|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 276, Issue 34, 32101-32108, August 24, 2001
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
From the Department of Bacteriology, University of Wisconsin,
Madison, Wisconsin 53706
Homogeneous ferredoxin
(flavodoxin):NADP+ reductase and flavodoxin A
proteins served as electron donors for the reduction of co(III)rrinoids
to co(I)rrinoids in vitro. The resulting co(I)rrinoids served as substrates for the ATP:co(I)rrinoid
adenosyltransferase (CobA) enzyme of Salmonella enterica
serovar Typhimurium LT2 and were converted to their respective
adenosylated derivatives. The reaction products were isolated by
reverse phase high performance liquid chromatography, and their
identities were confirmed by UV-visible spectroscopy, mass
spectrometry, and in vivo biological activity assays.
Adenosylcobalamin generated by this system supported the activity of
1,2-propanediol dehydratase as effectively as authentic
adenosylcobalamin. This is the first report of a protein system that
can be coupled to the adenosyltransferase CobA enzyme for the
conversion of co(III)rrinoids to their adenosylated derivatives.
Use of this system is regulated by patent P00259US.
An in Vitro Reducing System for the Enzymic
Conversion of Cobalamin to Adenosylcobalamin*
and
*
This work was supported in part by National Institutes of
Health Grant GM40313 and by a DuPont Aid-To-Education grant (to J. C. E.-S.).The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
Recipient of a Minority Access to Research Careers (MARC)
predoctoral fellowship GM17528.
§
To whom correspondence should be addressed: Dept. of Bacteriology,
1550 Linden Dr., Madison, WI 53706-1567. Tel.: 608-262-7379; Fax: 608-262-9865; E-mail: jcescala@facstaff.wisc.edu.
Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  A. D. Lawrence, E. Deery, K. J. McLean, A. W. Munro, R. W. Pickersgill, S. E. J. Rigby, and M. J. Warren Identification, Characterization, and Structure/Function Analysis of a Corrin Reductase Involved in Adenosylcobalamin Biosynthesis J. Biol. Chem., April 18, 2008; 283(16): 10813 - 10821. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. C. Escalante-Semerena Conversion of Cobinamide into Adenosylcobamide in Bacteria and Archaea J. Bacteriol., July 1, 2007; 189(13): 4555 - 4560. [Full Text] [PDF]
|
|
|
|
|
|
M. St. Maurice, P. E. Mera, M. P. Taranto, F. Sesma, J. C. Escalante-Semerena, and I. Rayment Structural Characterization of the Active Site of the PduO-Type ATP:Co(I)rrinoid Adenosyltransferase from Lactobacillus reuteri J. Biol. Chem., January 26, 2007; 282(4): 2596 - 2605. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. R. Buan and J. C. Escalante-Semerena Purification and Initial Biochemical Characterization of ATP:Cob(I)alamin Adenosyltransferase (EutT) Enzyme of Salmonella enterica J. Biol. Chem., June 23, 2006; 281(25): 16971 - 16977. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. R. Buan, K. Rehfeld, and J. C. Escalante-Semerena Studies of the CobA-Type ATP:Co(I)rrinoid Adenosyltransferase Enzyme of Methanosarcina mazei Strain Go1. J. Bacteriol., May 1, 2006; 188(10): 3543 - 3550. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. R. Buan and J. C. Escalante-Semerena Computer-assisted Docking of Flavodoxin with the ATP:Co(I)rrinoid Adenosyltransferase (CobA) Enzyme Reveals Residues Critical for Protein-Protein Interactions but Not for Catalysis J. Biol. Chem., December 9, 2005; 280(49): 40948 - 40956. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. M. Sampson, C. L. V. Johnson, and T. A. Bobik Biochemical evidence that the pduS gene encodes a bifunctional cobalamin reductase Microbiology, April 1, 2005; 151(4): 1169 - 1177. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. A. Leal, H. Olteanu, R. Banerjee, and T. A. Bobik Human ATP:Cob(I)alamin Adenosyltransferase and Its Interaction with Methionine Synthase Reductase J. Biol. Chem., November 12, 2004; 279(46): 47536 - 47542. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. R. Buan, S.-J. Suh, and J. C. Escalante-Semerena The eutT Gene of Salmonella enterica Encodes an Oxygen-Labile, Metal-Containing ATP:Corrinoid Adenosyltransferase Enzyme J. Bacteriol., September 1, 2004; 186(17): 5708 - 5714. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Saridakis, A. Yakunin, X. Xu, P. Anandakumar, M. Pennycooke, J. Gu, F. Cheung, J. M. Lew, R. Sanishvili, A. Joachimiak, et al. The Structural Basis for Methylmalonic Aciduria: THE CRYSTAL STRUCTURE OF ARCHAEAL ATP:COBALAMIN ADENOSYLTRANSFERASE J. Biol. Chem., May 28, 2004; 279(22): 23646 - 23653. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. D. Woodson and J. C. Escalante-Semerena CbiZ, an amidohydrolase enzyme required for salvaging the coenzyme B12 precursor cobinamide in archaea PNAS, March 9, 2004; 101(10): 3591 - 3596. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. A. Rodionov, A. G. Vitreschak, A. A. Mironov, and M. S. Gelfand Comparative Genomics of the Vitamin B12 Metabolism and Regulation in Prokaryotes J. Biol. Chem., October 17, 2003; 278(42): 41148 - 41159. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. D. Woodson, R. F. Peck, M. P. Krebs, and J. C. Escalante-Semerena The cobY Gene of the Archaeon Halobacterium sp. Strain NRC-1 Is Required for De Novo Cobamide Synthesis J. Bacteriol., January 1, 2003; 185(1): 311 - 316. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. M. Dobson, T. Wai, D. Leclerc, H. Kadir, M. Narang, J. P. Lerner-Ellis, T. J. Hudson, D. S. Rosenblatt, and R. A. Gravel Identification of the gene responsible for the cblB complementation group of vitamin B12-dependent methylmalonic aciduria Hum. Mol. Genet., December 15, 2002; 11(26): 3361 - 3369. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. V. Fonseca, N. R. Buan, A. R. Horswill, I. Rayment, and J. C. Escalante-Semerena The ATP:Co(I)rrinoid Adenosyltransferase (CobA) Enzyme of Salmonella enterica Requires the 2'-OH Group of ATP for Function and Yields Inorganic Triphosphate as Its Reaction Byproduct J. Biol. Chem., August 30, 2002; 277(36): 33127 - 33131. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |