RO1
DEO8921 (to J. E. M.), NHLBI, National Institutes of Health, Program
of Excellence in Molecular Biology of Heart and Lung Grant HL61781 (to
A. G. M.) and for new investigator support from this program (to
C. M. K.).The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
To whom correspondence should be addressed: Dept. of Molecular
Genetics, Biochemistry and Microbiology, University of Cincinnati College of Medicine, 231 Bethesda Ave., 3110 MSB, P. O. Box 670524, Cincinnati, OH 45267-0524. Tel.: 513-558-5534; Fax:
513-558-1885; E-mail: Anil.Menon@UC.edu.
Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  U. Hasler Controlled aquaporin-2 expression in the hypertonic environment Am J Physiol Cell Physiol, April 1, 2009; 296(4): C641 - C653. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C.-H. Yeung, C. Callies, A. Rojek, S. Nielsen, and T. G. Cooper Aquaporin Isoforms Involved in Physiological Volume Regulation of Murine Spermatozoa Biol Reprod, February 1, 2009; 80(2): 350 - 357. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Heitzmann and R. Warth Physiology and Pathophysiology of Potassium Channels in Gastrointestinal Epithelia Physiol Rev, July 1, 2008; 88(3): 1119 - 1182. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. J. Watson, I. Kim, A. F. Baquero, C. A. Burks, L. Liu, and T. A. Gilbertson Expression of Aquaporin Water Channels in Rat Taste Buds Chem Senses, June 1, 2007; 32(5): 411 - 421. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Nakamoto, A. Srivastava, V. G. Romanenko, C. E. Ovitt, P. Perez-Cornejo, J. Arreola, T. Begenisich, and J. E. Melvin Functional and molecular characterization of the fluid secretion mechanism in human parotid acinar cells Am J Physiol Regulatory Integrative Comp Physiol, June 1, 2007; 292(6): R2380 - R2390. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Tamma, G. Procino, A. Strafino, E. Bononi, G. Meyer, M. Paulmichl, V. Formoso, M. Svelto, and G. Valenti Hypotonicity Induces Aquaporin-2 Internalization and Cytosol-to-Membrane Translocation of ICln in Renal Cells Endocrinology, March 1, 2007; 148(3): 1118 - 1130. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. D. Kawedia, M. L. Nieman, G. P. Boivin, J. E. Melvin, K.-I. Kikuchi, A. R. Hand, J. N. Lorenz, and A. G. Menon Interaction between transcellular and paracellular water transport pathways through Aquaporin 5 and the tight junction complex PNAS, February 27, 2007; 104(9): 3621 - 3626. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H.-J. Lee, Y.-J. Lee, H.-C. Kwon, S. Bae, S.-H. Kim, J.-J. Min, C.-K. Cho, and Y.-S. Lee Radioprotective Effect of Heat Shock Protein 25 on Submandibular Glands of Rats Am. J. Pathol., November 1, 2006; 169(5): 1601 - 1611. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Romanenko, T. Nakamoto, A. Srivastava, J. E. Melvin, and T. Begenisich Molecular Identification and Physiological Roles of Parotid Acinar Cell Maxi-K Channels J. Biol. Chem., September 22, 2006; 281(38): 27964 - 27972. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. J. Dawson, P. C. Fox, and P. M. Smith Sjogrens syndrome--the non-apoptotic model of glandular hypofunction Rheumatology, July 1, 2006; 45(7): 792 - 798. [Full Text] [PDF]
|
|
|
|
|
|
X. Liu, B. Bandyopadhyay, T. Nakamoto, B. Singh, W. Liedtke, J. E. Melvin, and I. Ambudkar A Role for AQP5 in Activation of TRPV4 by Hypotonicity: CONCERTED INVOLVEMENT OF AQP5 AND TRPV4 IN REGULATION OF CELL VOLUME RECOVERY J. Biol. Chem., June 2, 2006; 281(22): 15485 - 15495. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. K. Sidhaye, A. D. Guler, K. S. Schweitzer, F. D'Alessio, M. J. Caterina, and L. S. King Transient receptor potential vanilloid 4 regulates aquaporin-5 abundance under hypotonic conditions PNAS, March 21, 2006; 103(12): 4747 - 4752. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Futatsugi, T. Nakamura, M. K. Yamada, E. Ebisui, K. Nakamura, K. Uchida, T. Kitaguchi, H. Takahashi-Iwanaga, T. Noda, J. Aruga, et al. IP3 Receptor Types 2 and 3 Mediate Exocrine Secretion Underlying Energy Metabolism Science, September 30, 2005; 309(5744): 2232 - 2234. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. S. Verkman More than just water channels: unexpected cellular roles of aquaporins J. Cell Sci., August 1, 2005; 118(15): 3225 - 3232. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Yang, Y. Song, D. Zhao, and A. S. Verkman Phenotype analysis of aquaporin-8 null mice Am J Physiol Cell Physiol, May 1, 2005; 288(5): C1161 - C1170. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Sidhaye, J. D. Hoffert, and L. S. King cAMP Has Distinct Acute and Chronic Effects on Aquaporin-5 in Lung Epithelial Cells J. Biol. Chem., February 4, 2005; 280(5): 3590 - 3596. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Begenisich, T. Nakamoto, C. E. Ovitt, K. Nehrke, C. Brugnara, S. L. Alper, and J. E. Melvin Physiological Roles of the Intermediate Conductance, Ca2+-activated Potassium Channel Kcnn4 J. Biol. Chem., November 12, 2004; 279(46): 47681 - 47687. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Nakamura, M. Matsui, K. Uchida, A. Futatsugi, S. Kusakawa, N. Matsumoto, K. Nakamura, T. Manabe, M. M. Taketo, and K. Mikoshiba M3 muscarinic acetylcholine receptor plays a critical role in parasympathetic control of salivation in mice J. Physiol., July 15, 2004; 558(2): 561 - 575. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Gresz, T.-H. Kwon, H. Gong, P. Agre, M. C. Steward, L. S. King, and S. Nielsen Immunolocalization of AQP-5 in rat parotid and submandibular salivary glands after stimulation or inhibition of secretion in vivo Am J Physiol Gastrointest Liver Physiol, July 1, 2004; 287(1): G151 - G161. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Yang, J. D. Kawedia, and A. G. Menon Cyclic AMP Regulates Aquaporin 5 Expression at Both Transcriptional and Post-transcriptional Levels through a Protein Kinase A Pathway J. Biol. Chem., August 22, 2003; 278(34): 32173 - 32180. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Inoue, H. Iida, Z. Yuan, Y. Ishikawa, and H. Ishida Age-related Decreases in the Response of Aquaporin-5 to Acetylcholine in Rat Parotid Glands Journal of Dental Research, June 1, 2003; 82(6): 476 - 480. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Javot, V. Lauvergeat, V. Santoni, F. Martin-Laurent, J. Guclu, J. Vinh, J. Heyes, K. I. Franck, A. R. Schaffner, D. Bouchez, et al. Role of a Single Aquaporin Isoform in Root Water Uptake PLANT CELL, February 1, 2003; 15(2): 509 - 522. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Agre, L. S King, M. Yasui, W. B Guggino, O. P. Ottersen, Y. Fujiyoshi, A. Engel, and S. Nielsen Aquaporin water channels - from atomic structure to clinical medicine J. Physiol., July 1, 2002; 542(1): 3 - 16. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Nehrke, J. Arreola, H.-V. Nguyen, J. Pilato, L. Richardson, G. Okunade, R. Baggs, G. E. Shull, and J. E. Melvin Loss of Hyperpolarization-activated Cl- Current in Salivary Acinar Cells from Clcn2 Knockout Mice J. Biol. Chem., June 21, 2002; 277(26): 23604 - 23611. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Song, N. Sonawane, and A S Verkman Localization of aquaporin-5 in sweat glands and functional analysis using knockout mice J. Physiol., June 1, 2002; 541(2): 561 - 568. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Ishikawa, H. Iida, and H. Ishida The Muscarinic Acetylcholine Receptor-Stimulated Increase in Aquaporin-5 Levels in the Apical Plasma Membrane in Rat Parotid Acinar Cells Is Coupled with Activation of Nitric Oxide/cGMP Signal Transduction Mol. Pharmacol., June 1, 2002; 61(6): 1423 - 1434. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. N. Nejsum, T.-H. Kwon, U. B. Jensen, O. Fumagalli, J. Frokiaer, C. M. Krane, A. G. Menon, L. S. King, P. C. Agre, and S. Nielsen Functional requirement of aquaporin-5 in plasma membranes of sweat glands PNAS, January 1, 2002; (2002) 12588099. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. M. Krane, C. N. Fortner, A. R. Hand, D. W. McGraw, J. N. Lorenz, S. E. Wert, J. E. Towne, R. J. Paul, J. A. Whitsett, and A. G. Menon Aquaporin 5-deficient mouse lungs are hyperresponsive to cholinergic stimulation PNAS, November 9, 2001; (2001) 231273398. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. N. Nejsum, T.-H. Kwon, U. B. Jensen, O. Fumagalli, J. Frokiaer, C. M. Krane, A. G. Menon, L. S. King, P. C. Agre, and S. Nielsen Functional requirement of aquaporin-5 in plasma membranes of sweat glands PNAS, January 8, 2002; 99(1): 511 - 516. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. M. Krane, C. N. Fortner, A. R. Hand, D. W. McGraw, J. N. Lorenz, S. E. Wert, J. E. Towne, R. J. Paul, J. A. Whitsett, and A. G. Menon Aquaporin 5-deficient mouse lungs are hyperresponsive to cholinergic stimulation PNAS, November 20, 2001; 98(24): 14114 - 14119. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |