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J. Biol. Chem., Vol. 276, Issue 33, 30995-31003, August 17, 2001
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From the mRNA degradation is a regulated process that
can play an important role in determining the level of expression of
specific genes. The rate at which a specific mRNA is degraded
depends largely on specific cis-acting sequences located
throughout the transcript. cis-Acting destabilizer
sequences that promote increased rates of decay have been identified in
several short-lived mRNAs. However, little is known about elements
that promote stability, known as stabilizer elements (STEs), and how
they function. The work presented here describes the characterization
of a STE in the PGK1 transcript. The PGK1 stabilizer element (P-STE)
has been delineated to a 64-nucleotide sequence from the coding region
that can stabilize a chimeric transcript containing the instability
elements from the 3'-untranslated region of the MFA2 transcript. The
P-STE is located within the PGK1 coding region and functions when
located in the translated portion of the transcript and at a minimum
distance from the 3'-untranslated region. These results further support
the link between translation and mRNA degradation. A conserved
sequence in the TEF1/2 transcript has been identified that also
functions as a STE, suggesting that this sequence element maybe a
general stability determinant found in other yeast mRNAs.
Characterization of a General Stabilizer Element That Blocks
Deadenylation-dependent mRNA Decay*
§¶,¶
,,, and Department of Molecular Genetics and
Microbiology, Robert Wood Johnson Medical School, University of
Medicine and Dentistry of New Jersey and the ** Cancer
Institute of New Jersey, Piscataway, New Jersey 08854
*
This work was supported in part by National Institutes of
Health Grants GM58276 (to S. W. P) and GM57483 (to T. G. K.), by an American Heart Association Established Investigator award
(to S. W. P.), and by an American Heart Association
grant-in-aid (to M. J. R.-E.).The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
Supported by a Howard Hughes undergraduate fellowship during
part of this research.
To whom correspondence may be addressed: Robert Wood Johnson
Medical School, University of Medicine and Dentistry of New Jersey, 675 Hoes Ln., Piscataway, NJ 08854. Tel.: 732-235-5450; Fax: 732-235-5223; E-mail: kinzytg@umdnj.edu.
§§
To whom correspondence may be addressed: Robert Wood Johnson
Medical School, University of Medicine and Dentistry of New Jersey, 675 Hoes Ln., Piscataway, NJ 08854. Tel.: 732-235-4790; Fax: 732-235-5223; E-mail: peltz@umdnj.edu.
Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.
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