Advertisement
JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M101034200 on April 20, 2001

J. Biol. Chem., Vol. 276, Issue 26, 23607-23615, June 29, 2001
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
276/26/23607    most recent
M101034200v1
Right arrow Submit a Letter to Editor
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowRequest Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Mukherjee, K.
Right arrow Articles by Mukhopadhyay, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Mukherjee, K.
Right arrow Articles by Mukhopadhyay, A.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

SopE Acts as an Rab5-specific Nucleotide Exchange Factor and Recruits Non-prenylated Rab5 on Salmonella-containing Phagosomes to Promote Fusion with Early Endosomes*

Konark MukherjeeDagger , Seetharaman ParashuramanDagger , Manoj Raje§, and Amitabha MukhopadhyayDagger

From the Dagger  National Institute of Immunology, Aruna Asaf Ali Marg, New Delhi 110067 and the § Institute of Microbial Technology, Sector 39A, Chandigarh 160036, India

Rab-GTPase regulates the fusion between two specific vesicles. It is well documented that, for their biological function, Rab proteins need to be prenylated for attachment to the vesicle membrane. In contrast, we showed in the present investigation that SopE, a type III secretory protein of Salmonella, translocates onto Salmonella-containing phagosomes (LSP) and mediates the recruitment of non-prenylated Rab5 (Rab5:Delta C4) on LSP in GTP form. Simultaneously, SopE present in infected cell cytosol acts as an Rab5-specific exchange factor and converts the inactive Rab-GDP to the GTP form. The non-prenylated Rab5 subsequently promoted efficient fusion of LSP with early endosomes. This is the first demonstration that a prenylation-deficient Rab protein retains biological activity and can promote vesicle fusion, if it is recruited on the membrane by some other method.

* These studies are supported by grants from the Department of Biotechnology and Indian Council of Medical Research.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

To whom correspondence should be addressed: Cell Biology Laboratory, National Institute of Immunology, New Delhi 110067, India. Tel.: 91-11-6162281 or 91-11-6183004; Fax: 91-11-6109433 or 91-11-6162125; E-mail: amitabha@nii.res.in.

Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Virol.Home page
M. Stone, S. Jia, W. D. Heo, T. Meyer, and K. V. Konan
Participation of Rab5, an Early Endosome Protein, in Hepatitis C Virus RNA Replication Machinery
J. Virol., May 1, 2007; 81(9): 4551 - 4563.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
Séb. P. Faucher, S. Porwollik, C. M. Dozois, M. McClelland, and F. Daigle
Transcriptome of Salmonella enterica serovar Typhi within macrophages revealed through the selective capture of transcribed sequences
PNAS, February 7, 2006; 103(6): 1906 - 1911.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
G. Krishnamurthy, R. Vikram, S. B. Singh, N. Patel, S. Agarwal, G. Mukhopadhyay, S. K. Basu, and A. Mukhopadhyay
Hemoglobin Receptor in Leishmania Is a Hexokinase Located in the Flagellar Pocket
J. Biol. Chem., February 18, 2005; 280(7): 5884 - 5891.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
M. Marsman, I. Jordens, C. Kuijl, L. Janssen, and J. Neefjes
Dynein-mediated Vesicle Transport Controls Intracellular Salmonella Replication
Mol. Biol. Cell, June 1, 2004; 15(6): 2954 - 2964.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
M. P. Stevens, A. Friebel, L. A. Taylor, M. W. Wood, P. J. Brown, W.-D. Hardt, and E. E. Galyov
A Burkholderia pseudomallei Type III Secreted Protein, BopE, Facilitates Bacterial Invasion of Epithelial Cells and Exhibits Guanine Nucleotide Exchange Factor Activity
J. Bacteriol., August 15, 2003; 185(16): 4992 - 4996.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
K. Mukherjee, S. Parashuraman, G. Krishnamurthy, J. Majumdar, A. Yadav, R. Kumar, S. K. Basu, and A. Mukhopadhyay
Diverting intracellular trafficking of Salmonella to the lysosome through activation of the late endocytic Rab7 by intracellular delivery of muramyl dipeptide
J. Cell Sci., September 15, 2002; 115(18): 3693 - 3701.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
N. Perskvist, K. Roberg, A. Kulyte, and O. Stendahl
Rab5a GTPase regulates fusion between pathogen-containing phagosomes and cytoplasmic organelles in human neutrophils
J. Cell Sci., March 15, 2002; 115(6): 1321 - 1330.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. K. Criss, M. Silva, J. E. Casanova, and B. A. McCormick
Regulation of Salmonella-induced Neutrophil Transmigration by Epithelial ADP-ribosylation Factor 6
J. Biol. Chem., December 14, 2001; 276(51): 48431 - 48439.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2001 by the American Society for Biochemistry and Molecular Biology.
Advertisement
spacer
Advertisement
Advertisement