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J. Biol. Chem., Vol. 276, Issue 34, 31953-31958, August 24, 2001

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Recruitment of the Male-specific Lethal (MSL) Dosage Compensation Complex to an Autosomally Integrated roX Chromatin Entry Site Correlates with an Increased Expression of an Adjacent Reporter Gene in Male Drosophila^*

Rebecca A. Henry, Birke Tews, Xuelei Li, and Maxwell J. Scott

From the Institute of Molecular BioSciences, Massey University, Palmerston North 5320, New Zealand

Drosophila dosage compensate (equalize X-linked gene products) by doubling the transcription of most X-linked genes in males. The MSL (male-specific lethal) ribonucleoprotein complex consisting of at least five proteins and two non-coding RNAs (roX1 and roX2) is essential for this transcription response. Recently it has been shown that the X-linked roX1 and roX2 genes each contain at least one chromatin entry site for the MSL complex. In this study we show that insertion of either roX1 or roX2 DNA sequences, upstream of an insulated lacZ reporter gene controlled with the constitutive armadillo promoter (arm-lacZ), results in a significant elevation of expression of lacZ in males. However, full compensation, that is a precise doubling of lacZ expression in males relative to females, was only observed in some lines carrying autosomal insertions of either roX1-arm-lacZ or roX2-arm-lacZ transgenes. Furthermore, we found that a 419-base pair fragment of roX1 that contains an MSL binding site was sufficient to cause a modest elevation of expression of lacZ in males, but this response was significantly less than obtained with a full-length roX1 cDNA. This is the first direct demonstration that insertion of an MSL chromatin entry site on an autosome results in elevated expression in males of genes near the entry site.

This article has been cited by other articles:

				F. Li, A. H. Schiemann, and M. J. Scott Incorporation of the Noncoding roX RNAs Alters the Chromatin-Binding Specificity of the Drosophila MSL1/MSL2 Complex Mol. Cell. Biol., February 15, 2008; 28(4): 1252 - 1264. [Abstract] [Full Text] [PDF]

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				A. J. Greenberg, J. L. Yanowitz, and P. Schedl The Drosophila GAGA Factor Is Required for Dosage Compensation in Males and for the Formation of the Male-Specific-Lethal Complex Chromatin Entry Site at 12DE Genetics, January 1, 2004; 166(1): 279 - 289. [Abstract] [Full Text] [PDF]

				R. L. Kelley and M. I. Kuroda The Drosophila roX1 RNA Gene Can Overcome Silent Chromatin by Recruiting the Male-Specific Lethal Dosage Compensation Complex Genetics, June 1, 2003; 164(2): 565 - 574. [Abstract] [Full Text] [PDF]

				Y. Park, R. L. Kelley, H. Oh, M. I. Kuroda, and V. H. Meller Extent of Chromatin Spreading Determined by roX RNA Recruitment of MSL Proteins Science, November 22, 2002; 298(5598): 1620 - 1623. [Abstract] [Full Text] [PDF]