|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 276, Issue 29, 27480-27487, July 20, 2001
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
From the Membrane Biology Laboratory, Institute of Molecular and
Cell Biology, Singapore 117609, Singapore
The yeast SNARE Ykt6p has been implicated in
several trafficking steps, including vesicular transport from the
endoplasmic reticulum (ER) to the Golgi, intra-Golgi transport, and
homotypic vacuole fusion. The functional role of its mammalian
homologue (Ykt6) has not been established. Using antibodies specific
for mammalian Ykt6, it is revealed that it is found mainly in
Golgi-enriched membranes. Three SNAREs, syntaxin 5, GS28, and Bet1, are
specifically associated with Ykt6 as revealed by
co-immunoprecipitation, suggesting that these four SNAREs form a SNARE
complex. Double labeling of Ykt6 and the Golgi marker mannosidase II or
the ER-Golgi recycling marker KDEL receptor suggests that Ykt6 is
primarily associated with the Golgi apparatus. Unlike the KDEL
receptor, Ykt6 does not cycle back to the peripheral ER exit sites.
Antibodies against Ykt6 inhibit in vitro ER-Golgi transport
of vesicular stomatitis virus envelope glycoprotein (VSVG) only when
they are added before the EGTA-sensitive stage. ER-Golgi transport of
VSVG in vitro is also inhibited by recombinant Ykt6. In the
presence of antibodies against Ykt6, VSVG accumulates in peri-Golgi
vesicular structures and is prevented from entering the mannosidase II
compartment, suggesting that Ykt6 functions at a late stage in ER-Golgi
transport. Golgi apparatus marked by mannosidase II is fragmented into
vesicular structures in cells microinjected with Ykt6 antibodies. It is concluded that Ykt6 functions in a late step of ER-Golgi transport, and
this role may be important for the integrity of the Golgi complex.
Ykt6 Forms a SNARE Complex with Syntaxin 5, GS28,
and Bet1 and Participates in a Late Stage in Endoplasmic
Reticulum-Golgi Transport*
*
The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
To whom correspondence should be addressed: Inst. of Molecular and
Cell Biology, 30 Medical Dr., Singapore 117609, Singapore. Tel.:
65-778-6827; Fax: 65-779-1117; E-mail:
mcbhwj@imcb.nus.edu.sg.
Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  E. Wiederhold, T. Gandhi, H. P. Permentier, R. Breitling, B. Poolman, and D. J. Slotboom The Yeast Vacuolar Membrane Proteome Mol. Cell. Proteomics, February 1, 2009; 8(2): 380 - 392. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. V. Elias, R. Quiroga, N. Gottig, H. Nakanishi, T. E. Nash, A. Neiman, and H. D. Lujan Characterization of SNAREs Determines the Absence of a Typical Golgi Apparatus in the Ancient Eukaryote Giardia lamblia J. Biol. Chem., December 19, 2008; 283(51): 35996 - 36010. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Williams and J. E. Pessin Mapping of R-SNARE function at distinct intracellular GLUT4 trafficking steps in adipocytes J. Cell Biol., January 28, 2008; 180(2): 375 - 387. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. H. T. Tran, Q. Zeng, and W. Hong VAMP4 cycles from the cell surface to the trans-Golgi network via sorting and recycling endosomes J. Cell Sci., March 15, 2007; 120(6): 1028 - 1041. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. A. Siddiqi, S. Siddiqi, J. Mahan, K. Peggs, F. S. Gorelick, and C. M. Mansbach II The Identification of a Novel Endoplasmic Reticulum to Golgi SNARE Complex Used by the Prechylomicron Transport Vesicle J. Biol. Chem., July 28, 2006; 281(30): 20974 - 20982. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Appenzeller-Herzog and H.-P. Hauri The ER-Golgi intermediate compartment (ERGIC): in search of its identity and function J. Cell Sci., June 1, 2006; 119(11): 2173 - 2183. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. A. Siddiqi, J. Mahan, S. Siddiqi, F. S. Gorelick, and C. M. Mansbach II Vesicle-associated membrane protein 7 is expressed in intestinal ER. J. Cell Sci., March 1, 2006; 119(Pt 5): 943 - 950. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. E. P. Dietrich, T. J. LaGrassa, J. Rohde, M. Cristodero, C. T. A. Meiringer, and C. Ungermann ATP-independent Control of Vac8 Palmitoylation by a SNARE Subcomplex on Yeast Vacuoles J. Biol. Chem., April 15, 2005; 280(15): 15348 - 15355. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Loh, F. Peter, V. N. Subramaniam, and W. Hong Mammalian Bet3 functions as a cytosolic factor participating in transport from the ER to the Golgi apparatus J. Cell Sci., March 15, 2005; 118(6): 1209 - 1222. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Y. Lee, J.-S. Yang, W. Hong, R. T. Premont, and V. W. Hsu ARFGAP1 plays a central role in coupling COPI cargo sorting with vesicle formation J. Cell Biol., January 17, 2005; 168(2): 281 - 290. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Tai, L. Lu, T. L. Wang, B. L. Tang, B. Goud, L. Johannes, and W. Hong Participation of the Syntaxin 5/Ykt6/GS28/GS15 SNARE Complex in Transport from the Early/Recycling Endosome to the Trans-Golgi Network Mol. Biol. Cell, September 1, 2004; 15(9): 4011 - 4022. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Fukasawa, O. Varlamov, W. S. Eng, T. H. Sollner, and J. E. Rothman Localization and activity of the SNARE Ykt6 determined by its regulatory domain and palmitoylation PNAS, April 6, 2004; 101(14): 4815 - 4820. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Volchuk, M. Ravazzola, A. Perrelet, W. S. Eng, M. Di Liberto, O. Varlamov, M. Fukasawa, T. Engel, T. H. Sollner, J. E. Rothman, et al. Countercurrent Distribution of Two Distinct SNARE Complexes Mediating Transport within the Golgi Stack Mol. Biol. Cell, April 1, 2004; 15(4): 1506 - 1518. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Varlamov, A. Volchuk, V. Rahimian, C. A. Doege, F. Paumet, W. S. Eng, N. Arango, F. Parlati, M. Ravazzola, L. Orci, et al. i-SNAREs: inhibitory SNAREs that fine-tune the specificity of membrane fusion J. Cell Biol., January 5, 2004; 164(1): 79 - 88. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Hasegawa, S. Zinsser, Y. Rhee, E. O. Vik-Mo, S. Davanger, and J. C. Hay Mammalian Ykt6 Is a Neuronal SNARE Targeted to a Specialized Compartment by its Profilin-like Amino Terminal Domain Mol. Biol. Cell, February 1, 2003; 14(2): 698 - 720. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. B. Jang, Y.-G. Kim, Y.-S. Cho, P.-G. Suh, K.-H. Kim, and B.-H. Oh Crystal Structure of SEDL and Its Implications for a Genetic Disease Spondyloepiphyseal Dysplasia Tarda J. Biol. Chem., December 13, 2002; 277(51): 49863 - 49869. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Podgrabinska, P. Braun, P. Velasco, B. Kloos, M. S. Pepper, D. G. Jackson, and M. Skobe Molecular characterization of lymphatic endothelial cells PNAS, December 10, 2002; 99(25): 16069 - 16074. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Xu, S. Martin, D. E. James, and W. Hong GS15 Forms a SNARE Complex with Syntaxin 5, GS28, and Ykt6 and Is Implicated in Traffic in the Early Cisternae of the Golgi Apparatus Mol. Biol. Cell, October 1, 2002; 13(10): 3493 - 3507. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Liu and C. Barlowe Analysis of Sec22p in Endoplasmic Reticulum/Golgi Transport Reveals Cellular Redundancy in SNARE Protein Function Mol. Biol. Cell, September 1, 2002; 13(9): 3314 - 3324. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Loh and W. Hong Sec34 Is Implicated in Traffic from the Endoplasmic Reticulum to the Golgi and Exists in a Complex with GTC-90 and ldlBp J. Biol. Chem., June 7, 2002; 277(24): 21955 - 21961. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Shorter, M. B. Beard, J. Seemann, A. B. Dirac-Svejstrup, and G. Warren Sequential tethering of Golgins and catalysis of SNAREpin assembly by the vesicle-tethering protein p115 J. Cell Biol., April 1, 2002; 157(1): 45 - 62. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Shorter, M. B. Beard, J. Seemann, A. B. Dirac-Svejstrup, and G. Warren Sequential tethering of Golgins and catalysis of SNAREpin assembly by the vesicle-tethering protein p115 J. Cell Biol., April 1, 2002; 157(1): 45 - 62. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |