JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M101605200 on May 7, 2001

J. Biol. Chem., Vol. 276, Issue 27, 24549-24556, July 6, 2001
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
276/27/24549    most recent
M101605200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Shetty, S.
Right arrow Articles by Idell, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Shetty, S.
Right arrow Articles by Idell, S.

Urokinase Induces Expression of Its Own Receptor in Beas2B Lung Epithelial Cells*

Sreerama ShettyDagger and Steven Idell

From the Department of Medical Specialties, The University of Texas Health Center, Tyler, Texas 75708

Interaction between the urokinase-type plasminogen activator (uPA) and its receptor (uPAR) localizes cellular proteolysis and promotes cellular proliferation and migration. The interaction between uPA and uPAR at the surface of epithelial cells thereby contributes to the pathogenesis of lung inflammation and neoplasia. In this study, we sought to determine if uPA itself alters uPAR expression by lung epithelial cells. uPA enhanced uPAR expression as well as 125I-uPA binding in Beas2B lung epithelial cells in a time- and concentration-dependent manner. The uPA-mediated induction of uPAR is not accomplished through its receptor and requires enzymatic activity. The low molecular weight fragment of uPA, lacking the receptor binding domain, was as potent as intact two-chain uPA in inducing expression of uPAR at the cell surface. Plasmin, the end product of plasminogen activation, did not alter uPA-mediated uPAR expression. Induction of uPAR by uPA represents a novel pathway by which epithelial cells can regulate uPAR-dependent cellular responses that may contribute to stromal remodeling in lung injury or neoplasia.

* This work was supported by NHLBI Grant R01-HL-62453-01 from the National Institutes of Health (to S. S.), and Grant R01 45018-06 (to S. I.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Dagger To whom correspondence should be addressed: Dept. of Medical Specialties, The University of Texas Health Center at Tyler, Lab C-6, Biomedical Research Bldg., 11937 U.S. Highway 271, Tyler, TX 75708. Tel.: 903-877-7668; Fax: 903-877-5627; E-mail: sreerama.shetty@uthct.edu.

Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.


This article has been cited by other articles:


Home page
 Mol. Cell. Biol.Home page
S. Shetty, T. Velusamy, S. Idell, P. Shetty, A. P. Mazar, Y. P. Bhandary, and R. S. Shetty
Regulation of Urokinase Receptor Expression by p53: Novel Role in Stabilization of uPAR mRNA
Mol. Cell. Biol., August 15, 2007; 27(16): 5607 - 5618.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
S. Shetty, T. Velusamy, S. Idell, H. Tang, and P. K. Shetty
Regulation of urokinase receptor expression by protein tyrosine phosphatases
Am J Physiol Lung Cell Mol Physiol, February 1, 2007; 292(2): L414 - L421.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
S. Shetty, G. N. Rao, D. B. Cines, and K. Bdeir
Urokinase induces activation of STAT3 in lung epithelial cells
Am J Physiol Lung Cell Mol Physiol, October 1, 2006; 291(4): L772 - L780.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
S. Shetty, M. Ganachari, M.-C. Liu, A. Azghani, H. Muniyappa, and S. Idell
Regulation of urokinase receptor expression by phosphoglycerate kinase is independent of its catalytic activity
Am J Physiol Lung Cell Mol Physiol, October 1, 2005; 289(4): L591 - L598.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Shetty, M. R. Gyetko, and A. P. Mazar
Induction of p53 by Urokinase in Lung Epithelial Cells
J. Biol. Chem., July 29, 2005; 280(30): 28133 - 28141.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
S. Shetty, H. Muniyappa, P. K. S. Halady, and S. Idell
Regulation of Urokinase Receptor Expression by Phosphoglycerate Kinase
Am. J. Respir. Cell Mol. Biol., July 1, 2004; 31(1): 100 - 106.
[Abstract] [Full Text] [PDF]

Home page
 Molecular Cancer TherapeuticsHome page
A. Salvi, B. Arici, G. De Petro, and S. Barlati
Small interfering RNA urokinase silencing inhibits invasion and migration of human hepatocellular carcinoma cells
Mol. Cancer Ther., June 1, 2004; 3(6): 671 - 678.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
S. Shetty and S. Idell
Urokinase Receptor mRNA Stability Involves Tyrosine Phosphorylation in Lung Epithelial Cells
Am. J. Respir. Cell Mol. Biol., January 1, 2004; 30(1): 69 - 75.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Shetty, K. Bdeir, D. B. Cines, and S. Idell
Induction of Plasminogen Activator Inhibitor-1 by Urokinase in Lung Epithelial Cells
J. Biol. Chem., May 9, 2003; 278(20): 18124 - 18131.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
S. Shetty, U. R. Pendurthi, P. K. S. Halady, A. O. Azghani, and S. Idell
Urokinase induces its own expression in Beas2B lung epithelial cells
Am J Physiol Lung Cell Mol Physiol, August 1, 2002; 283(2): L319 - L328.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2001 by the American Society for Biochemistry and Molecular Biology.