|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 276, Issue 29, 26784-26791, July 20, 2001
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
From the The major surface protein of malaria sporozoites,
the circumsporozoite protein, binds to heparan sulfate proteoglycans on the surface of hepatocytes. It has been proposed that this binding event is responsible for the rapid and specific localization of sporozoites to the liver after their injection into the skin by an
infected anopheline mosquito. Previous in vitro studies
performed under static conditions have failed to demonstrate a
significant role for heparan sulfate proteoglycans during sporozoite
invasion of cells. We performed sporozoite attachment and invasion
assays under more dynamic conditions and found a dramatic decrease in sporozoite attachment to cells in the presence of heparin. In contrast
to its effect on attachment, heparin does not appear to have an effect
on sporozoite invasion of cells. When substituted heparins were used as
competitive inhibitors of sporozoite attachment, we found that
sulfation of the glycosaminoglycan chains at both the N-
and O-positions was important for sporozoite adhesion to cells. We conclude that the binding of the circumsporozoite protein to
hepatic heparan sulfate proteoglycans is likely to function during
sporozoite attachment in the liver and that this adhesion event depends
on the sulfated glycosaminoglycan chains of the proteoglycans.
The Binding of the Circumsporozoite Protein to Cell Surface
Heparan Sulfate Proteoglycans Is Required for Plasmodium
Sporozoite Attachment to Target Cells*
,,
Department of Medical and Molecular
Parasitology, New York University School of Medicine,
New York, New York 10010 and the ¶ Department of Cellular and
Molecular Medicine, University of California San Diego,
La Jolla, California 92093
*
This work was supported by a grant from the Irma T. Hirschl
Charitable Trust (to P. S.) and by National Institutes of Health Grants AI44470-02 (to P. S.) and GM33063 (to J. D. E.).The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
Current address: Dept. of Pediatrics, Boston Children's Hospital,
333 Longwood Ave., 2nd Floor, Health Services Research, Boston, MA 02115.
To whom correspondence should be addressed: Dept. of Medical
and Molecular Parasitology, New York University School of Medicine, 341 E. 25th St., New York, NY 10010. Tel.: 212-263-6818; Fax: 212-263-8116;
E-mail: photini.sinnis@med.nyu.edu.
Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  R. R. Dinglasan, A. Alaganan, A. K. Ghosh, A. Saito, T. H. van Kuppevelt, and M. Jacobs-Lorena Plasmodium falciparum ookinetes require mosquito midgut chondroitin sulfate proteoglycans for cell invasion PNAS, October 2, 2007; 104(40): 15882 - 15887. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Sinnis, A. Coppi, T. Toida, H. Toyoda, A. Kinoshita-Toyoda, J. Xie, M. M. Kemp, and R. J. Linhardt Mosquito Heparan Sulfate and Its Potential Role in Malaria Infection and Transmission J. Biol. Chem., August 31, 2007; 282(35): 25376 - 25384. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Coppi, M. Cabinian, D. Mirelman, and P. Sinnis Antimalarial activity of allicin, a biologically active compound from garlic cloves. Antimicrob. Agents Chemother., May 1, 2006; 50(5): 1731 - 1737. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Arca, F. Lombardo, J. G. Valenzuela, I. M. B. Francischetti, O. Marinotti, M. Coluzzi, and J. M. C. Ribeiro An updated catalogue of salivary gland transcripts in the adult female mosquito, Anopheles gambiae J. Exp. Biol., October 15, 2005; 208(20): 3971 - 3986. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. R. Bishop, B. E. Crawford, and J. D. Esko Cell Surface Heparan Sulfate Promotes Replication of Toxoplasma gondii Infect. Immun., September 1, 2005; 73(9): 5395 - 5401. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Coppi, C. Pinzon-Ortiz, C. Hutter, and P. Sinnis The Plasmodium circumsporozoite protein is proteolytically processed during cell invasion J. Exp. Med., January 3, 2005; 201(1): 27 - 33. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. B. Ancsin and R. Kisilevsky A Binding Site for Highly Sulfated Heparan Sulfate Is Identified in the N Terminus of the Circumsporozoite Protein: SIGNIFICANCE FOR MALARIAL SPOROZOITE ATTACHMENT TO HEPATOCYTES J. Biol. Chem., May 21, 2004; 279(21): 21824 - 21832. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Bork, N. Yokoyama, Y. Ikehara, S. Kumar, C. Sugimoto, and I. Igarashi Growth-Inhibitory Effect of Heparin on Babesia Parasites Antimicrob. Agents Chemother., January 1, 2004; 48(1): 236 - 241. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Tewari, R. Spaccapelo, F. Bistoni, A. A. Holder, and A. Crisanti Function of Region I and II Adhesive Motifs of Plasmodium falciparum Circumsporozoite Protein in Sporozoite Motility and Infectivity J. Biol. Chem., November 27, 2002; 277(49): 47613 - 47618. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Rathore, J. B. Sacci, P. de la Vega, and T. F. McCutchan Binding and Invasion of Liver Cells by Plasmodium falciparum Sporozoites. ESSENTIAL INVOLVEMENT OF THE AMINO TERMINUS OF CIRCUMSPOROZOITE PROTEIN J. Biol. Chem., February 22, 2002; 277(9): 7092 - 7098. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |