JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M102462200 on May 31, 2001

J. Biol. Chem., Vol. 276, Issue 31, 29178-29187, August 3, 2001
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
276/31/29178    most recent
M102462200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Goldshmidt, O.
Right arrow Articles by Vlodavsky, I.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Goldshmidt, O.
Right arrow Articles by Vlodavsky, I.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Expression Pattern and Secretion of Human and Chicken Heparanase Are Determined by Their Signal Peptide Sequence*

Orit GoldshmidtDagger , Eyal ZchariaDagger , Helena AingornDagger , Zehava Guatta-RanginiDagger , Ruth AtzmonDagger , Israel Michal§, Iris Pecker§, Eduardo Mitrani, and Israel VlodavskyDagger ||

From the Dagger  Department of Oncology, Hadassah-Hebrew University Hospital, Jerusalem 91120, § InSight Ltd., Rabin Science Park, Rehovot 76121, Israel, and the  Department of Cell and Animal Biology, The Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem, 91904 Israel

Cleavage of heparan sulfate (HS) proteoglycans affects the integrity and function of tissues and thereby fundamental phenomena, involving cell migration and response to changes in the extracellular microenvironment. The role of HS-degrading enzymes, commonly referred to as heparanases, in normal development has not been identified. The present study focuses on cloning, expression, and properties of a chicken heparanase and its distribution in the developing chicken embryo. We have identified a chicken EST, homologous to the recently cloned human heparanase, to clone and express a functional chicken heparanase, 60% homologous to the human enzyme. The full-length chicken heparanase cDNA encodes a 60-kDa proenzyme that is processed at the N terminus into a 45-kDa highly active enzyme. The most prominent difference between the chicken and human enzymes resides in the predicted signal peptide sequence, apparently accounting for the chicken heparanase being readily secreted and localized in close proximity to the cell surface. In contrast, the human enzyme is mostly intracellular, localized in perinuclear granules. Cells transfected with a chimeric construct composed of the chicken signal peptide preceding the human heparanase exhibited cell surface localization and secretion of heparanase, similar to cells transfected with the full-length chicken enzyme. We examined the distribution pattern of the heparanase enzyme in the developing chicken embryo. Both the chicken heparanase mRNA and protein were expressed, as early as 12 h post fertilization, in cells migrating from the epiblast and forming the hypoblast layer. Later on (72 h), the enzyme is preferentially expressed in cells of the developing vascular and nervous systems. Cloning and characterization of heparanase, the first and single functional vertebrate HS-degrading enzyme, may lead to identification of other glycosaminoglycan degrading enzymes, toward elucidation of their significance in normal and pathological processes.

* This work was supported by grants from the Cooperation program in Cancer Research of the Deutsches Krebforschungszentrum (DKFZ) and the Israeli's ministry of Science, the Science Foundation founded by the Israel Academy of Sciences and Humanities, the Israel Cancer Research Fund, and the Association for International Cancer Research, United Kingdom.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

The nucleotide sequence(s) reported in this paper has been submitted to the GenBankTM/EMBL Data Bank with accession number(s) AY037007.

|| To whom correspondence should be addressed: Dept. of Oncology, Hadassah Hospital, P. O. Box 12000, Jerusalem 91120, Israel. Tel.: 972-2-6776776; Fax: 972-2-6422794; E-mail: Vlodavsk@cc.huji.ac.il.

Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
G. E. Bertolesi, G. Michaiel, and S. McFarlane
Two Heparanase Splicing Variants with Distinct Properties Are Necessary in Early Xenopus Development
J. Biol. Chem., June 6, 2008; 283(23): 16004 - 16016.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
S. S D'Souza, A. T Fazleabas, P. Banerjee, J. R. A Sherwin, A. M Sharkey, M. C Farach-Carson, and D. D Carson
Decidual Heparanase Activity Is Increased During Pregnancy in the Baboon (Papio anubis) and in In Vitro Decidualization of Human Stromal Cells
Biol Reprod, February 1, 2008; 78(2): 316 - 323.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
I. Lerner, L. Baraz, E. Pikarsky, A. Meirovitz, E. Edovitsky, T. Peretz, I. Vlodavsky, and M. Elkin
Function of Heparanase in Prostate Tumorigenesis: Potential for Therapy
Clin. Cancer Res., February 1, 2008; 14(3): 668 - 676.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
S. Simizu, T. Suzuki, M. Muroi, N. S. Lai, S. Takagi, N. Dohmae, and H. Osada
Involvement of Disulfide Bond Formation in the Activation of Heparanase
Cancer Res., August 15, 2007; 67(16): 7841 - 7849.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
I. Shafat, I. Vlodavsky, and N. Ilan
Characterization of Mechanisms Involved in Secretion of Active Heparanase
J. Biol. Chem., August 18, 2006; 281(33): 23804 - 23811.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
S. Benhamron, H. Nechushtan, I. Verbovetski, A. Krispin, G. Abboud-Jarrous, E. Zcharia, E. Edovitsky, E. Nahari, T. Peretz, I. Vlodavsky, et al.
Translocation of Active Heparanase to Cell Surface Regulates Degradation of Extracellular Matrix Heparan Sulfate upon Transmigration of Mature Monocyte-Derived Dendritic Cells.
J. Immunol., June 1, 2006; 176(11): 6417 - 6424.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
E. Edovitsky, I. Lerner, E. Zcharia, T. Peretz, I. Vlodavsky, and M. Elkin
Role of endothelial heparanase in delayed-type hypersensitivity
Blood, May 1, 2006; 107(9): 3609 - 3616.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
N. J. Nasser, E. Nevo, I. Shafat, N. Ilan, I. Vlodavsky, and A. Avivi
Adaptive evolution of heparanase in hypoxia-tolerant Spalax: Gene cloning and identification of a unique splice variant
PNAS, October 18, 2005; 102(42): 15161 - 15166.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
R. Haimov-Kochman, D. Prus, E. Zcharia, D. S. Goldman-Wohl, S. Natanson-Yaron, C. Greenfield, E. Y. Anteby, R. Reich, J. Orly, A. Tsafriri, et al.
Spatiotemporal Expression of Heparanase During Human and Rodent Ovarian Folliculogenesis
Biol Reprod, July 1, 2005; 73(1): 20 - 28.
[Abstract] [Full Text] [PDF]

Home page
 JNCI J Natl Cancer InstHome page
E. Edovitsky, M. Elkin, E. Zcharia, T. Peretz, and I. Vlodavsky
Heparanase Gene Silencing, Tumor Invasiveness, Angiogenesis, and Metastasis
J Natl Cancer Inst, August 18, 2004; 96(16): 1219 - 1230.
[Abstract] [Full Text] [PDF]

Home page
 GlycobiologyHome page
K. Mani, F. Cheng, S. Sandgren, J. van den Born, B. Havsmark, K. Ding, and L.-A. Fransson
The heparan sulfate-specific epitope 10E4 is NO-sensitive and partly inaccessible in glypican-1
Glycobiology, July 1, 2004; 14(7): 599 - 607.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
A. Zetser, F. Levy-Adam, V. Kaplan, S. Gingis-Velitski, Y. Bashenko, S. Schubert, M. Y. Flugelman, I. Vlodavsky, and N. Ilan
Processing and activation of latent heparanase occurs in lysosomes
J. Cell Sci., May 1, 2004; 117(11): 2249 - 2258.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
N. Sasaki, N. Higashi, T. Taka, M. Nakajima, and T. Irimura
Cell Surface Localization of Heparanase on Macrophages Regulates Degradation of Extracellular Matrix Heparan Sulfate
J. Immunol., March 15, 2004; 172(6): 3830 - 3835.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
E. ZCHARIA, S. METZGER, T. CHAJEK-SHAUL, H. AINGORN, M. ELKIN, Y. FRIEDMANN, T. WEINSTEIN, J.-P. LI, U. LINDAHL, and I. VLODAVSKY
Transgenic expression of mammalian heparanase uncovers physiological functions of heparan sulfate in tissue morphogenesis, vascularization, and feeding behavior
FASEB J, February 1, 2004; 18(2): 252 - 263.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Simizu, K. Ishida, M. K. Wierzba, and H. Osada
Secretion of Heparanase Protein Is Regulated by Glycosylation in Human Tumor Cell Lines
J. Biol. Chem., January 23, 2004; 279(4): 2697 - 2703.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
V. Levidiotis, C. Freeman, C. Tikellis, M. E. Cooper, and D. A. Power
Heparanase Is Involved in the Pathogenesis of Proteinuria as a Result of Glomerulonephritis
J. Am. Soc. Nephrol., January 1, 2004; 15(1): 68 - 78.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
A. Zetser, Y. Bashenko, H.-Q. Miao, I. Vlodavsky, and N. Ilan
Heparanase Affects Adhesive and Tumorigenic Potential of Human Glioma Cells
Cancer Res., November 15, 2003; 63(22): 7733 - 7741.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. S. Huang, F.-M. Tang, Y.-H. Huang, I-H. Liu, S.-C. Hsu, S.-T. Chen, and J. S. Huang
Cloning, Expression, Characterization, and Role in Autocrine Cell Growth of Cell Surface Retention Sequence Binding Protein-1
J. Biol. Chem., October 31, 2003; 278(44): 43855 - 43869.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
O. GOLDSHMIDT, E. ZCHARIA, M. COHEN, H. AINGORN, I. COHEN, L. NADAV, B.-Z. KATZ, B. GEIGER, and I. VLODAVSKY
Heparanase mediates cell adhesion independent of its enzymatic activity
FASEB J, June 1, 2003; 17(9): 1015 - 1025.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
F. Cheng, K. Mani, J. van den Born, K. Ding, M. Belting, and L.-A. Fransson
Nitric Oxide-dependent Processing of Heparan Sulfate in Recycling S-Nitrosylated Glypican-1 Takes Place in Caveolin-1-containing Endosomes
J. Biol. Chem., November 8, 2002; 277(46): 44431 - 44439.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
O. Goldshmidt, E. Zcharia, R. Abramovitch, S. Metzger, H. Aingorn, Y. Friedmann, V. Schirrmacher, E. Mitrani, and I. Vlodavsky
Cell surface expression and secretion of heparanase markedly promote tumor angiogenesis and metastasis
PNAS, July 23, 2002; 99(15): 10031 - 10036.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2001 by the American Society for Biochemistry and Molecular Biology.