JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M101385200 on May 30, 2001

J. Biol. Chem., Vol. 276, Issue 30, 28179-28184, July 27, 2001
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
276/30/28179    most recent
M101385200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Col, E.
Right arrow Articles by Khochbin, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Col, E.
Right arrow Articles by Khochbin, S.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

The Histone Acetyltransferase, hGCN5, Interacts with and Acetylates the HIV Transactivator, Tat*

Edwige ColDagger , Cécile CaronDagger , Daphné Seigneurin-Berny§, Jocelyne GraciaDagger , Alain FavierDagger , and Saadi Khochbin§

From the Dagger  Laboratoire de Biologie du Stress Oxydant, Faculté de Pharmacie, Domaine de la Merci, 38700 La Tronche Cedex and the § Laboratoire de Biologie Moléculaire et Cellulaire de la Différenciation, INSERM U309, Institut Albert Bonniot, Faculté de Médecine, Domaine de la Merci, 38706 La Tronche Cedex, France

Factor acetyltransferase activity associated with several histone acetyltransferases plays a key role in the control of transcription. Here we report that hGCN5, a well known histone acetyltransferase, specifically interacts with and acetylates the human immunodeficiency virus type 1 (HIV-1) transactivator protein, Tat. The interaction between Tat and hGCN5 is direct and involves the acetyltransferase and the bromodomain regions of hGCN5, as well as a limited region of Tat encompassing the cysteine-rich domain of the protein. Tat lysines 50 and 51, target of acetylation by p300/CBP, were also found to be acetylated by hGCN5. The acetylation of these two lysines by p300/CBP has been recently shown to stimulate Tat transcriptional activity and accordingly, we have found that hGCN5 can considerably enhance Tat-dependent transcription of the HIV-1 long terminal repeat. These data highlight the importance of the acetylation of lysines 50 and 51 in the function of Tat, since different histone acetyltransferases involved in distinct signaling pathways, GCN5 and p300/CBP, converge to acetylate Tat on the same site.

* This work was supported by Sidaction Contract 991249/23026-01-00/AO10-1 (to S. K.) and Contract 000013/30041-03-00/AO10-1 (to C. C.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

To whom correspondence should be addressed. E-mail: khochbin@ujf-grenoble.fr.

Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Mol. Cell. Biol.Home page
A. Sabo, M. Lusic, A. Cereseto, and M. Giacca
Acetylation of Conserved Lysines in the Catalytic Core of Cyclin-Dependent Kinase 9 Inhibits Kinase Activity and Regulates Transcription
Mol. Cell. Biol., April 1, 2008; 28(7): 2201 - 2212.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Sharma, S. Awasthi, C. K. Harrod, E. F. Matlock, S. Khan, L. Xu, S. Chan, H. Yang, C. K. Thammavaram, R. A. Rasor, et al.
The Werner Syndrome Helicase Is a Cofactor for HIV-1 Long Terminal Repeat Transactivation and Retroviral Replication
J. Biol. Chem., April 20, 2007; 282(16): 12048 - 12057.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
N. Wiper-Bergeron, H. A. Salem, J. J. Tomlinson, D. Wu, and R. J. G. Hache
Glucocorticoid-stimulated preadipocyte differentiation is mediated through acetylation of C/EBPbeta by GCN5
PNAS, February 20, 2007; 104(8): 2703 - 2708.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. Mahmoudi, M. Parra, R. G. J. Vries, S. E. Kauder, C. P. Verrijzer, M. Ott, and E. Verdin
The SWI/SNF Chromatin-remodeling Complex Is a Cofactor for Tat Transactivation of the HIV Promoter
J. Biol. Chem., July 21, 2006; 281(29): 19960 - 19968.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
S. Ranjbar, R. Rajsbaum, and A. E. Goldfeld
Transactivator of Transcription from HIV Type 1 Subtype E Selectively Inhibits TNF Gene Expression via Interference with Chromatin Remodeling of the TNF Locus
J. Immunol., April 1, 2006; 176(7): 4182 - 4190.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
S. Amini, G. Mameli, L. Del Valle, A. Skowronska, K. Reiss, B. B. Gelman, M. K. White, K. Khalili, and B. E. Sawaya
p73 Interacts with Human Immunodeficiency Virus Type 1 Tat in Astrocytic Cells and Prevents Its Acetylation on Lysine 28
Mol. Cell. Biol., September 15, 2005; 25(18): 8126 - 8138.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
Y. Desfosses, M. Solis, Q. Sun, N. Grandvaux, C. Van Lint, A. Burny, A. Gatignol, M. A. Wainberg, R. Lin, and J. Hiscott
Regulation of Human Immunodeficiency Virus Type 1 Gene Expression by Clade-Specific Tat Proteins
J. Virol., July 15, 2005; 79(14): 9180 - 9191.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. Balasubramanyam, R. A. Varier, M. Altaf, V. Swaminathan, N. B. Siddappa, U. Ranga, and T. K. Kundu
Curcumin, a Novel p300/CREB-binding Protein-specific Inhibitor of Acetyltransferase, Represses the Acetylation of Histone/Nonhistone Proteins and Histone Acetyltransferase-dependent Chromatin Transcription
J. Biol. Chem., December 3, 2004; 279(49): 51163 - 51171.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y. Qiu, M. Guo, S. Huang, and R. Stein
Acetylation of the BETA2 Transcription Factor by p300-associated Factor Is Important in Insulin Gene Expression
J. Biol. Chem., March 12, 2004; 279(11): 9796 - 9802.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Biol.Home page
C. Jolly, A. Metz, J. Govin, M. Vigneron, B. M. Turner, S. Khochbin, and C. Vourc'h
Stress-induced transcription of satellite III repeats
J. Cell Biol., January 5, 2004; 164(1): 25 - 33.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
O. Rohr, C. Marban, D. Aunis, and E. Schaeffer
Regulation of HIV-1 gene transcription: from lymphocytes to microglial cells
J. Leukoc. Biol., November 1, 2003; 74(5): 736 - 749.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
A. Apolloni, C. W. Hooker, J. Mak, and D. Harrich
Human Immunodeficiency Virus Type 1 Protease Regulation of Tat Activity Is Essential for Efficient Reverse Transcription and Replication
J. Virol., September 15, 2003; 77(18): 9912 - 9921.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. Harrod, J. Nacsa, C. Van Lint, J. Hansen, T. Karpova, J. McNally, and G. Franchini
Human Immunodeficiency Virus Type-1 Tat/Co-activator Acetyltransferase Interactions Inhibit p53Lys-320 Acetylation and p53-responsive Transcription
J. Biol. Chem., March 28, 2003; 278(14): 12310 - 12318.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
V. Quivy, E. Adam, Y. Collette, D. Demonte, A. Chariot, C. Vanhulle, B. Berkhout, R. Castellano, Y. de Launoit, A. Burny, et al.
Synergistic Activation of Human Immunodeficiency Virus Type 1 Promoter Activity by NF-{kappa}B and Inhibitors of Deacetylases: Potential Perspectives for the Development of Therapeutic Strategies
J. Virol., October 2, 2002; 76(21): 11091 - 11103.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
E. Col, B. Gilquin, C. Caron, and S. Khochbin
Tat-controlled Protein Acetylation
J. Biol. Chem., September 27, 2002; 277(40): 37955 - 37960.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
V. Bres, R. Kiernan, S. Emiliani, and M. Benkirane
Tat Acetyl-acceptor Lysines Are Important for Human Immunodeficiency Virus Type-1 Replication
J. Biol. Chem., June 14, 2002; 277(25): 22215 - 22221.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
C. Merezak, M. Reichert, C. Van Lint, P. Kerkhofs, D. Portetelle, L. Willems, and R. Kettmann
Inhibition of Histone Deacetylases Induces Bovine Leukemia Virus Expression In Vitro and In Vivo
J. Virol., April 16, 2002; 76(10): 5034 - 5042.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2001 by the American Society for Biochemistry and Molecular Biology.