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J. Biol. Chem., Vol. 276, Issue 29, 26829-26837, July 20, 2001
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From the Department of Cell Biology and Anatomy, Medical University
of South Carolina, Charleston, South Carolina 29425-2204
Transcription regulatory domains of the
Prx1a and Prx1b homeoproteins were analyzed in transient transfection
assays using artificial promoters as well as an established downstream
target promoter (tenascin-c). Activation and repression domains were detected in their common amino end. In the carboxyl end of Prx1a an
activation domain and an inhibition/masking region (OAR domain) were
detected. The Prx1b isoform, generated by alternative splicing, does
not contain these carboxyl activation or inhibition domains. Instead,
the data demonstrate that the carboxyl tail of Prx1b contains a potent
repressor region. This difference in the carboxyl tail accounts for a
45-fold difference observed in transcription regulatory activity
between Prx1a and Prx1b. The data also support the likelihood that this
difference between Prx1a and Prx1b is higher in the presence of still
undetermined cofactors. DNA binding affinities of Prx1a, Prx1b, and a
series of truncation mutants were also examined. The carboxyl tail of
Prx1a, which inhibited transcription activation in the transfection
assays, also inhibited DNA binding. These differences in biochemical
function between Prx1a and Prx1b, as well as the recently described
activities of Prx2, provide a mechanism for the unequal compensation
between the Prx1 and Prx2 loci.
The Identification of Prx1 Transcription Regulatory Domains
Provides a Mechanism for Unequal Compensation by the
Prx1 and Prx2 Loci*
*
This work was supported by a National Institutes of Health
Grant HL-56596.The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
To whom correspondence should be addressed: Dept. of Cell Biology
and Anatomy, Medical University of South Carolina, 171 Ashley Ave.,
Charleston, SC 29425-2204. Tel.: 843-792-1774; Fax:
843-792-0664, E-mail: kernmj@musc.edu.
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