HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 276, Issue 28, 26211-26217, July 13, 2001

This Article Full Text Full Text (PDF) All Versions of this Article: 276/28/26211    most recent M100213200v1 Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Csar, X. F. Articles by Hamilton, J. A. Search for Related Content PubMed PubMed Citation Articles by Csar, X. F. Articles by Hamilton, J. A. Social Bookmarking                      What's this?

Proteomic Analysis of Macrophage Differentiation
p46/52^Shc TYROSINE PHOSPHORYLATION IS REQUIRED FOR CSF-1-MEDIATED MACROPHAGE DIFFERENTIATION^*

Xavier F. Csar^§¶, Nicholas J. Wilson^§, Kerrie-Ann McMahon, Denese C. Marks, Tina L. Beecroft^§, Alister C. Ward^**, Genevieve A. Whitty^§, Varuni Kanangasundarum^§, and John A. Hamilton^§

From the  Arthritis and Inflammation Research Centre, University of Melbourne, Department of Medicine, Royal Melbourne Hospital, Parkville, Victoria, Australia 3050, the ^§ Co-operative Research Centre for Chronic Inflammatory Diseases, Royal Melbourne Hospital, Parkville, Victoria, Australia 3050, the ^** Ludwig Institute for Cancer Research, Parkville, Victoria, Australia 3050, and the  Cell Cycle Proteolysis Laboratory, Trescowthick Research Laboratories, Peter MacCallum Cancer Institute, A'Beckett Street, Melbourne, Australia 3006

Macrophage colony stimulating factor (M-CSF or CSF-1) acts to regulate the development and function of cells of the macrophage lineage. Murine myeloid FDC-P1 cells transfected with the CSF-1 receptor (FD/WT) adopt a macrophage-like morphology when cultured in CSF-1. This process is abrogated in FDC-P1 cells transfected with the CSF-1 receptor with a tyrosine to phenyalanine substitution at position 807 (FD/807), suggesting that a molecular interaction critical to differentiation signaling is lost (Bourette, R. P., Myles, G. M., Carlberg, K., Chen, A. R., and Rohrschneider, L. R. (1995) Cell Growth Differ. 6, 631-645). A detailed examination of lysates of CSF-1-treated FD/807 cells by two-dimensional SDS-polyacrylamide gel electrophoresis (PAGE) revealed a number of proteins whose degree of tyrosine phosphorylation was modulated by the Y807F mutation. Included in this category were three phosphorylated proteins that co-migrated with p46/52^Shc. Immunoprecipitation, Western blotting, and in vitro binding studies suggest that they are indeed p46/52^Shc. A key regulator of differentiation in a number of cell systems, ERK was observed to exhibit an activity that correlated with the relative degree of differentiation induced by CSF-1 in the two cell types. Transfection of cells with a non-tyrosine-phosphorylatable form of p46/52^Shc prevented the normally observed CSF-1-mediated macrophage differentiation as determined by adoption of macrophage-like morphology and expression of the monocyte/macrophage lineage cell surface marker, Mac-1. These results are the first to suggest that p46/52^Shc may play a role in CSF-1-induced macrophage differentiation. Additionally, a number of proteins were identified by two-dimensional SDS-PAGE whose degree of tyrosine phosphorylation is also modulated by the Y807F substitution. This group of molecules may contain novel signaling molecules important in macrophage differentiation.

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				J. A. Hamilton, G. Whitty, P. Masendycz, N. J. Wilson, J. Jackson, D. De Nardo, and G. M. Scholz The Critical Role of the Colony-Stimulating Factor-1 Receptor in the Differentiation of Myeloblastic Leukemia Cells Mol. Cancer Res., March 1, 2008; 6(3): 458 - 467. [Abstract] [Full Text] [PDF]

				P. D. Simoncic, A. Bourdeau, A. Lee-Loy, L. R. Rohrschneider, M. L. Tremblay, E. R. Stanley, and C. J. McGlade T-cell protein tyrosine phosphatase (tcptp) is a negative regulator of colony-stimulating factor 1 signaling and macrophage differentiation. Mol. Cell. Biol., June 1, 2006; 26(11): 4149 - 4160. [Abstract] [Full Text] [PDF]

				J. Dauffy, G. Mouchiroud, and R. P. Bourette The interferon-inducible gene, Ifi204, is transcriptionally activated in response to M-CSF, and its expression favors macrophage differentiation in myeloid progenitor cells J. Leukoc. Biol., January 1, 2006; 79(1): 173 - 183. [Abstract] [Full Text] [PDF]

				C. Murdoch, A. Giannoudis, and C. E. Lewis Mechanisms regulating the recruitment of macrophages into hypoxic areas of tumors and other ischemic tissues Blood, October 15, 2004; 104(8): 2224 - 2234. [Abstract] [Full Text] [PDF]

				C. P. Baran, S. Tridandapani, C. D. Helgason, R. K. Humphries, G. Krystal, and C. B. Marsh The Inositol 5'-Phosphatase SHIP-1 and the Src Kinase Lyn Negatively Regulate Macrophage Colony-stimulating Factor-induced Akt Activity J. Biol. Chem., October 3, 2003; 278(40): 38628 - 38636. [Abstract] [Full Text] [PDF]

				J. R. Lee, Y. J. Ha, and H. J. Kim Cutting Edge: Induced Expression of a RhoA-Specific Guanine Nucleotide Exchange Factor, p190RhoGEF, Following CD40 Stimulation and WEHI 231 B Cell Activation J. Immunol., January 1, 2003; 170(1): 19 - 23. [Abstract] [Full Text] [PDF]

				N. Y. Bhatt, T. W. Kelley, V. V. Khramtsov, Y. Wang, G. K. Lam, T. L. Clanton, and C. B. Marsh Macrophage-Colony-Stimulating Factor-Induced Activation of Extracellular-Regulated Kinase Involves Phosphatidylinositol 3-Kinase and Reactive Oxygen Species in Human Monocytes J. Immunol., December 1, 2002; 169(11): 6427 - 6434. [Abstract] [Full Text] [PDF]

				R. Saban, N. P. Gerard, M. R. Saban, N.-B. Nguyen, D. J. DeBoer, and B. K. Wershil Mast cells mediate substance P-induced bladder inflammation through an NK1 receptor-independent mechanism Am J Physiol Renal Physiol, October 1, 2002; 283(4): F616 - F629. [Abstract] [Full Text] [PDF]

				D. Cappellen, N.-H. Luong-Nguyen, S. Bongiovanni, O. Grenet, C. Wanke, and M. Susa Transcriptional Program of Mouse Osteoclast Differentiation Governed by the Macrophage Colony-stimulating Factor and the Ligand for the Receptor Activator of NFkappa B J. Biol. Chem., June 7, 2002; 277(24): 21971 - 21982. [Abstract] [Full Text] [PDF]