|
Originally published In Press as doi:10.1074/jbc.M102402200 on May 22, 2001
J. Biol. Chem., Vol. 276, Issue 28, 26114-26121, July 13, 2001
Rapid Changes in Polyphosphate Content within Acidocalcisomes in
Response to Cell Growth, Differentiation, and Environmental Stress in
Trypanosoma cruzi*
Felix A.
Ruiz,
Claudia O.
Rodrigues, and
Roberto
Docampo
From the Laboratory of Molecular Parasitology, Department of
Pathobiology, University of Illinois at Urbana-Champaign,
Urbana, Illinois 61802
Inorganic polyphosphate (polyP) has been
identified and measured in different stages of Trypanosoma
cruzi. Millimolar levels (in terms of Pi
residues) in chains of less than 50 residues long, and micromolar
levels in chains of about 700-800 residues long, were found in
different stages of T. cruzi. Analysis of purified T. cruzi acidocalcisomes indicated that polyPs were preferentially located in these organelles. This was confirmed by visualization of
polyPs in the acidocalcisomes using
4',6-diamidino-2-phenylindole. A rapid increase (within 2-4 h)
in the levels of short and long chain polyPs was detected during
trypomastigote to amastigote differentiation and during the lag phase
of growth of epimastigotes (within 12-24 h). Levels rapidly decreased
after the epimastigotes resumed growth. Short and long chain polyP
levels rapidly decreased upon exposure of epimastigotes to hypo-osmotic
or alkaline stresses, whereas levels increased after hyperosmotic
stress. Ca2+ release from acidocalcisomes by a combination
of ionophores (ionomycin and nigericin) was associated with the
hydrolysis of short and long chain polyPs. In agreement with these
results, acidocalcisomes were shown to contain polyphosphate kinase and
exopolyphosphatase activities. Together, these results suggest a
critical role for these organelles in the adaptation of the parasite to
environmental changes.
*
This work was supported by National Institutes of Health
Grant AI-23259 (to R. D.).The costs of publication of this
article were defrayed in part by the
payment of page charges. The article must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
To whom correspondence should be addressed: Laboratory of
Molecular Parasitology, Dept. of Pathobiology, College of Veterinary Medicine, University of Illinois at Urbana-Champaign, 2001 S. Lincoln
Ave., Urbana, IL 61802. Tel.: 217-333-3845; Fax: 217-244-7421; E-mail:
rodoc@uiuc.edu.
Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.

CiteULike Complore Connotea Del.icio.us Digg Reddit Technorati What's this?
This article has been cited by other articles:

|
 |

|
 |
 
S. Besteiro, D. Tonn, L. Tetley, G. H. Coombs, and J. C. Mottram
The AP3 adaptor is involved in the transport of membrane proteins to acidocalcisomes of Leishmania
J. Cell Sci.,
March 1, 2008;
121(5):
561 - 570.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
J. Fang, F. A. Ruiz, M. Docampo, S. Luo, J. C. F. Rodrigues, L. S. Motta, P. Rohloff, and R. Docampo
Overexpression of a Zn2+-sensitive Soluble Exopolyphosphatase from Trypanosoma cruzi Depletes Polyphosphate and Affects Osmoregulation
J. Biol. Chem.,
November 2, 2007;
282(44):
32501 - 32510.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
B. Espiau, G. Lemercier, A. Ambit, F. Bringaud, G. Merlin, T. Baltz, and N. Bakalara
A Soluble Pyrophosphatase, a Key Enzyme for Polyphosphate Metabolism in Leishmania
J. Biol. Chem.,
January 20, 2006;
281(3):
1516 - 1523.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
K. Saito, R. Ohtomo, Y. Kuga-Uetake, T. Aono, and M. Saito
Direct Labeling of Polyphosphate at the Ultrastructural Level in Saccharomyces cerevisiae by Using the Affinity of the Polyphosphate Binding Domain of Escherichia coli Exopolyphosphatase
Appl. Envir. Microbiol.,
October 1, 2005;
71(10):
5692 - 5701.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
H. Zhang, M. R. Gomez-Garcia, M. R. W. Brown, and A. Kornberg
Inorganic polyphosphate in Dictyostelium discoideum: Influence on development, sporulation, and predation
PNAS,
February 22, 2005;
102(8):
2731 - 2735.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
P. Rohloff, A. Montalvetti, and R. Docampo
Acidocalcisomes and the Contractile Vacuole Complex Are Involved in Osmoregulation in Trypanosoma cruzi
J. Biol. Chem.,
December 10, 2004;
279(50):
52270 - 52281.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
M. Seufferheld, C. R. Lea, M. Vieira, E. Oldfield, and R. Docampo
The H+-pyrophosphatase of Rhodospirillum rubrum Is Predominantly Located in Polyphosphate-rich Acidocalcisomes
J. Biol. Chem.,
December 3, 2004;
279(49):
51193 - 51202.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
M. R. Gomez-Garcia and A. Kornberg
Formation of an actin-like filament concurrent with the enzymatic synthesis of inorganic polyphosphate
PNAS,
November 9, 2004;
101(45):
15876 - 15880.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
F. A. Ruiz, C. R. Lea, E. Oldfield, and R. Docampo
Human Platelet Dense Granules Contain Polyphosphate and Are Similar to Acidocalcisomes of Bacteria and Unicellular Eukaryotes
J. Biol. Chem.,
October 22, 2004;
279(43):
44250 - 44257.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
A. Montalvetti, P. Rohloff, and R. Docampo
A Functional Aquaporin Co-Localizes with the Vacuolar Proton Pyrophosphatase to Acidocalcisomes and the Contractile Vacuole Complex of Trypanosoma cruzi
J. Biol. Chem.,
September 10, 2004;
279(37):
38673 - 38682.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
J. A. Urbina, J. L. Concepcion, A. Caldera, G. Payares, C. Sanoja, T. Otomo, and H. Hiyoshi
In Vitro and In Vivo Activities of E5700 and ER-119884, Two Novel Orally Active Squalene Synthase Inhibitors, against Trypanosoma cruzi
Antimicrob. Agents Chemother.,
July 1, 2004;
48(7):
2379 - 2387.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
M. Seufferheld, M. C. F. Vieira, F. A. Ruiz, C. O. Rodrigues, S. N. J. Moreno, and R. Docampo
Identification of Organelles in Bacteria Similar to Acidocalcisomes of Unicellular Eukaryotes
J. Biol. Chem.,
August 8, 2003;
278(32):
29971 - 29978.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
K. J. Saliba, R. J. W. Allen, S. Zissis, P. G. Bray, S. A. Ward, and K. Kirk
Acidification of the Malaria Parasite's Digestive Vacuole by a H+-ATPase and a H+-pyrophosphatase
J. Biol. Chem.,
February 14, 2003;
278(8):
5605 - 5612.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
C. O. Rodrigues, F. A. Ruiz, M. Vieira, J. E. Hill, and R. Docampo
An Acidocalcisomal Exopolyphosphatase from Leishmania major with High Affinity for Short Chain Polyphosphate
J. Biol. Chem.,
December 20, 2002;
277(52):
50899 - 50906.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
C. O. Rodrigues, F. A. Ruiz, P. Rohloff, D. A. Scott, and S. N. J. Moreno
Characterization of Isolated Acidocalcisomes from Toxoplasma gondii Tachyzoites Reveals a Novel Pool of Hydrolyzable Polyphosphate
J. Biol. Chem.,
December 6, 2002;
277(50):
48650 - 48656.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
W. Chen, R. J. Palmer, and H. K. Kuramitsu
Role of Polyphosphate Kinase in Biofilm Formation by Porphyromonas gingivalis
Infect. Immun.,
August 1, 2002;
70(8):
4708 - 4715.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
M. J. McConville, K. A. Mullin, S. C. Ilgoutz, and R. D. Teasdale
Secretory Pathway of Trypanosomatid Parasites
Microbiol. Mol. Biol. Rev.,
March 1, 2002;
66(1):
122 - 154.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
N. Marchesini, F. A. Ruiz, M. Vieira, and R. Docampo
Acidocalcisomes Are Functionally Linked to the Contractile Vacuole of Dictyostelium discoideum
J. Biol. Chem.,
March 1, 2002;
277(10):
8146 - 8153.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
F. A. Ruiz, N. Marchesini, M. Seufferheld, Govindjee, and R. Docampo
The Polyphosphate Bodies of Chlamydomonas reinhardtii Possess a Proton-pumping Pyrophosphatase and Are Similar to Acidocalcisomes
J. Biol. Chem.,
November 30, 2001;
276(49):
46196 - 46203.
[Abstract]
[Full Text]
[PDF]
|
 |
|
Copyright © 2001 by the American Society for Biochemistry and Molecular Biology.
|
Advertisement
Advertisement
|