|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 276, Issue 26, 24223-24231, June 29, 2001
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
From the Department of Microbiology and Immunology, Stanford
University, Stanford, California 94305-5124
Cells infected with the intracellular protozoan
parasite Toxoplasma gondii undergo up-regulation of
pro-inflammatory cytokines, organelle redistribution, and protection
from apoptosis. To examine the molecular basis of these and other
changes, gene expression profiles of human foreskin fibroblasts
infected with Toxoplasma were studied using human cDNA
microarrays consisting of ~22,000 known genes and uncharacterized
expressed sequence tags. Early during infection (1-2 h), <1%
of all genes show a significant change in the abundance of their
transcripts. Of the 63 known genes in this group, 27 encode proteins
associated with the immune response. These genes are also up-regulated
by secreted, soluble factors from extracellular parasites indicating
that the early response does not require parasite invasion. Later
during infection, genes involved in numerous host cell processes,
including glucose and mevalonate metabolism, are modulated. Many of
these late genes are dependent on the direct presence of the parasite;
i.e. secreted products from either the parasite or infected
cells are insufficient to induce these changes. These results reveal
several previously unknown effects on the host cell and lay the
foundation for detailed analysis of their role in the host-pathogen interaction.
Microarray Analysis Reveals Previously Unknown
Changes in Toxoplasma gondii-infected Human Cells*
,
*
The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
Supported by National Institutes of Health Grant 1F32AI10478-01.
§
Current address: Mycometrix, 213 E. Grand Ave., South San
Francisco, CA 94080.
¶
Supported by National Institutes of Health Grants AI21423 and
AI45057. To whom correspondence should be addressed: Dept. of Microbiology and Immunology, Stanford University, 299 Campus Dr., Stanford, CA 94305-5124. Tel.: 650-723-7984; Fax: 650-723-6853; E-mail:
john.boothroyd@Stanford.edu.
Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  E. D. Phelps, K. R. Sweeney, and I. J. Blader Toxoplasma gondii Rhoptry Discharge Correlates with Activation of the Early Growth Response 2 Host Cell Transcription Factor Infect. Immun., October 1, 2008; 76(10): 4703 - 4712. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. M. Nelson, A. R. Jones, J. C. Carmen, A. P. Sinai, R. Burchmore, and J. M. Wastling Modulation of the Host Cell Proteome by the Intracellular Apicomplexan Parasite Toxoplasma gondii Infect. Immun., February 1, 2008; 76(2): 828 - 844. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. B. Carruthers and Y. Suzuki Effects of Toxoplasma gondii Infection on the Brain Schizophr Bull, May 1, 2007; 33(3): 745 - 751. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. E. Fouts and J. C. Boothroyd Infection with Toxoplasma gondii Bradyzoites Has a Diminished Impact on Host Transcript Levels Relative to Tachyzoite Infection Infect. Immun., February 1, 2007; 75(2): 634 - 642. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Taylor, A. Barragan, C. Su, B. Fux, S. J. Fentress, K. Tang, W. L. Beatty, H. E. Hajj, M. Jerome, M. S. Behnke, et al. A Secreted Serine-Threonine Kinase Determines Virulence in the Eukaryotic Pathogen Toxoplasma gondii Science, December 15, 2006; 314(5806): 1776 - 1780. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Andersson, B. Hartmanova, P. Ryden, L. Noppa, L. Naslund, and A. Sjostedt A microarray analysis of the murine macrophage response to infection with Francisella tularensis LVS. J. Med. Microbiol., August 1, 2006; 55(Pt 8): 1023 - 1033. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Okomo-Adhiambo, C. Beattie, and A. Rink cDNA Microarray Analysis of Host-Pathogen Interactions in a Porcine In Vitro Model for Toxoplasma gondii Infection Infect. Immun., July 1, 2006; 74(7): 4254 - 4265. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Fahling, R. Mrowka, A. Steege, P. Martinka, P. B. Persson, and B. J. Thiele Heterogeneous Nuclear Ribonucleoprotein-A2/B1 Modulate Collagen Prolyl 4-Hydroxylase, {alpha} (I) mRNA Stability J. Biol. Chem., April 7, 2006; 281(14): 9279 - 9286. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. E. Molestina and A. P. Sinai Host and parasite-derived IKK activities direct distinct temporal phases of NF-{kappa}B activation and target gene expression following Toxoplasma gondii infection J. Cell Sci., December 15, 2005; 118(24): 5785 - 5796. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. W. Zhou, B. F. C. Kafsack, R. N. Cole, P. Beckett, R. F. Shen, and V. B. Carruthers The Opportunistic Pathogen Toxoplasma gondii Deploys a Diverse Legion of Invasion and Survival Proteins J. Biol. Chem., October 7, 2005; 280(40): 34233 - 34244. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. UNNIKRISHNAN and B. A. BURLEIGH Inhibition of host connective tissue growth factor expression: a novel Trypanosoma cruzi-mediated response FASEB J, November 1, 2004; 18(14): 1625 - 1635. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. N. Ledger, P. Pinton, D. Bourges, P. Roumi, H. Salmon, and I. P. Oswald Development of a Macroarray To Specifically Analyze Immunological Gene Expression in Swine Clin. Vaccine Immunol., July 1, 2004; 11(4): 691 - 698. [Abstract] [Full Text]
|
|
|
|
|
|
L. R. Portugal, L. R. Fernandes, G. C. Cesar, H. C. Santiago, D. R. Oliveira, N. M. Silva, A. A. Silva, J. Lannes-Vieira, R. M. E. Arantes, R. T. Gazzinelli, et al. Infection with Toxoplasma gondii Increases Atherosclerotic Lesion in ApoE-Deficient Mice Infect. Immun., June 1, 2004; 72(6): 3571 - 3576. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Zhao, M. L. Whitfield, T. Xu, D. Botstein, and J. D. Brooks Diverse Effects of Methylseleninic Acid on the Transcriptional Program of Human Prostate Cancer Cells Mol. Biol. Cell, February 1, 2004; 15(2): 506 - 519. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. E. Molestina, T. M. Payne, I. Coppens, and A. P. Sinai Activation of NF-{kappa}B by Toxoplasma gondii correlates with increased expression of antiapoptotic genes and localization of phosphorylated I{kappa}B to the parasitophorous vacuole membrane J. Cell Sci., November 1, 2003; 116(21): 4359 - 4371. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. M. Payne, R. E. Molestina, and A. P. Sinai Inhibition of caspase activation and a requirement for NF-{kappa}B function in the Toxoplasma gondii-mediated blockade of host apoptosis J. Cell Sci., November 1, 2003; 116(21): 4345 - 4358. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Lin, M. T. Vahey, D. Thach, D. A. Stenger, and J. J. Pancrazio Biological Threat Detection via Host Gene Expression Profiling Clin. Chem., July 1, 2003; 49(7): 1045 - 1049. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Natarajan, M. S. Rajala, and J. Chodosh Corneal IL-8 Expression Following Adenovirus Infection Is Mediated by c-Src Activation in Human Corneal Fibroblasts J. Immunol., June 15, 2003; 170(12): 6234 - 6243. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. B. van 't Wout, G. K. Lehrman, S. A. Mikheeva, G. C. O'Keeffe, M. G. Katze, R. E. Bumgarner, G. K. Geiss, and J. I. Mullins Cellular Gene Expression upon Human Immunodeficiency Virus Type 1 Infection of CD4+-T-Cell Lines J. Virol., December 20, 2002; 77(2): 1392 - 1402. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. S. Mocarski Jr. Virus self-improvement through inflammation: No pain, no gain PNAS, March 19, 2002; 99(6): 3362 - 3364. [Full Text] [PDF]
|
|
|
|
|
|
S. V. de Avalos, I. J. Blader, M. Fisher, J. C. Boothroyd, and B. A. Burleigh Immediate/Early Response to Trypanosoma cruzi Infection Involves Minimal Modulation of Host Cell Transcription J. Biol. Chem., January 4, 2002; 277(1): 639 - 644. [Abstract] [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |