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Originally published In Press as doi:10.1074/jbc.M103936200 on June 6, 2001

J. Biol. Chem., Vol. 276, Issue 32, 29854-29863, August 10, 2001
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Response of an Integral Granule Membrane Protein to Changes in pH*

L. Chastine Bell-ParikhDagger , Betty A. Eipper§, and Richard E. Mains§

From the Department of Neuroscience, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205

A key feature of the regulated secretory pathway in neuroendocrine cells is lumenal pH, which decreases between trans-Golgi network and mature secretory granules. Because peptidylglycine alpha -amidating monooxygenase (PAM) is one of the few membrane-spanning proteins concentrated in secretory granules and is a known effector of regulated secretion, we examined its sensitivity to pH. Based on antibody binding experiments, the noncatalytic linker regions between the two enzymatic domains of PAM show pH-dependent conformational changes; these changes occur in the presence or absence of a transmembrane domain. Integral membrane PAM-1 solubilized from rat anterior pituitary or from transfected AtT-20 cells aggregates reversibly at pH 5.5 while retaining enzyme activity. Over 35% of the PAM-1 in anterior pituitary extracts aggregates at pH 5.5, whereas only about 5% aggregates at pH 7.5. PAM-1 recovered from secretory granules and endosomes is highly responsive to low pH-induced aggregation, whereas PAM-1 recovered from a light, intracellular recycling compartment is not. Mutagenesis studies indicate that a transmembrane domain is necessary but not sufficient for low pH-induced aggregation and reveal a short lumenal, juxtamembrane segment that also contributes to pH-dependent aggregation. Taken together, these results demonstrate that several properties of membrane PAM serve as indicators of granule pH in neuroendocrine cells.


* This work was supported by Grants DK32948, DK32949, and DK09942 from the National Institutes of Health.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Dagger Present address: Center for Experimental Therapeutics, University of Pennsylvania School of Medicine, Philadelphia, PA 19104.

§ Present address: Dept. of Neuroscience, University of Connecticut Health Center, 263 Farmington Ave., Farmington, CT 06030-3401.

To whom correspondence should be addressed: Dept. of Neuroscience, MC3401, University of Connecticut Health Center, 263 Farmington Ave., Farmington, CT 06030-3401. Tel.: 860-679-8894; Fax: 860-679-1060; E-mail: mains@uchc.edu.


Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.
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