JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M102357200 on May 11, 2001

J. Biol. Chem., Vol. 276, Issue 27, 25043-25048, July 6, 2001
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
276/27/25043    most recent
M102357200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Swertfeger, D. K.
Right arrow Articles by Hui, D. Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Swertfeger, D. K.
Right arrow Articles by Hui, D. Y.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Apolipoprotein E Receptor Binding Versus Heparan Sulfate Proteoglycan Binding in Its Regulation of Smooth Muscle Cell Migration and Proliferation*

Debi K. SwertfegerDagger and David Y. Hui§

From the Department of Pathology and Laboratory Medicine, University of Cincinnati College of Medicine, Cincinnati, Ohio 45267-0529

This study showed that synthetic peptides containing either a single copy or tandem repeat of the receptor binding domain sequence of apolipoprotein (apo) E, or a peptide containing its C-terminal heparin binding domain, apoE-(211-243), were all effective inhibitors of platelet-derived growth factor (PDGF)-stimulated smooth muscle cell proliferation. In contrast, only the peptide containing a tandem repeating unit of the receptor binding domain sequence of apoE, apoE-(141-155)2, was capable of inhibiting PDGF-directed smooth muscle cell migration. Peptide containing only a single unit of this sequence, apoE-(141-155), or the apoE-(211-243) peptide were ineffective in inhibiting PDGF-directed smooth muscle cell migration. Additional experiments showed that reductively methylated apoE, which is incapable of receptor binding yet retains its heparin binding capability, was equally effective as apoE in inhibiting PDGF-stimulated smooth muscle cell proliferation. However, reductively methylated apoE was unable to inhibit smooth muscle cell migration toward PDGF. Additionally, the receptor binding domain-specific apoE antibody 1D7 also mitigated the anti-migratory properties of apoE on smooth muscle cells. Finally, pretreatment of cells with heparinase failed to abolish apoE inhibition of smooth muscle cell migration. Taken together, these data documented that apoE inhibition of PDGF-stimulated smooth muscle cell proliferation is mediated by its binding to heparan sulfate proteoglycans, while its inhibition of cell migration is mediated through apoE binding to cell surface receptors.

* This work was supported by National Institutes of Health Grant HL61332.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Dagger Received Postdoctoral Fellowship 9920615V from the Southern and Ohio Valley Consortium of the American Heart Association.

§ To whom correspondence should be addressed: Dept. of Pathology and Laboratory Medicine, University of Cincinnati College of Medicine, 231 Albert Sabin Way, Cincinnati, OH 45267-0529. Tel.: 513-558-9152; Fax: 513-558-2141; E-mail: Huidy@email.uc.edu.

Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
A. Kawamura, D. Baitsch, R. Telgmann, R. Feuerborn, G. Weissen-Plenz, C. Hagedorn, K. Saku, S.-M. Brand-Herrmann, A. von Eckardstein, G. Assmann, et al.
Apolipoprotein E Interrupts Interleukin-1{beta} Signaling in Vascular Smooth Muscle Cells
Arterioscler. Thromb. Vasc. Biol., July 1, 2007; 27(7): 1610 - 1617.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
Z. W. Q. Moore and D. Y. Hui
Apolipoprotein E inhibition of vascular hyperplasia and neointima formation requires inducible nitric oxide synthase
J. Lipid Res., October 1, 2005; 46(10): 2083 - 2090.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Futamura, P. Dhanasekaran, T. Handa, M. C. Phillips, S. Lund-Katz, and H. Saito
Two-step Mechanism of Binding of Apolipoprotein E to Heparin: IMPLICATIONS FOR THE KINETICS OF APOLIPOPROTEIN E-HEPARAN SULFATE PROTEOGLYCAN COMPLEX FORMATION ON CELL SURFACES
J. Biol. Chem., February 18, 2005; 280(7): 5414 - 5422.
[Abstract] [Full Text] [PDF]

Home page
 J BiochemHome page
T. Aoki, D. Sato, Y. Li, T. Takino, H. Miyamori, and H. Sato
Cleavage of Apolipoprotein E by Membrane-Type Matrix Metalloproteinase-1 Abrogates Suppression of Cell Proliferation
J. Biochem., January 1, 2005; 137(1): 95 - 99.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
F. M. Harris, I. Tesseur, W. J. Brecht, Q. Xu, K. Mullendorff, S. Chang, T. Wyss-Coray, R. W. Mahley, and Y. Huang
Astroglial Regulation of Apolipoprotein E Expression in Neuronal Cells: IMPLICATIONS FOR ALZHEIMER'S DISEASE
J. Biol. Chem., January 30, 2004; 279(5): 3862 - 3868.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y. Zhu and D. Y. Hui
Apolipoprotein E Binding to Low Density Lipoprotein Receptor-related Protein-1 Inhibits Cell Migration via Activation of cAMP-dependent Protein Kinase A
J. Biol. Chem., September 19, 2003; 278(38): 36257 - 36263.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
H. Saito, P. Dhanasekaran, D. Nguyen, F. Baldwin, K. H. Weisgraber, S. Wehrli, M. C. Phillips, and S. Lund-Katz
Characterization of the Heparin Binding Sites in Human Apolipoprotein E
J. Biol. Chem., April 18, 2003; 278(17): 14782 - 14787.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
J. S. Cohn, M. Tremblay, R. Batal, H. Jacques, L. Veilleux, C. Rodriguez, P. H. R. Barrett, D. Dubreuil, M. Roy, L. Bernier, et al.
Effect of atorvastatin on plasma apoE metabolism in patients with combined hyperlipidemia
J. Lipid Res., September 1, 2002; 43(9): 1464 - 1471.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. Rolny, D. Spillmann, U. Lindahl, and L. Claesson-Welsh
Heparin Amplifies Platelet-derived Growth Factor (PDGF)- BB-induced PDGF alpha -Receptor but Not PDGF beta -Receptor Tyrosine Phosphorylation in Heparan Sulfate-deficient Cells. EFFECTS ON SIGNAL TRANSDUCTION AND BIOLOGICAL RESPONSES
J. Biol. Chem., May 24, 2002; 277(22): 19315 - 19321.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
P. Boucher, P. Liu, M. Gotthardt, T. Hiesberger, R. G. W. Anderson, and J. Herz
Platelet-derived Growth Factor Mediates Tyrosine Phosphorylation of the Cytoplasmic Domain of the Low Density Lipoprotein Receptor-related Protein in Caveolae
J. Biol. Chem., May 3, 2002; 277(18): 15507 - 15513.
[Abstract] [Full Text] [PDF]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
B. Zhu, C. A. Reardon, G. S. Getz, and D. Y. Hui
Both Apolipoprotein E and Immune Deficiency Exacerbate Neointimal Hyperplasia After Vascular Injury in Mice
Arterioscler. Thromb. Vasc. Biol., March 1, 2002; 22(3): 450 - 455.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y.-Y. Ho, R. J. Deckelbaum, Y. Chen, T. Vogel, and D. A. Talmage
Apolipoprotein E Inhibits Serum-stimulated Cell Proliferation and Enhances Serum-independent Cell Proliferation
J. Biol. Chem., November 9, 2001; 276(46): 43455 - 43462.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
D. K. Swertfeger, G. Bu, and D. Y. Hui
Low Density Lipoprotein Receptor-related Protein Mediates Apolipoprotein E Inhibition of Smooth Muscle Cell Migration
J. Biol. Chem., February 1, 2002; 277(6): 4141 - 4146.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2001 by the American Society for Biochemistry and Molecular Biology.