|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 276, Issue 27, 24531-24539, July 6, 2001
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
From the Transcription Laboratory, Imperial Cancer Research Fund
Laboratories, 44 Lincoln's Inn Fields, London
WC2A 3PX, United Kingdom
Activation of the transcription factor serum
response factor (SRF) is dependent on Rho-controlled changes in
actin dynamics. We used pathway-specific inhibitors to compare the
roles of actin dynamics, extracellular signal-regulated kinase (ERK)
signaling, and phosphatidylinositol 3-kinase in signaling either
to SRF itself or to four cellular SRF target genes. Serum,
lysophosphatidic acid, platelet-derived growth factor, and phorbol
12-myristate 13-acetate (PMA) each activated transcription of a
stably integrated SRF reporter gene dependent on functional RhoA
GTPase. Inhibition of mitogen-activated protein kinase-ERK kinase
(MEK) signalling reduced activation of the SRF reporter by all stimuli
by about 50%, except for PMA, which was effectively blocked.
Inhibition of phosphatidylinositol 3-kinase slightly reduced reporter
activation by serum and lysophosphatidic acid but substantially
inhibited activation by platelet-derived growth factor and PMA.
Reporter induction by all stimuli was absolutely dependent on actin
dynamics. Regulation of the SRF (srf) and vinculin
(vcl) genes was similar to that of the SRF reporter gene;
activation by all stimuli was Rho-dependent and required
actin dynamics but was largely independent of MEK activity. In
contrast, activation of fos and egr1 occurred independently of RhoA and actin polymerization but was almost completely dependent on MEK activation. These results show that at
least two classes of SRF target genes can be distinguished on the basis
of their relative sensitivity to RhoA-actin and MEK-ERK signaling pathways.
Differential Usage of Signal Transduction Pathways Defines Two
Types of Serum Response Factor Target Gene*
and
*
The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
Supported by a predoctoral award from the Overseas Research
Student Scheme of the United Kingdom Committee of Vice Chancellors and Principals.
§
To whom correspondence should be addressed: Transcription
Laboratory, Rm. 401, Imperial Cancer Research Fund Laboratories, 44 Lincoln's Inn Fields, London WC2A 3PX, UK; Fax:
44-20-7269-3093.
Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  M. A. Balamotis, M. A. Pennella, J. L. Stevens, B. Wasylyk, A. S. Belmont, and A. J. Berk Complexity in Transcription Control at the Activation Domain-Mediator Interface Sci. Signal., May 5, 2009; 2(69): ra20 - ra20. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Muehlich, R. Wang, S.-M. Lee, T. C. Lewis, C. Dai, and R. Prywes Serum-Induced Phosphorylation of the Serum Response Factor Coactivator MKL1 by the Extracellular Signal-Regulated Kinase 1/2 Pathway Inhibits Its Nuclear Localization Mol. Cell. Biol., October 15, 2008; 28(20): 6302 - 6313. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Gary-Bobo, A. Parlakian, B. Escoubet, C. A. Franco, S. Clement, P. Bruneval, D. Tuil, D. Daegelen, D. Paulin, Z. Li, et al. Mosaic inactivation of the serum response factor gene in the myocardium induces focal lesions and heart failure Eur J Heart Fail, July 1, 2008; 10(7): 635 - 645. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Hoffmann, J. Ashouri, S. Wolter, A. Doerrie, O. Dittrich-Breiholz, H. Schneider, E. F. Wagner, J. Troppmair, N. Mackman, and M. Kracht Transcriptional Regulation of EGR-1 by the Interleukin-1-JNK-MKK7-c-Jun Pathway J. Biol. Chem., May 2, 2008; 283(18): 12120 - 12128. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Busche, A. Descot, S. Julien, H. Genth, and G. Posern Epithelial cell-cell contacts regulate SRF-mediated transcription via Rac-actin-MAL signalling J. Cell Sci., April 1, 2008; 121(7): 1025 - 1035. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Hou, E. W. Arvisais, C. Jiang, D.-b. Chen, S. K. Roy, J. L. Pate, T. R. Hansen, B. R. Rueda, and J. S. Davis Prostaglandin F2{alpha} Stimulates the Expression and Secretion of Transforming Growth Factor B1 Via Induction of the Early Growth Response 1 Gene (EGR1) in the Bovine Corpus Luteum Mol. Endocrinol., February 1, 2008; 22(2): 403 - 414. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Y. Winter, T. Dayaram, and S. J. Marriott Activation of the Human T-Cell Leukemia Virus Type 1 Long Terminal Repeat by the Ternary Complex Factor Elk-1 J. Virol., December 1, 2007; 81(23): 13075 - 13081. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. D. Gopinath, S. Narumiya, and J. Dhawan The RhoA effector mDiaphanous regulates MyoD expression and cell cycle progression via SRF-dependent and SRF-independent pathways J. Cell Sci., September 1, 2007; 120(17): 3086 - 3098. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. W. Tullai, M. E. Schaffer, S. Mullenbrock, G. Sholder, S. Kasif, and G. M. Cooper Immediate-Early and Delayed Primary Response Genes Are Distinct in Function and Genomic Architecture J. Biol. Chem., August 17, 2007; 282(33): 23981 - 23995. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. S. Parmacek Myocardin-Related Transcription Factors: Critical Coactivators Regulating Cardiovascular Development and Adaptation Circ. Res., March 16, 2007; 100(5): 633 - 644. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. P. Staus, A. L. Blaker, J. M. Taylor, and C. P. Mack Diaphanous 1 and 2 Regulate Smooth Muscle Cell Differentiation by Activating the Myocardin-Related Transcription Factors Arterioscler. Thromb. Vasc. Biol., March 1, 2007; 27(3): 478 - 486. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. M. Miano, X. Long, and K. Fujiwara Serum response factor: master regulator of the actin cytoskeleton and contractile apparatus Am J Physiol Cell Physiol, January 1, 2007; 292(1): C70 - C81. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Sun, K. Boyd, W. Xu, J. Ma, C. W. Jackson, A. Fu, J. M. Shillingford, G. W. Robinson, L. Hennighausen, J. K. Hitzler, et al. Acute Myeloid Leukemia-Associated Mkl1 (Mrtf-a) Is a Key Regulator of Mammary Gland Function Mol. Cell. Biol., August 1, 2006; 26(15): 5809 - 5826. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A.-I. Zaromytidou, F. Miralles, and R. Treisman MAL and Ternary Complex Factor Use Different Mechanisms To Contact a Common Surface on the Serum Response Factor DNA-Binding Domain. Mol. Cell. Biol., June 1, 2006; 26(11): 4134 - 4148. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. H. Brown, D. P. Del Re, and M. A. Sussman The Rac and Rho Hall of Fame: A Decade of Hypertrophic Signaling Hits Circ. Res., March 31, 2006; 98(6): 730 - 742. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Iyer, D. Chang, J. Marx, L. Wei, E. N. Olson, M. S. Parmacek, A. Balasubramanyam, and R. J. Schwartz Serum response factor MADS box serine -162 phosphorylation switches proliferation and myogenic gene programs PNAS, March 21, 2006; 103(12): 4516 - 4521. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Zhao, R. G. DiScipio, A. G. Wimmer, and I. U. Schraufstatter Regulation of CXCR4-Mediated Nuclear Translocation of Extracellular Signal-Related Kinases 1 and 2 Mol. Pharmacol., January 1, 2006; 69(1): 66 - 75. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Kumbrink, M. Gerlinger, and J. P. Johnson Egr-1 Induces the Expression of Its Corepressor Nab2 by Activation of the Nab2 Promoter Thereby Establishing a Negative Feedback Loop J. Biol. Chem., December 30, 2005; 280(52): 42785 - 42793. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Rene, M. Taulan, F. Iral, J. Doudement, A. L'Honore, C. Gerbon, J. Demaille, M. Claustres, and M.-C. Romey Binding of serum response factor to cystic fibrosis transmembrane conductance regulator CArG-like elements, as a new potential CFTR transcriptional regulation pathway Nucleic Acids Res., September 16, 2005; 33(16): 5271 - 5290. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. N. McLaughlin, M. R. Mazzoni, J. H. Cleator, L. Earls, A. L. Perdigoto, J. D. Brooks, J. A. S. Muldowney III, D. E. Vaughan, and H. E. Hamm Thrombin Modulates the Expression of a Set of Genes Including Thrombospondin-1 in Human Microvascular Endothelial Cells J. Biol. Chem., June 10, 2005; 280(23): 22172 - 22180. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Alberti, S. M. Krause, O. Kretz, U. Philippar, T. Lemberger, E. Casanova, F. F. Wiebel, H. Schwarz, M. Frotscher, G. Schutz, et al. Neuronal migration in the murine rostral migratory stream requires serum response factor PNAS, April 26, 2005; 102(17): 6148 - 6153. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Kuwahara, T. Barrientos, G. C. T. Pipes, S. Li, and E. N. Olson Muscle-Specific Signaling Mechanism That Links Actin Dynamics to Serum Response Factor Mol. Cell. Biol., April 15, 2005; 25(8): 3173 - 3181. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. van Riggelen, G. Buchwalter, U. Soto, J. De-Castro Arce, H. z. Hausen, B. Wasylyk, and F. Rosl Loss of Net as Repressor Leads to Constitutive Increased c-fos Transcription in Cervical Cancer Cells J. Biol. Chem., February 4, 2005; 280(5): 3286 - 3294. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Kasza, A. O'Donnell, K. Gascoigne, L. A. H. Zeef, A. Hayes, and A. D. Sharrocks The ETS Domain Transcription Factor Elk-1 Regulates the Expression of Its Partner Protein, SRF J. Biol. Chem., January 14, 2005; 280(2): 1149 - 1155. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Zhe, Y. Yang, and L. Schuger Imbalanced Plasminogen System in Lymphangioleiomyomatosis: Potential Role of Serum Response Factor Am. J. Respir. Cell Mol. Biol., January 1, 2005; 32(1): 28 - 34. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Zhang, G. Azhar, Y. Zhong, and J. Y. Wei Identification of a Novel Serum Response Factor Cofactor in Cardiac Gene Regulation J. Biol. Chem., December 31, 2004; 279(53): 55626 - 55632. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. R. Vickers, A. Kasza, I. A. Kurnaz, A. Seifert, L. A. H. Zeef, A. O'Donnell, A. Hayes, and A. D. Sharrocks Ternary Complex Factor-Serum Response Factor Complex-Regulated Gene Activity Is Required for Cellular Proliferation and Inhibition of Apoptotic Cell Death Mol. Cell. Biol., December 1, 2004; 24(23): 10340 - 10351. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B.R. Wamhoff, M.H. Hoofnagle, A. Burns, S. Sinha, O.G. McDonald, and G.K. Owens A G/C Element Mediates Repression of the SM22{alpha} Promoter Within Phenotypically Modulated Smooth Muscle Cells in Experimental Atherosclerosis Circ. Res., November 12, 2004; 95(10): 981 - 988. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Lockman, J. S. Hinson, M. D. Medlin, D. Morris, J. M. Taylor, and C. P. Mack Sphingosine 1-Phosphate Stimulates Smooth Muscle Cell Differentiation and Proliferation by Activating Separate Serum Response Factor Co-factors J. Biol. Chem., October 8, 2004; 279(41): 42422 - 42430. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. W. Tullai, M. E. Schaffer, S. Mullenbrock, S. Kasif, and G. M. Cooper Identification of Transcription Factor Binding Sites Upstream of Human Genes Regulated by the Phosphatidylinositol 3-Kinase and MEK/ERK Signaling Pathways J. Biol. Chem., May 7, 2004; 279(19): 20167 - 20177. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Zhuang, G. T. Nguyen, Y. Chen, T. Gudi, M. Eigenthaler, T. Jarchau, U. Walter, G. R. Boss, and R. B. Pilz Vasodilator-stimulated Phosphoprotein Activation of Serum-response Element-dependent Transcription Occurs Downstream of RhoA and Is Inhibited by cGMP-dependent Protein Kinase Phosphorylation J. Biol. Chem., March 12, 2004; 279(11): 10397 - 10407. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. I. Reinhold, D. G. McEwen, and M. C. Naski Fibroblast Growth Factor Receptor 3 Gene: Regulation by Serum Response Factor Mol. Endocrinol., January 1, 2004; 18(1): 241 - 251. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Ott, D. Iwanciw, A. Graness, K. Giehl, and M. Goppelt-Struebe Modulation of the Expression of Connective Tissue Growth Factor by Alterations of the Cytoskeleton J. Biol. Chem., November 7, 2003; 278(45): 44305 - 44311. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. E. Ward, J. Evans, and C. S. Thummel Genetic Modifier Screens in Drosophila Demonstrate a Role for Rho1 Signaling in Ecdysone-Triggered Imaginal Disc Morphogenesis Genetics, November 1, 2003; 165(3): 1397 - 1415. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Cen, A. Selvaraj, R. C. Burgess, J. K. Hitzler, Z. Ma, S. W. Morris, and R. Prywes Megakaryoblastic Leukemia 1, a Potent Transcriptional Coactivator for Serum Response Factor (SRF), Is Required for Serum Induction of SRF Target Genes Mol. Cell. Biol., September 15, 2003; 23(18): 6597 - 6608. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Zeidan, I. Nordstrom, S. Albinsson, U. Malmqvist, K. Sward, and P. Hellstrand Stretch-induced contractile differentiation of vascular smooth muscle: sensitivity to actin polymerization inhibitors Am J Physiol Cell Physiol, June 1, 2003; 284(6): C1387 - C1396. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Percipalle, N. Fomproix, K. Kylberg, F. Miralles, B. Bjorkroth, B. Daneholt, and N. Visa An actin-ribonucleoprotein interaction is involved in transcription by RNA polymerase II PNAS, May 27, 2003; 100(11): 6475 - 6480. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Huang, M. Paliouras, I. Rambaldi, P. Lasko, and M. Featherstone Nonmuscle Myosin Promotes Cytoplasmic Localization of PBX Mol. Cell. Biol., May 15, 2003; 23(10): 3636 - 3645. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. L'honore, N. J. Lamb, M. Vandromme, P. Turowski, G. Carnac, and A. Fernandez MyoD Distal Regulatory Region Contains an SRF Binding CArG Element Required for MyoD Expression in Skeletal Myoblasts and during Muscle Regeneration Mol. Biol. Cell, May 1, 2003; 14(5): 2151 - 2162. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Posern, A. Sotiropoulos, and R. Treisman Mutant Actins Demonstrate a Role for Unpolymerized Actin in Control of Transcription by Serum Response Factor Mol. Biol. Cell, December 1, 2002; 13(12): 4167 - 4178. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Lin, D. Wang, and W. Sadee Serum Response Factor Activation by Muscarinic Receptors via RhoA. NOVEL PATHWAY SPECIFIC TO M1 SUBTYPE INVOLVING CALMODULIN, CALCINEURIN, AND Pyk2 J. Biol. Chem., October 18, 2002; 277(43): 40789 - 40798. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Murai and R. Treisman Interaction of Serum Response Factor (SRF) with the Elk-1 B Box Inhibits RhoA-Actin Signaling to SRF and Potentiates Transcriptional Activation by Elk-1 Mol. Cell. Biol., October 15, 2002; 22(20): 7083 - 7092. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Gudi, J. C. Chen, D. E. Casteel, T. M. Seasholtz, G. R. Boss, and R. B. Pilz cGMP-dependent Protein Kinase Inhibits Serum-response Element-dependent Transcription by Inhibiting Rho Activation and Functions J. Biol. Chem., September 27, 2002; 277(40): 37382 - 37393. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Psichari, A. Balmain, D. Plows, V. Zoumpourlis, and A. Pintzas High Activity of Serum Response Factor in the Mesenchymal Transition of Epithelial Tumor Cells Is Regulated by RhoA Signaling J. Biol. Chem., August 9, 2002; 277(33): 29490 - 29495. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. J. Inman, F. J. Nicolas, J. F. Callahan, J. D. Harling, L. M. Gaster, A. D. Reith, N. J. Laping, and C. S. Hill SB-431542 Is a Potent and Specific Inhibitor of Transforming Growth Factor-beta Superfamily Type I Activin Receptor-Like Kinase (ALK) Receptors ALK4, ALK5, and ALK7 Mol. Pharmacol., July 1, 2002; 62(1): 65 - 74. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. Geneste, J. W. Copeland, and R. Treisman LIM kinase and Diaphanous cooperate to regulate serum response factor and actin dynamics J. Cell Biol., May 28, 2002; 157(5): 831 - 838. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Charvet, P. Auberger, S. Tartare-Deckert, A. Bernard, and M. Deckert Vav1 Couples T Cell Receptor to Serum Response Factor-dependent Transcription via a MEK-dependent Pathway J. Biol. Chem., May 3, 2002; 277(18): 15376 - 15384. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. R. Wiggin, A. Soloaga, J. M. Foster, V. Murray-Tait, P. Cohen, and J. S. C. Arthur MSK1 and MSK2 Are Required for the Mitogen- and Stress-Induced Phosphorylation of CREB and ATF1 in Fibroblasts Mol. Cell. Biol., April 15, 2002; 22(8): 2871 - 2881. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. A. Tice, I. Soloviev, and P. Polakis Activation of the Wnt Pathway Interferes with Serum Response Element-driven Transcription of Immediate Early Genes J. Biol. Chem., February 15, 2002; 277(8): 6118 - 6123. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. L. Stevens, G. T. Cantin, G. Wang, A. Shevchenko, A. Shevchenko, and A. J. Berk Transcription Control by E1A and MAP Kinase Pathway via Sur2 Mediator Subunit Science, April 26, 2002; 296(5568): 755 - 758. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |