JBC Invitrogen Ultrasensitive Cytokine Assays

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Originally published In Press as doi:10.1074/jbc.M102974200 on June 11, 2001

J. Biol. Chem., Vol. 276, Issue 33, 31429-31438, August 17, 2001
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DNA Synthesis by HIV-1 Reverse Transcriptase at the Central Termination Site
A KINETIC STUDY*

Marc LavigneDagger , Lucette Polomack§, and Henri Buc

From the Unité de Physicochimie des Macromolécules Biologiques, Institut Pasteur, URA1773 du CNRS, 75724 Paris Cedex 15, France

Human immunodeficiency virus, type 1 (HIV-1) reverse transcriptase (RT) terminates plus-strand DNA synthesis at the center of the HIV-1 genome, a process important for HIV-1 infectivity. The central termination sequence contains two termination sites (Ter1 and Ter2) located at the 3'-end of AnTm motifs, and the narrowing of the DNA minor groove generated by these motifs is responsible for termination. Kinetic data associated with the binding of RT and its ability to elongate in vitro various DNA duplexes and triplexes surrounding the Ter2 terminator were analyzed using a simple kinetic scheme. At Ter2, RT still displays a reasonable affinity for the corresponding DNA, but the binding of the next nucleotide and above all its incorporation rate are markedly hampered. Features affecting the width of the minor groove act directly at this last step. The constraint exerted against elongation by the AnTm tract persists at two positions downstream of the terminator.

* The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Dagger To whom correspondence should be addressed: Dept. of Molecular Biology, Wellman 10, Massachusetts General Hospital, Fruit St., Boston, MA 02114. E-mail: lavigne@molbio.mgh.harvard.edu.

§ Present address: Dept. of Molecular Biology, Institut Pasteur, 75724 Paris Cedex 15, France.

Present address: URA 1960 CNRS, Institut Pasteur, 75724 Paris Cedex 15, France.

Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.


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M. Lavigne, L. Polomack, and H. Buc
Structures of Complexes Formed by HIV-1 Reverse Transcriptase at a Termination Site of DNA Synthesis
J. Biol. Chem., August 10, 2001; 276(33): 31439 - 31448.
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