| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 276, Issue 26, 24331-24340, June 29, 2001
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
From the Neisseria gonorrhoeae express
opacity-associated (Opa) protein adhesins that mediate binding to
various members of the carcinoembryonic antigen-related cellular
adhesion molecule (CEACAM; previously CD66) receptor family.
Although human umbilical vein endothelial cells express little CEACAM
receptor in vitro, we found neisserial infection to induce
expression of CEACAM1, CEACAM1-3L, and CECAM1-4L splice
variants. This mediates an increased
Opa52-dependent binding of gonococci by these
cells. The induced receptor expression did not require bacterial Opa
expression, but it was more rapid with adherent bacteria. Because the
time course of induction was similar to that seen for induced
proinflammatory cytokines, we tested whether CEACAM1 expression could
be controlled by a similar mechanism. Gonococcal infection activated a
nuclear factor-
Pathogenic Neisseria Trigger Expression of Their
Carcinoembryonic Antigen-related Cellular Adhesion Molecule 1 (CEACAM1;
Previously CD66a) Receptor on Primary Endothelial Cells by Activating
the Immediate Early Response Transcription Factor, Nuclear
Factor-
B*
§,§¶, and
**
Max-Planck-Institut für Biologie,
Abteilung Infektionsbiologie, Spemannstrasse 34, 72076 Tübingen,
the § Max-Planck-Institut für Infektionsbiologie,
Abteilung Molekulare Biologie, Schumannstrasse 21/22, Berlin (Mitte)
D-10117, Germany, and the Department of Medical Genetics and
Microbiology, University of Toronto, Toronto M5S 1A8, Canada.
B (NF-B) heterodimer consisting of p50 and p65,
and inhibitors that prevent the nuclear translocation of activated
NF-B complex inhibited CEACAM1 transcript expression. Each of these
effects could be mimicked by using culture filtrates or purified
lipopolysaccharide instead of intact bacteria. Together, our results
support a model whereby the outer membrane "blebs" that are
actively released by gonococci trigger a Toll-like receptor-4-dependent activation of NF-B, which
up-regulates the expression of CEACAM1 to allow
Opa52-mediated neisserial binding. The regulation of
CEACAM1 expression by NF-B also implies a broader role for this
receptor in the general inflammatory response to infection.
*
This study was supported in part by Deutsche
Forschungsgemeinschaft Grants Me705/5-1 and Na292/5-2 and by the Fonds
der Chemischen Industrie.The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  M. J. Callaghan, C. Buckee, N. D. McCarthy, A. B. Ibarz Pavon, K. A. Jolley, S. Faust, S. J. Gray, E. B. Kaczmarski, M. Levin, J. S. Kroll, et al. Opa Protein Repertoires of Disease-Causing and Carried Meningococci J. Clin. Microbiol., September 1, 2008; 46(9): 3033 - 3041. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. E. Bookwalter, J. A. Jurcisek, S. D. Gray-Owen, S. Fernandez, G. McGillivary, and L. O. Bakaletz A Carcinoembryonic Antigen-Related Cell Adhesion Molecule 1 Homologue Plays a Pivotal Role in Nontypeable Haemophilus influenzae Colonization of the Chinchilla Nasopharynx via the Outer Membrane Protein P5-Homologous Adhesin Infect. Immun., January 1, 2008; 76(1): 48 - 55. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Q. Yu, E. M. C. Chow, H. Wong, J. Gu, O. Mandelboim, S. D. Gray-Owen, and M. A. Ostrowski CEACAM1 (CD66a) Promotes Human Monocyte Survival via a Phosphatidylinositol 3-Kinase- and AKT-dependent Pathway J. Biol. Chem., December 22, 2006; 281(51): 39179 - 39193. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A.-M. Bamberger, J. Briese, J. Gotze, I. Erdmann, H. M. Schulte, C. Wagener, and P. Nollau Stimulation of CEACAM1 expression by 12-O-tetradecanoylphorbol-13-acetate (TPA) and calcium ionophore A23187 in endometrial carcinoma cells Carcinogenesis, March 1, 2006; 27(3): 483 - 490. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. L. Servin Pathogenesis of Afa/Dr Diffusely Adhering Escherichia coli Clin. Microbiol. Rev., April 1, 2005; 18(2): 264 - 292. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Betis, P. Brest, V. Hofman, J. Guignot, I. Kansau, B. Rossi, A. Servin, and P. Hofman Afa/Dr Diffusely Adhering Escherichia coli Infection in T84 Cell Monolayers Induces Increased Neutrophil Transepithelial Migration, Which in Turn Promotes Cytokine-Dependent Upregulation of Decay-Accelerating Factor (CD55), the Receptor for Afa/Dr Adhesins Infect. Immun., April 1, 2003; 71(4): 1774 - 1783. [Abstract] [Full Text]
|
|
|
|
|
|
K. Maisey, G. Nardocci, M. Imarai, H. Cardenas, M. Rios, H. B. Croxatto, J. E. Heckels, M. Christodoulides, and L. A. Velasquez Expression of Proinflammatory Cytokines and Receptors by Human Fallopian Tubes in Organ Culture following Challenge with Neisseria gonorrhoeae Infect. Immun., January 1, 2003; 71(1): 527 - 532. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Caamano and C. A. Hunter NF-{kappa}B Family of Transcription Factors: Central Regulators of Innate and Adaptive Immune Functions Clin. Microbiol. Rev., July 1, 2002; 15(3): 414 - 429. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. M. Tato and C. A. Hunter Host-Pathogen Interactions: Subversion and Utilization of the NF-{kappa}B Pathway during Infection Infect. Immun., July 1, 2002; 70(7): 3311 - 3317. [Full Text] [PDF]
|
|
|
|
 |
|
 B. B. Singer, I. Scheffrahn, R. Heymann, K. Sigmundsson, R. Kammerer, and B. Obrink Carcinoembryonic Antigen-Related Cell Adhesion Molecule 1 Expression and Signaling in Human, Mouse, and Rat Leukocytes: Evidence for Replacement of the Short Cytoplasmic Domain Isoform by Glycosylphosphatidylinositol-Linked Proteins in Human Leukocytes J. Immunol., May 15, 2002; 168(10): 5139 - 5146. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Muenzner, O. Billker, T. F. Meyer, and M. Naumann Nuclear Factor-kappa B Directs Carcinoembryonic Antigen-related Cellular Adhesion Molecule 1 Receptor Expression in Neisseria gonorrhoeae-infected Epithelial Cells J. Biol. Chem., February 22, 2002; 277(9): 7438 - 7446. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
