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J. Biol. Chem., Vol. 276, Issue 26, 24253-24260, June 29, 2001
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From the Striatin and S/G2 nuclear
autoantigen (SG2NA) are related proteins that contain membrane binding
domains and associate with protein phosphatase 2A (PP2A) and many
additional proteins that may be PP2A regulatory targets. Here we
identify a major member of these complexes as class II mMOB1, a
mammalian homolog of the yeast protein MOB1, and show that its
phosphorylation appears to be regulated by PP2A. Yeast MOB1 is critical
for cytoskeletal reorganization during cytokinesis and exit from
mitosis. We show that mMOB1 associated with PP2A is not detectably
phosphorylated in asynchronous murine fibroblasts. However, treatment
with the PP2A inhibitor okadaic acid induces phosphorylation of
PP2A-associated mMOB1 on serine. Moreover, specific inhibition of PP2A
also results in hyperphosphorylation of striatin, SG2NA, and three
unidentified proteins, suggesting that these proteins may also be
regulated by PP2A. Indirect immunofluorescence produced highly similar
staining patterns for striatin, SG2NA, and mMOB1, with the highest
concentrations for each protein adjacent to the nuclear membrane. We
also present evidence that these complexes may interact with each
other. These data are consistent with a model in which PP2A may
regulate mMOB1, striatin, and SG2NA to modulate changes in the
cytoskeleton or interactions between the cytoskeleton and membrane structures.
A Mammalian Homolog of Yeast MOB1 Is Both a Member and a
Putative Substrate of Striatin Family-Protein Phosphatase 2A
Complexes*
,¶
Department of Biochemistry and
Winship Cancer Center, Emory University School of Medicine, Atlanta,
Georgia 30322 and the § Harvard Microchemistry and
Proteomics Analysis Facility, Harvard University,
Cambridge, Massachusetts 02138
*
This work was supported by National Institutes of Health
Grant CA57327.The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.
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