|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 276, Issue 34, 32071-32079, August 24, 2001
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
From the Various pathogenic bacteria such as
Shigella deliver effector proteins into mammalian cells via
the type III secretion system. The delivered Shigella
effectors have been shown to variously affect host functions required
for efficient bacterial internalization into the cells. In the present
study, we investigated the IpaH proteins for their ability to be
secreted via the type III secretion system and their fate in mammalian
cells. Upon incubation in a medium containing Congo red, the bacteria
secrete IpaH into the medium, but secretion of IpaH occurs later than
that of IpaBCD. Immunofluorescence microscopy indicated that
IpaH9.8 is secreted from intracellular bacteria and
transported into the nucleus. On microinjection of the protein,
intracellular IpaH9.8 is accumulated at one place around
the nucleus and transported into the nucleus. This movement seems to be
dependent on the microtubule network, since nuclear accumulation of
IpaH9.8 is inhibited in cells treated with
microtubule-destabilizing agents. In nuclear import assay, IpaH9.8 was efficiently transported into the nucleus, which
was completely blocked by treatment with wheat germ agglutinin. The nuclear transport of IpaH9.8 does not depend on host
cytosolic factors but is partially dependent on ATP/GTP, suggesting
that, like
Shigella Protein IpaH9.8 Is
Secreted from Bacteria within Mammalian Cells and Transported to the
Nucleus*
,,,
Division of Bacterial Infection, Department
of Microbiology and Immunology and the § Division of Cell
Biology and Biochemistry, Department of Basic Medical Sciences,
Institute of Medical Science, University of Tokyo, Minato-ku, Tokyo
108-8639 and the ¶ Department of Internal Medicine and
Therapeutics, Osaka University Graduate School of Medicine, Suita,
Osaka 565-0871, Japan
-catenin, IpaH9.8 secreted from intracellular
Shigella can be transported into the nucleus.
*
This work was supported by the Research for the Future
program of the Japan Society for the Promotion of Science and a
grant-in-aid for Scientific Research from the Japanese Ministry of
Education, Science, Sports, and Culture.The costs of publication of this article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
To whom correspondence should be addressed: Dept. of
Microbiology and Immunology, Institute of Medical Science, University of Tokyo, Minato-ku, Tokyo 108-8639, Japan. Tel.: 81-3-449-5252; Fax:
81-3-5449-5405; E-mail: sasakawa@ims.u-tokyo.ac.jp.
Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  G. N. Schroeder and H. Hilbi Molecular Pathogenesis of Shigella spp.: Controlling Host Cell Signaling, Invasion, and Death by Type III Secretion Clin. Microbiol. Rev., January 1, 2008; 21(1): 134 - 156. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. V. Zurawski, C. Mitsuhata, K. L. Mumy, B. A. McCormick, and A. T. Maurelli OspF and OspC1 Are Shigella flexneri Type III Secretion System Effectors That Are Required for Postinvasion Aspects of Virulence. Infect. Immun., October 1, 2006; 74(10): 5964 - 5976. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Miura, J. Terajima, H. Izumiya, J. Mitobe, T. Komano, and H. Watanabe OspE2 of Shigella sonnei Is Required for the Maintenance of Cell Architecture of Bacterium-Infected Cells. Infect. Immun., May 1, 2006; 74(5): 2587 - 2595. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Kuwae, T. Matsuzawa, N. Ishikawa, H. Abe, T. Nonaka, H. Fukuda, S. Imajoh-Ohmi, and A. Abe BopC Is a Novel Type III Effector Secreted by Bordetella bronchiseptica and Has a Critical Role in Type III-dependent Necrotic Cell Death J. Biol. Chem., March 10, 2006; 281(10): 6589 - 6600. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Yang, J. Yang, X. Zhang, L. Chen, Y. Jiang, Y. Yan, X. Tang, J. Wang, Z. Xiong, J. Dong, et al. Genome dynamics and diversity of Shigella species, the etiologic agents of bacillary dysentery Nucleic Acids Res., November 7, 2005; 33(19): 6445 - 6458. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Sabet, M. Lecuit, D. Cabanes, P. Cossart, and H. Bierne LPXTG Protein InlJ, a Newly Identified Internalin Involved in Listeria monocytogenes Virulence Infect. Immun., October 1, 2005; 73(10): 6912 - 6922. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Nagai, A. Abe, and C. Sasakawa Targeting of Enteropathogenic Escherichia coli EspF to Host Mitochondria Is Essential for Bacterial Pathogenesis: CRITICAL ROLE OF THE 16TH LEUCINE RESIDUE IN EspF J. Biol. Chem., January 28, 2005; 280(4): 2998 - 3011. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Lucchini, H. Liu, Q. Jin, J. C. D. Hinton, and J. Yu Transcriptional Adaptation of Shigella flexneri during Infection of Macrophages and Epithelial Cells: Insights into the Strategies of a Cytosolic Bacterial Pathogen Infect. Immun., January 1, 2005; 73(1): 88 - 102. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Dziva, P. M. van Diemen, M. P. Stevens, A. J. Smith, and T. S. Wallis Identification of Escherichia coli O157 : H7 genes influencing colonization of the bovine gastrointestinal tract using signature-tagged mutagenesis Microbiology, November 1, 2004; 150(11): 3631 - 3645. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Haraga and S. I. Miller A Salmonella enterica Serovar Typhimurium Translocated Leucine-Rich Repeat Effector Protein Inhibits NF-{kappa}B-Dependent Gene Expression Infect. Immun., July 1, 2003; 71(7): 4052 - 4058. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Wei, M. B. Goldberg, V. Burland, M. M. Venkatesan, W. Deng, G. Fournier, G. F. Mayhew, G. Plunkett III, D. J. Rose, A. Darling, et al. Complete Genome Sequence and Comparative Genomics of Shigella flexneri Serotype 2a Strain 2457T Infect. Immun., May 1, 2003; 71(5): 2775 - 2786. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Skrzypek, T. Myers-Morales, S. W. Whiteheart, and S. C. Straley Application of a Saccharomyces cerevisiae Model To Study Requirements for Trafficking of Yersinia pestis YopM in Eucaryotic Cells Infect. Immun., February 1, 2003; 71(2): 937 - 947. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Q. Jin, Z. Yuan, J. Xu, Y. Wang, Y. Shen, W. Lu, J. Wang, H. Liu, J. Yang, F. Yang, et al. Genome sequence of Shigella flexneri 2a: insights into pathogenicity through comparison with genomes of Escherichia coli K12 and O157 Nucleic Acids Res., October 15, 2002; 30(20): 4432 - 4441. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |