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J. Biol. Chem., Vol. 276, Issue 32, 30261-30269, August 10, 2001

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Cloning of a Mouse 1,3 N-Acetylglucosaminyltransferase GlcNAc(1,3)Gal(1,4)Glc-ceramide Synthase Gene Encoding the Key Regulator of Lacto-series Glycolipid Biosynthesis^*

Timothy R. Henion^§, Dapeng Zhou^§¶, David P. Wolfer, Firoze B. Jungalwala, and Thierry Hennet^¶**

From the  Eunice Kennedy Shriver Center, University of Massachusetts Medical School, Waltham, Massachusetts 02452 and the Institutes of ^¶ Physiology and  Anatomy, University of Zürich, Winterthurerstrasse 190, 8057 Zürich, Switzerland

The distinction between the different classes of glycolipids is conditioned by the action of specific core transferases. The entry point for lacto-series glycolipids is catalyzed by the 1,3 N-acetylglucosaminyltransferase GlcNAc(1,3)Gal(1,4)Glc-ceramide (Lc3) synthase enzyme. The Lc3 synthase activity has been shown to be regulated during development, especially during brain morphogenesis. Here, we report the molecular cloning of a mouse gene encoding an Lc3 synthase enzyme. The mouse cDNA included an open reading frame of 1131 base pairs encoding a protein of 376 amino acids. The Lc3 synthase protein shared several structural motifs previously identified in the members of the 1,3 glycosyltransferase superfamily. The Lc3 synthase enzyme efficiently utilized the lactosyl ceramide glycolipid acceptor. The identity of the reaction products of Lc3 synthase-transfected CHOP2/1 cells was confirmed by thin-layer chromatography immunostaining using antibodies TE-8 and 1B2 that recognize Lc3 and Gal(1,4)GlcNAc(1,3)Gal(1,4)Glc-ceramide (nLc4) structures, respectively. In addition to the initiating activity for lacto-chain synthesis, the Lc3 synthase could extend the terminal N-acetyllactosamine unit of nLc4 and also had a broad specificity for gangliosides GA1, GM1, and GD1b to generate neolacto-ganglio hybrid structures. The mouse Lc3 synthase gene was mainly expressed during embryonic development. In situ hybridization analysis revealed that that the Lc3 synthase was expressed in most tissues at embryonic day 11 with elevated expression in the developing central nervous system. Postnatally, the expression was restricted to splenic B-cells, the placenta, and cerebellar Purkinje cells where it colocalized with HNK-1 reactivity. These data support a key role for the Lc3 synthase in regulating neolacto-series glycolipid synthesis during embryonic development.

The nucleotide sequence(s) reported in this paper has been submitted to the GenBank^TM/EMBL Data Bank with accession number(s) AY029203 and AF368169.

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