Advertisement
JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M102227200 on May 7, 2001

J. Biol. Chem., Vol. 276, Issue 27, 25386-25391, July 6, 2001
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
276/27/25386    most recent
M102227200v1
Right arrow Submit a Letter to Editor
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowRequest Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Takagi, M.
Right arrow Articles by Yoneda, Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Takagi, M.
Right arrow Articles by Yoneda, Y.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

A Novel Nucleolar Protein, NIFK, Interacts with the Forkhead Associated Domain of Ki-67 Antigen in Mitosis*

Masatoshi TakagiDagger , Mari SueishiDagger , Takuya SaiwakiDagger , Ai KametakaDagger , and Yoshihiro YonedaDagger §

From the Dagger  Department of Cell Biology and Neuroscience, Graduate School of Medicine, Osaka University, 2-2 Yamada-oka, Suita, Osaka 565-0871 and the § Institute for Molecular and Cellular Biology, Osaka University, 1-3 Yamada-oka, Suita, Osaka 565-0871, Japan

In a previous study, we demonstrated that the forkhead associated (FHA) domain of pKi-67 interacts with the novel kinesin-like protein, Hklp2 (Sueishi, M., Takagi, M., and Yoneda, Y. (2000) J. Biol. Chem. 275, 28888-28892). In this study, we report on the identification of a putative RNA-binding protein of 293 residues as another binding partner of the FHA domain of pKi-67 (referred to as NIFK for nucleolar protein interacting with the FHA domain of pKi-67). Human NIFK (hNIFK) interacted with the FHA domain of pKi-67 (Ki-FHA) efficiently in vitro when hNIFK was derived from mitotically arrested cells. In addition, a moiety of hNIFK was co-localized with pKi-67 at the peripheral region of mitotic chromosomes. The hNIFK domain that interacts with Ki-FHA was mapped in the yeast two-hybrid system to a portion encompassed by residues 226-269. In a binding assay utilizing Xenopus egg extracts, it was found that the mitosis-specific environment and two threonine residues within this portion of hNIFK (Thr-234 and Thr-238) were crucial for the efficient interaction of hNIFK and Ki-FHA, suggesting that hNIFK interacts with Ki-FHA in a mitosis-specific and phosphorylation-dependent manner. These findings provide a new clue to our understanding of the cellular function of pKi-67.

* This work was supported by Grant-in-aid for Scientific Research on Priority Areas (B) 11237202, Grant-in-aid for Scientific Research (B) 12480215, Grant-in-aid for COE Research 12CE2007 from the Japanese Ministry of Education, Science, Sports and Culture, the Mitsubishi Foundation, and the Human Frontier Science Program.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

The nucleotide sequence(s) reported in this paper has been submitted to the GenBankTM/EMBL Data Bank with accession number(s) AB044971 and AB056870.

To whom correspondence should be addressed. Tel.: 81-6-6879-3210; Fax: 81-6-6879-3219; E-mail: yyoneda@anat3.med.osaka-u.ac.jp.

Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Sci SignalHome page
A. Mahajan, C. Yuan, H. Lee, E. S.-W. Chen, P.-Y. Wu, and M.-D. Tsai
Structure and Function of the Phosphothreonine-Specific FHA Domain
Sci. Signal., December 23, 2008; 1(51): re12 - re12.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
F. Fernandez-Madrid, N. Tang, H. Alansari, J. L. Granda, L. Tait, K. C. Amirikia, M. Moroianu, X. Wang, and R. L. Karvonen
Autoantibodies to Annexin XI-A and Other Autoantigens in the Diagnosis of Breast Cancer
Cancer Res., August 1, 2004; 64(15): 5089 - 5096.
[Abstract] [Full Text] [PDF]

Home page
 J. Histochem. Cytochem.Home page
T. Cheutin, M.-F. O'Donohue, A. Beorchia, C. Klein, H. Kaplan, and D. Ploton
Three-dimensional Organization of pKi-67: A Comparative Fluorescence and Electron Tomography Study Using Fluoronanogold
J. Histochem. Cytochem., November 1, 2003; 51(11): 1411 - 1423.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Boudrez, M. Beullens, E. Waelkens, W. Stalmans, and M. Bollen
Phosphorylation-dependent Interaction between the Splicing Factors SAP155 and NIPP1
J. Biol. Chem., August 23, 2002; 277(35): 31834 - 31841.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Fujiyama, M. Yanagida, T. Hayano, Y. Miura, T. Isobe, and N. Takahashi
Isolation and Proteomic Characterization of Human Parvulin-associating Preribosomal Ribonucleoprotein Complexes
J. Biol. Chem., June 21, 2002; 277(26): 23773 - 23780.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2001 by the American Society for Biochemistry and Molecular Biology.
Advertisement
spacer
Advertisement
Advertisement