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Originally published In Press as doi:10.1074/jbc.M102778200 on April 27, 2001

J. Biol. Chem., Vol. 276, Issue 29, 27535-27540, July 20, 2001
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Crystal Structure of the Dimeric C-terminal Domain of TonB Reveals a Novel Fold*

Changsoo ChangDagger §, Alexandre Mooser§, Andreas Plückthun||, and Alexander WlodawerDagger

From the Dagger  Macromolecular Crystallography Laboratory, NCI, National Institutes of Health, Frederick, Maryland 21702 and the  Biochemisches Institut der Universität Zürich, Winterthurerstrasse 190, Zürich CH-8057, Switzerland

The TonB-dependent complex of Gram-negative bacteria couples the inner membrane proton motive force to the active transport of iron·siderophore and vitamin B12 across the outer membrane. The structural basis of that process has not been described so far in full detail. The crystal structure of the C-terminal domain of TonB from Escherichia coli has now been solved by multiwavelength anomalous diffraction and refined at 1.55-Å resolution, providing the first evidence that this region of TonB (residues 164-239) dimerizes. Moreover, the structure shows a novel architecture that has no structural homologs among any known proteins. The dimer of the C-terminal domain of TonB is cylinder-shaped with a length of 65 Å and a diameter of 25 Å. Each monomer contains three beta  strands and a single alpha  helix. The two monomers are intertwined with each other, and all six beta -strands of the dimer make a large antiparallel beta -sheet. We propose a plausible model of binding of TonB to FhuA and FepA, two TonB-dependent outer-membrane receptors.

* The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

§ Both authors contributed equally to this work.

|| To whom correspondence should be addressed: Biochemisches Institut der Universität Zürich, Winterthurerstrasse 190, CH-8057 Zürich, Switzerland. Tel.: 41-1-635-5570; Fax: 41-1-635-5712; E-mail: plueckthun@biocfebs.unizh.ch.

Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.
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