|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 276, Issue 27, 25078-25087, July 6, 2001
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
From the Instituto de Ciências Biomédicas, Departamento
de Bioquímica Médica, Universidade Federal do Rio de
Janeiro, Cidade Universitária, RJ 21941-590, Brazil
Sarcoplasmic reticulum vesicles of rabbit
skeletal muscle accumulate Ca2+ at the expense of ATP
hydrolysis. The heat released during the hydrolysis of each ATP
molecule varies depending on whether or not a Ca2+ gradient
is formed across the vesicle membrane. After Ca2+
accumulation, a part of the Ca2+-ATPase activity is not
coupled with Ca2+ transport (Yu, X., and Inesi, G. (1995)
J. Biol. Chem. 270, 4361-4367). I now show that both
the heat produced during substrate hydrolysis and the uncoupled ATPase
activity vary depending on the ADP/ATP ratio in the medium. With a low
ratio, the Ca2+ transport is exothermic, and the formation
of the gradient increases the amount of heat produced during the
hydrolysis of each ATP molecule cleaved. With a high ADP/ATP ratio, the
Ca2+ transport is endothermic, and formation of a gradient
increased the amount of heat absorbed from the medium. Heat is absorbed from the medium when the Ca2+ efflux is coupled with the
synthesis of ATP (5.7 kcal/mol of ATP). When there is no ATP synthesis,
the Ca2+ efflux is exothermic (14-16 kcal/Ca2+
mol). It is concluded that in the presence of a low ADP concentration the uncoupled ATPase activity is the dominant route of heat production. With a high ADP/ATP ratio, the uncoupled ATPase activity is abolished, and the Ca2+ transport is endothermic. The possible
correlation of these findings with thermogenesis and anoxia is discussed.
Uncoupled ATPase Activity and Heat Production by the
Sarcoplasmic Reticulum Ca2+-ATPase
REGULATION BY ADP*
*
This work was supported by grants from PRONEX - Financiadora
de Estudos e Projetos (FINEP), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and by
Fundação de Amparo à Pesquisa do Estado do Rio de
Janeiro (FAPERJ).The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
To whom correspondence may be addressed. Tel.: 55-21-270-1635;
Fax: 55-21-270-8647; E-mail: demeis@bioqmed.ufjr.br.
Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  A. P. Arruda, L. A. Ketzer, M. Nigro, A. Galina, D. P. Carvalho, and L. de Meis Cold Tolerance in Hypothyroid Rabbits: Role of Skeletal Muscle Mitochondria and Sarcoplasmic Reticulum Ca2+ ATPase Isoform 1 Heat Production Endocrinology, December 1, 2008; 149(12): 6262 - 6271. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. A. Mahmmoud Capsaicin Stimulates Uncoupled ATP Hydrolysis by the Sarcoplasmic Reticulum Calcium Pump J. Biol. Chem., August 1, 2008; 283(31): 21418 - 21426. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Inesi, D. Lewis, C. Toyoshima, A. Hirata, and L. de Meis Conformational Fluctuations of the Ca2+-ATPase in the Native Membrane Environment: EFFECTS OF pH, TEMPERATURE, CATALYTIC SUBSTRATES, AND THAPSIGARGIN J. Biol. Chem., January 11, 2008; 283(2): 1189 - 1196. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Mall, R. Broadbridge, S. L. Harrison, M. G. Gore, A. G. Lee, and J. M. East The Presence of Sarcolipin Results in Increased Heat Production by Ca2+-ATPase J. Biol. Chem., December 1, 2006; 281(48): 36597 - 36602. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. de Meis, A. P. Arruda, R. M. da Costa, and M. Benchimol Identification of a Ca2+-ATPase in Brown Adipose Tissue Mitochondria: REGULATION OF THERMOGENESIS BY ATP AND Ca2+ J. Biol. Chem., June 16, 2006; 281(24): 16384 - 16390. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. E. Silva Thermogenic Mechanisms and Their Hormonal Regulation Physiol Rev, April 1, 2006; 86(2): 435 - 464. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Marrif, A. Schifman, Z. Stepanyan, M.-A. Gillis, A. Calderone, R. E. Weiss, J. Samarut, and J. E. Silva Temperature Homeostasis in Transgenic Mice Lacking Thyroid Hormone Receptor-{alpha} Gene Products Endocrinology, July 1, 2005; 146(7): 2872 - 2884. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. L. D. Riis, J. O. L. Jorgensen, N. Moller, J. Weeke, and T. Clausen Hyperthyroidism and cation pumps in human skeletal muscle Am J Physiol Endocrinol Metab, June 1, 2005; 288(6): E1265 - E1269. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. S. da-Silva, F. M. Bomfim, A. Galina, and L. de Meis Heat of PPi Hydrolysis Varies Depending on the Enzyme Used: YEAST AND CORN VACUOLAR PYROPHOSPHATASE J. Biol. Chem., October 29, 2004; 279(44): 45613 - 45617. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. de Meis Brown Adipose Tissue Ca2+-ATPase: UNCOUPLED ATP HYDROLYSIS AND THERMOGENIC ACTIVITY J. Biol. Chem., October 24, 2003; 278(43): 41856 - 41861. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Soler, M.-I. Fortea, A. Lax, and F. Fernandez-Belda Dissecting the Hydrolytic Activities of Sarcoplasmic Reticulum ATPase in the Presence of Acetyl Phosphate J. Biol. Chem., October 4, 2002; 277(41): 38127 - 38132. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Barata and L. de Meis Uncoupled ATP Hydrolysis and Thermogenic Activity of the Sarcoplasmic Reticulum Ca2+-ATPase. COUPLING EFFECTS OF DIMETHYL SULFOXIDE AND LOW TEMPERATURE J. Biol. Chem., May 3, 2002; 277(19): 16868 - 16872. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Sumbilla, D. Lewis, T. Hammerschmidt, and G. Inesi The Slippage of the Ca2+ Pump and Its Control by Anions and Curcumin in Skeletal and Cardiac Sarcoplasmic Reticulum J. Biol. Chem., April 12, 2002; 277(16): 13900 - 13906. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Tupling and H. Green Silver ions induce Ca2+ release from the SR in vitro by acting on the Ca2+ release channel and the Ca2+ pump J Appl Physiol, April 1, 2002; 92(4): 1603 - 1610. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. J. Logan-Smith, P. J. Lockyer, J. M. East, and A. G. Lee Curcumin, a Molecule That Inhibits the Ca2+-ATPase of Sarcoplasmic Reticulum but Increases the Rate of Accumulation of Ca2+ J. Biol. Chem., December 7, 2001; 276(50): 46905 - 46911. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Reis, M. Farage, A. C. L. de Souza, and L. de Meis Correlation between Uncoupled ATP Hydrolysis and Heat Production by the Sarcoplasmic Reticulum Ca2+-ATPase. COUPLING EFFECT OF FLUORIDE J. Biol. Chem., November 9, 2001; 276(46): 42793 - 42800. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |