|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 276, Issue 32, 30085-30091, August 10, 2001
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
From the Radiation Biology Branch, Division of Clinical Sciences,
NCI, National Institutes of Health, Bethesda, Maryland 20892
The quintessential nitrosating
species produced during NO autoxidation is
N2O3. Nitrosation of amine, thiol, and
hydroxyl residues can modulate critical cell functions. The biological mechanisms that control reactivity of nitrogen oxide species formed during autoxidation of nano- to micromolar levels of NO were examined using the synthetic donor NaEt2NN(O)NO (DEA/NO), human
tumor cells, and 4,5-diaminofluorescein (DAF). Both the disappearance
of NO and formation of nitrosated product from DAF in aerobic aqueous buffer followed second order processes; however, consumption of NO and
nitrosation within intact cells were exponential. An optimal ratio of
DEA/NO and 2-phenyl-4,4,5,5-tetramethylimidazole-1-oxyl 3-oxide (PTIO)
was used to form N2O3 through the intermediacy of NO2. This route was found to be most reflective of the
nitrosative mechanism within intact cells and was distinct from the
process that occurred during autoxidation of NO in aqueous media.
Manipulation of the endogenous scavengers ascorbate and glutathione
indicated that the location, affinity, and concentration of these
substances were key determinants in dictating nitrosative
susceptibility of molecular targets. Taken together, these findings
suggest that the functional effects of nitrosation may be organized to
occur within discrete domains or compartments. Nitrosative stress may develop when scavengers are depleted and this architecture becomes compromised. Although NO2 was not a component of aqueous NO
autoxidation, the results suggest that the intermediacy of this species
may be a significant factor in the advent of either nitrosation or oxidation chemistry in biological systems.
Distinction between Nitrosating Mechanisms within Human Cells and
Aqueous Solution*
,
*
The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
To whom correspondence should be addressed: Radiation Biology
Branch, NCI, National Institutes of Health, Bldg. 10, Rm. B3-B69, Bethesda, MD 20892. Tel.: 301-496-7511; Fax: 301-480-2238; E-mail: sp@nih.gov.
Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  R. Kovacs, A. Rabanus, J. Otahal, A. Patzak, J. Kardos, K. Albus, U. Heinemann, and O. Kann Endogenous Nitric Oxide Is a Key Promoting Factor for Initiation of Seizure-Like Events in Hippocampal and Entorhinal Cortex Slices J. Neurosci., July 1, 2009; 29(26): 8565 - 8577. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. A. Bosworth, J. C. Toledo Jr., J. W. Zmijewski, Q. Li, and J. R. Lancaster Jr. Dinitrosyliron complexes and the mechanism(s) of cellular protein nitrosothiol formation from nitric oxide PNAS, March 24, 2009; 106(12): 4671 - 4676. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. T. Gladwin and D. B. Kim-Shapiro The functional nitrite reductase activity of the heme-globins Blood, October 1, 2008; 112(7): 2636 - 2647. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Miersch, M. G. Espey, R. Chaube, A. Akarca, R. Tweten, S. Ananvoranich, and B. Mutus Plasma Membrane Cholesterol Content Affects Nitric Oxide Diffusion Dynamics and Signaling J. Biol. Chem., July 4, 2008; 283(27): 18513 - 18521. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Vitecek, V. Reinohl, and R. L. Jones Measuring NO Production by Plant Tissues and Suspension Cultured Cells Mol Plant, March 1, 2008; 1(2): 270 - 284. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Ckless, A. van der Vliet, and Y. Janssen-Heininger Arginase Modulates NF-{kappa}B Activity via a Nitric Oxide-Dependent Mechanism Am. J. Respir. Cell Mol. Biol., June 1, 2007; 36(6): 645 - 653. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 U. Rauen, T. Li, I. Ioannidis, and H. de Groot Nitric oxide increases toxicity of hydrogen peroxide against rat liver endothelial cells and hepatocytes by inhibition of hydrogen peroxide degradation Am J Physiol Cell Physiol, April 1, 2007; 292(4): C1440 - C1449. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. D. Thomas, L. A. Ridnour, M. G. Espey, S. Donzelli, S. Ambs, S. P. Hussain, C. C. Harris, W. DeGraff, D. D. Roberts, J. B. Mitchell, et al. Superoxide Fluxes Limit Nitric Oxide-induced Signaling J. Biol. Chem., September 8, 2006; 281(36): 25984 - 25993. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Planchet and W. M. Kaiser Nitric oxide (NO) detection by DAF fluorescence and chemiluminescence: a comparison using abiotic and biotic NO sources J. Exp. Bot., September 1, 2006; 57(12): 3043 - 3055. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Kilinc and A. Kilinc Mutagenic Actions of Nitrogen Oxides Indoor and Built Environment, December 1, 2005; 14(6): 503 - 512. [Abstract] [PDF]
|
|
|
|
|
|
V. M. Lakshmi, W. M. Nauseef, and T. V. Zenser Myeloperoxidase Potentiates Nitric Oxide-mediated Nitrosation J. Biol. Chem., January 21, 2005; 280(3): 1746 - 1753. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Zhang and N. Hogg Formation and stability of S-nitrosothiols in RAW 264.7 cells Am J Physiol Lung Cell Mol Physiol, September 1, 2004; 287(3): L467 - L474. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. J. Forman, J. M. Fukuto, and M. Torres Redox signaling: thiol chemistry defines which reactive oxygen and nitrogen species can act as second messengers Am J Physiol Cell Physiol, August 1, 2004; 287(2): C246 - C256. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. W. Foster and J. S. Stamler New Insights into Protein S-Nitrosylation: MITOCHONDRIA AS A MODEL SYSTEM J. Biol. Chem., June 11, 2004; 279(24): 25891 - 25897. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. S. Bryan, T. Rassaf, R. E. Maloney, C. M. Rodriguez, F. Saijo, J. R. Rodriguez, and M. Feelisch Cellular targets and mechanisms of nitros(yl)ation: An insight into their nature and kinetics in vivo PNAS, March 23, 2004; 101(12): 4308 - 4313. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. M. Tarpey, D. A. Wink, and M. B. Grisham Methods for detection of reactive metabolites of oxygen and nitrogen: in vitro and in vivo considerations Am J Physiol Regulatory Integrative Comp Physiol, March 1, 2004; 286(3): R431 - R444. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Goldstein, A. Russo, and A. Samuni Reactions of PTIO and Carboxy-PTIO with {middle dot}NO, {middle dot}NO2, and O3-. J. Biol. Chem., December 19, 2003; 278(51): 50949 - 50955. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. M. Kuebler, U. Uhlig, T. Goldmann, G. Schael, A. Kerem, K. Exner, C. Martin, E. Vollmer, and S. Uhlig Stretch Activates Nitric Oxide Production in Pulmonary Vascular Endothelial Cells In Situ Am. J. Respir. Crit. Care Med., December 1, 2003; 168(11): 1391 - 1398. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. M. Kuhn and T. J. Geddes Tetrahydrobiopterin Prevents Nitration of Tyrosine Hydroxylase by Peroxynitrite and Nitrogen Dioxide Mol. Pharmacol., October 1, 2003; 64(4): 946 - 953. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Park, T. J. Geddes, J. A. Javitch, and D. M. Kuhn Dopamine Prevents Nitration of Tyrosine Hydroxylase by Peroxynitrite and Nitrogen Dioxide: IS NITROTYROSINE FORMATION AN EARLY STEP IN DOPAMINE NEURONAL DAMAGE? J. Biol. Chem., August 1, 2003; 278(31): 28736 - 28742. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Jourd'heuil, F. L. Jourd'heuil, and M. Feelisch Oxidation and Nitrosation of Thiols at Low Micromolar Exposure to Nitric Oxide. EVIDENCE FOR A FREE RADICAL MECHANISM J. Biol. Chem., April 25, 2003; 278(18): 15720 - 15726. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. T. Jacobs and L. J. Ignarro Nuclear Factor-kappa B and Mitogen-activated Protein Kinases Mediate Nitric Oxide-enhanced Transcriptional Expression of Interferon-beta J. Biol. Chem., February 28, 2003; 278(10): 8018 - 8027. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Parthasarathi, H. Ichimura, S. Quadri, A. Issekutz, and J. Bhattacharya Mitochondrial Reactive Oxygen Species Regulate Spatial Profile of Proinflammatory Responses in Lung Venular Capillaries J. Immunol., December 15, 2002; 169(12): 7078 - 7086. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. S. Crane, R. Ollosson, K. P. Moore, A. G. Rossi, and I. L. Megson Novel Role for Low Molecular Weight Plasma Thiols in Nitric Oxide-mediated Control of Platelet Function J. Biol. Chem., November 27, 2002; 277(49): 46858 - 46863. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. G. Espey, D. D. Thomas, K. M. Miranda, and D. A. Wink Focusing of nitric oxide mediated nitrosation and oxidative nitrosylation as a consequence of reaction with superoxide PNAS, August 20, 2002; 99(17): 11127 - 11132. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Munzel, A. Mulsch, and A. Kleschyov Mechanisms Underlying Nitroglycerin-Induced Superoxide Production in Platelets: Some Insight, More Questions Circulation, July 9, 2002; 106(2): 170 - 172. [Full Text] [PDF]
|
|
|
|
|
|
M. G. Espey, S. Xavier, D. D. Thomas, K. M. Miranda, and D. A. Wink Direct real-time evaluation of nitration with green fluorescent protein in solution and within human cells reveals the impact of nitrogen dioxide vs. peroxynitrite mechanisms PNAS, March 19, 2002; 99(6): 3481 - 3486. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |