|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 276, Issue 27, 24654-24660, July 6, 2001
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
From the The chloroplast signal recognition particle
(cpSRP) consists of an evolutionarily conserved 54-kDa subunit
(cpSRP54) and a dimer of a unique 43-kDa subunit (cpSRP43).
cpSRP binds light-harvesting chlorophyll proteins (LHCPs) to form a
cpSRP/LHCP transit complex, which targets LHCP to the thylakoid
membrane. Previous studies showed that transit complex formation is
mediated through the binding of the L18 domain of LHCP to
cpSRP43. cpSRP43 is characterized by a four-ankyrin repeat
domain at the N terminus and two chromodomains at the C terminus. In
the present study we used the yeast two-hybrid system and in
vitro binding assays to analyze the function of different domains
of cpSRP43 in protein complex formation. We report here that the first
ankyrin repeat binds to the 18-amino acid domain on LHCP that binds to
cpSRP43, whereas the third and fourth ankyrin repeats are
involved in the dimerization of cpSRP43. We show further that the
interaction of cpSRP43 with cpSRP54 is mediated via binding of the
methionine-rich domain of cpSRP54 to the C-terminally located
chromodomains of cpSRP43. Both chromodomains contain essential elements
for binding cpSRP54, indicating that the closely spaced chromodomains
together create a single binding site for cpSRP54. In addition, our
data demonstrate that the interaction of cpSRP54 with the chromodomains
of cpSRP43 is enhanced indirectly by the dimerization motif of cpSRP43.
Functional Analysis of the Protein-interacting Domains of
Chloroplast SRP43*
,
Institut für Biologie III der
Rheinisch-Westfälische Technische Hochschule Aachen,
Worringerweg 1, 52074 Aachen, Germany, the § Department
d'Ecophysiologie Vegetale et de Microbiologie, Cadarache, F-13108, St.
Paul lez Durance Cedex, France, and ¶ Paradigm Genetics, Research
Triangle Park, North Carolina 27709
*
This work was supported by Deutsche Forschungsgemeinschaft
Grant SCHU 1163/2.The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
To whom correspondence should be addressed. Tel.:
49-241-805871; Fax: 49-241-8888181; E-mail:
schuenemann@bio3.rwth-aachen.de.
Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  V. Sivaraja, T. K. S. Kumar, P. S. T. Leena, A.-n. Chang, C. Vidya, R. L. Goforth, D. Rajalingam, K. Arvind, J.-L. Ye, J. Chou, et al. Three-Dimensional Solution Structures of the Chromodomains of cpSRP43 J. Biol. Chem., December 16, 2005; 280(50): 41465 - 41471. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Funke, T. Knechten, J. Ollesch, and D. Schunemann A Unique Sequence Motif in the 54-kDa Subunit of the Chloroplast Signal Recognition Particle Mediates Binding to the 43-kDa Subunit J. Biol. Chem., March 11, 2005; 280(10): 8912 - 8917. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. L. Goforth, E. C. Peterson, J. Yuan, M. J. Moore, A. D. Kight, M. B. Lohse, J. Sakon, and R. L. Henry Regulation of the GTPase Cycle in Post-translational Signal Recognition Particle-based Protein Targeting Involves cpSRP43 J. Biol. Chem., October 8, 2004; 279(41): 43077 - 43084. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Erler, D. Hirnet, U. Wissenbach, V. Flockerzi, and B. A. Niemeyer Ca2+-selective Transient Receptor Potential V Channel Architecture and Function Require a Specific Ankyrin Repeat J. Biol. Chem., August 13, 2004; 279(33): 34456 - 34463. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Asakura, T. Hirohashi, S. Kikuchi, S. Belcher, E. Osborne, S. Yano, I. Terashima, A. Barkan, and M. Nakai Maize Mutants Lacking Chloroplast FtsY Exhibit Pleiotropic Defects in the Biogenesis of Thylakoid Membranes PLANT CELL, January 1, 2004; 16(1): 201 - 214. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. W. Murcha, R. Lister, A. Y. Y. Ho, and J. Whelan Identification, Expression, and Import of Components 17 and 23 of the Inner Mitochondrial Membrane Translocase from Arabidopsis Plant Physiology, April 1, 2003; 131(4): 1737 - 1747. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |