|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 276, Issue 32, 30285-30292, August 10, 2001
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
From the Department of Medical Physiology and Cardiovascular
Research Institute and the The L-type calcium channel is the major calcium
influx pathway in vascular smooth muscle and is regulated by
integrin ligands, suggesting an important link between extracellular
matrix and vascular tone regulation in tissue injury and remodeling. We
examined the role of integrin-linked tyrosine kinases and focal
adhesion proteins in regulation of L-type calcium current in single
vascular myocytes. Soluble tyrosine kinase inhibitors blocked the
increase in current produced by
Regulation of the L-type Calcium Channel by
5
1 Integrin Requires Signaling between
Focal Adhesion Proteins*
, Department of Pathology and
Laboratory Medicine, Texas A&M University System Health Science Center,
College Station, Texas 77843-1114
5 integrin
antibody or fibronectin, whereas tyrosine phosphatase inhibition
enhanced the effect. Cell dialysis with an antibody to focal adhesion
kinase or with FRNK, the C-terminal noncatalytic domain of focal
adhesion kinase, produced moderate (24 or 18%, respectively)
inhibition of basal current but much greater inhibition (63 or 68%,
respectively) of integrin-enhanced current. A c-Src antibody and
peptide inhibitors of the Src homology-2 domain or a putative Src
tyrosine phosphorylation site on the channel produced similar
inhibition. Antibodies to the cytoskeletal proteins paxillin and
vinculin, but not -actinin, inhibited integrin-dependent current by 65-80%. Therefore, 5
1
integrin appears to regulate a tyrosine phosphorylation cascade
involving Src and various focal adhesion proteins that control the
function of the L-type calcium channel. This interaction may represent
a novel mechanism for control of calcium influx in vascular smooth
muscle and other cell types.
*
This study was supported by National Institutes of Health
Grants HL-46502 and HL-60180 (to M. J. D.), HL-55050 (to G. A. M.), and HL-59971 (to G. E. D.).The costs of publication of this
article were defrayed in part by the
payment of page charges. The article must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  Y. Yang, T. V. Murphy, S. R. Ella, T. H. Grayson, R. Haddock, Y. T. Hwang, A. P. Braun, G. Peichun, R. J. Korthuis, M. J. Davis, et al. Heterogeneity in function of small artery smooth muscle BKCa: involvement of the \#946;1-subunit J. Physiol., June 15, 2009; 587(12): 3025 - 3044. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Dai, D. D. Hall, and J. W. Hell Supramolecular Assemblies and Localized Regulation of Voltage-Gated Ion Channels Physiol Rev, April 1, 2009; 89(2): 411 - 452. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Shi, K. Jian, M. L. Ko, D. Trump, and G. Y.-P. Ko Retinoschisin, a New Binding Partner for L-type Voltage-gated Calcium Channels in the Retina J. Biol. Chem., February 6, 2009; 284(6): 3966 - 3975. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. A. Martinez-Lemus, M. A. Hill, and G. A. Meininger The Plastic Nature of the Vascular Wall: A Continuum of Remodeling Events Contributing to Control of Arteriolar Diameter and Structure Physiology, February 1, 2009; 24(1): 45 - 57. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. J. Gunst and W. Zhang Actin cytoskeletal dynamics in smooth muscle: a new paradigm for the regulation of smooth muscle contraction Am J Physiol Cell Physiol, September 1, 2008; 295(3): C576 - C587. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Sun, L. A. Martinez-Lemus, M. A. Hill, and G. A. Meininger Extracellular matrix-specific focal adhesions in vascular smooth muscle produce mechanically active adhesion sites Am J Physiol Cell Physiol, July 1, 2008; 295(1): C268 - C278. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. D. Tang and Y. Anfinogenova Physiologic Properties and Regulation of the Actin Cytoskeleton in Vascular Smooth Muscle Journal of Cardiovascular Pharmacology and Therapeutics, June 1, 2008; 13(2): 130 - 140. [Abstract] [PDF]
|
|
|
|
|
|
X. Wu, Y. Yang, P. Gui, Y. Sohma, G. A. Meininger, G. E. Davis, A. P. Braun, and M. J. Davis Potentiation of large conductance, Ca2+-activated K+ (BK) channels by {alpha}5{beta}1 integrin activation in arteriolar smooth muscle J. Physiol., March 15, 2008; 586(6): 1699 - 1713. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. Xue, L. Zhang, Y. Liu, S. J. Gunst, and R. S. Tepper Chronic inflation of ferret lungs with CPAP reduces airway smooth muscle contractility in vivo and in vitro J Appl Physiol, March 1, 2008; 104(3): 610 - 615. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Alessandri-Haber, O. A. Dina, E. K. Joseph, D. B. Reichling, and J. D. Levine Interaction of Transient Receptor Potential Vanilloid 4, Integrin, and Src Tyrosine Kinase in Mechanical Hyperalgesia J. Neurosci., January 30, 2008; 28(5): 1046 - 1057. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Kang, G. R. Ross, and H. I. Akbarali COOH-terminal association of human smooth muscle calcium channel Cav1.2b with Src kinase protein binding domains: effect of nitrotyrosylation Am J Physiol Cell Physiol, December 1, 2007; 293(6): C1983 - C1990. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Wang, L. M. McNamara, M. B. Schaffler, and S. Weinbaum A model for the role of integrins in flow induced mechanotransduction in osteocytes PNAS, October 2, 2007; 104(40): 15941 - 15946. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Wang, L. Weigand, J. Foxson, L. A. Shimoda, and J. T. Sylvester Ca2+ signaling in hypoxic pulmonary vasoconstriction: effects of myosin light chain and Rho kinase antagonists Am J Physiol Lung Cell Mol Physiol, September 1, 2007; 293(3): L674 - L685. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. A. Cobine, B. P. Callaghan, and K. D. Keef Role of L-type calcium channels and PKC in active tone development in rabbit coronary artery Am J Physiol Heart Circ Physiol, June 1, 2007; 292(6): H3079 - H3088. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. H.J. Heerkens, A. S. Izzard, and A. M. Heagerty Integrins, Vascular Remodeling, and Hypertension Hypertension, January 1, 2007; 49(1): 1 - 4. [Full Text] [PDF]
|
|
|
|
|
|
A. Umesh, M. A. Thompson, E. N. Chini, K.-P. Yip, and J. S. K. Sham Integrin Ligands Mobilize Ca2+ from Ryanodine Receptor-gated Stores and Lysosome-related Acidic Organelles in Pulmonary Arterial Smooth Muscle Cells J. Biol. Chem., November 10, 2006; 281(45): 34312 - 34323. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Callaghan, J. Zhong, and K. D. Keef Signaling pathway underlying stimulation of L-type Ca2+ channels in rabbit portal vein myocytes by recombinant Gbeta{gamma} subunits Am J Physiol Heart Circ Physiol, November 1, 2006; 291(5): H2541 - H2546. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Gui, X. Wu, S. Ling, S. C. Stotz, R. J. Winkfein, E. Wilson, G. E. Davis, A. P. Braun, G. W. Zamponi, and M. J. Davis Integrin Receptor Activation Triggers Converging Regulation of Cav1.2 Calcium Channels by c-Src and Protein Kinase A Pathways J. Biol. Chem., May 19, 2006; 281(20): 14015 - 14025. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. A. Martinez-Lemus, T. Crow, M. J. Davis, and G. A. Meininger {alpha}v{beta}3- and {alpha}5{beta}1-integrin blockade inhibits myogenic constriction of skeletal muscle resistance arterioles Am J Physiol Heart Circ Physiol, July 1, 2005; 289(1): H322 - H329. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S.J. Williams, B.G. White, and D.J. MacPhee Expression of {alpha}5 Integrin (Itga5) Is Elevated in the Rat Myometrium During Late Pregnancy and Labor: Implications for Development of a Mechanical Syncytium Biol Reprod, May 1, 2005; 72(5): 1114 - 1124. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
U. Rueckschloss and G. Isenberg Contraction augments L-type Ca2+ currents in adherent guinea-pig cardiomyocytes J. Physiol., October 15, 2004; 560(2): 403 - 411. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Jin, N. Morsy, J. Winston, P. J. Pasricha, K. Garrett, and H. I. Akbarali Modulation of TRPV1 by nonreceptor tyrosine kinase, c-Src kinase Am J Physiol Cell Physiol, August 1, 2004; 287(2): C558 - C563. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Callaghan, S.D. Koh, and K.D. Keef Muscarinic M2 Receptor Stimulation of Cav1.2b Requires Phosphatidylinositol 3-Kinase, Protein Kinase C, and c-Src Circ. Res., March 19, 2004; 94(5): 626 - 633. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Samii, M. H. Khan, D. A. MacLean, N. King, M. D. Herr, and L. I. Sinoway Muscle Interstitial Calcium During Head-Up Tilt in Humans Circulation, January 20, 2004; 109(2): 215 - 219. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. M. Browe and C. M. Baumgarten Stretch of {beta}1 Integrin Activates an Outwardly Rectifying Chloride Current via FAK and Src in Rabbit Ventricular Myocytes J. Gen. Physiol., November 24, 2003; 122(6): 689 - 702. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. E. Spurrell, T. V. Murphy, and M. A. Hill Intraluminal pressure stimulates MAPK phosphorylation in arterioles: temporal dissociation from myogenic contractile response Am J Physiol Heart Circ Physiol, October 1, 2003; 285(4): H1764 - H1773. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Lin, A. C. Arai, G. Lynch, and C. M. Gall Integrins Regulate NMDA Receptor-Mediated Synaptic Currents J Neurophysiol, May 1, 2003; 89(5): 2874 - 2878. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Campbell, M. Otis, M. Cote, N. Gallo-Payet, and M. D. Payet Connection between Integrins and Cell Activation in Rat Adrenal Glomerulosa Cells: A Role for Arg-Gly-Asp Peptide in the Activation of the p42/p44mapk Pathway and Intracellular Calcium Endocrinology, April 1, 2003; 144(4): 1486 - 1495. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. A. Kramar, J. A. Bernard, C. M. Gall, and G. Lynch Integrins Modulate Fast Excitatory Transmission at Hippocampal Synapses J. Biol. Chem., March 14, 2003; 278(12): 10722 - 10730. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. W. Carter and N. L. Kanagy Tyrosine kinases regulate intracellular calcium during alpha 2-adrenergic contraction in rat aorta Am J Physiol Heart Circ Physiol, October 1, 2002; 283(4): H1673 - H1680. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. E. Belevych, S. Warrier, and R. D. Harvey Genistein Inhibits Cardiac L-Type Ca2+ Channel Activity by a Tyrosine Kinase-Independent Mechanism Mol. Pharmacol., September 1, 2002; 62(3): 554 - 565. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. L. Lyford, P. R. Strege, A. Shepard, Y. Ou, L. Ermilov, S. M. Miller, S. J. Gibbons, J. L. Rae, J. H. Szurszewski, and G. Farrugia alpha 1C (CaV1.2) L-type calcium channel mediates mechanosensitive calcium regulation Am J Physiol Cell Physiol, September 1, 2002; 283(3): C1001 - C1008. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. D Tang, M.-F. Wu, A. M Opazo Saez, and S. J Gunst The focal adhesion protein paxillin regulates contraction in canine tracheal smooth muscle J. Physiol., July 15, 2002; 542(2): 501 - 513. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. O. Twal, A. Czirok, B. Hegedus, C. Knaak, M. R. Chintalapudi, H. Okagawa, Y. Sugi, and W. S. Argraves Fibulin-1 suppression of fibronectin-regulated cell adhesion and motility J. Cell Sci., March 14, 2002; 114(24): 4587 - 4598. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. C. Hool Can Integrins Integrate Vascular Myogenic Responses? Circ. Res., March 8, 2002; 90(4): 371 - 373. [Full Text] [PDF]
|
|
|
|
|
|
K. R. Waitkus-Edwards, L. A. Martinez-Lemus, X. Wu, J. P. Trzeciakowski, M. J. Davis, G. E. Davis, and G. A. Meininger {alpha}4{beta}1 Integrin Activation of L-Type Calcium Channels in Vascular Smooth Muscle Causes Arteriole Vasoconstriction Circ. Res., March 8, 2002; 90(4): 473 - 480. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. R. Waitkus-Edwards, L. A. Martinez-Lemus, X. Wu, J. P. Trzeciakowski, M. J. Davis, G. E. Davis, and G. A. Meininger {alpha}4{beta}1 Integrin Activation of L-Type Calcium Channels in Vascular Smooth Muscle Causes Arteriole Vasoconstriction Circ. Res., March 8, 2002; 90(4): 473 - 480. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |