JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M102002200 on June 6, 2001

J. Biol. Chem., Vol. 276, Issue 34, 32122-32128, August 24, 2001
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
276/34/32122    most recent
M102002200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Boudreau, F.
Right arrow Articles by Traber, P. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Boudreau, F.
Right arrow Articles by Traber, P. G.

Sucrase-isomaltase Gene Transcription Requires the Hepatocyte Nuclear Factor-1 (HNF-1) Regulatory Element and Is Regulated by the Ratio of HNF-1alpha to HNF-1beta *

François BoudreauDagger , Yi Zhu, and Peter G. Traber§

From the Division of Gastroenterology, Department of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104

The mouse sucrase-isomaltase (SI) gene is an enterocyte-specific gene expressed in a complex developmental pattern. We previously reported that a short, evolutionarily conserved gene promoter regulates developmental expression of SI in mouse small intestine. Herein, we investigated the role of a hepatocyte nuclear factor-1 (HNF-1) cis-acting element to regulate SI gene expression in vivo. Transgenic SI gene constructs with a mutated HNF-1 element (SIF3) revealed a strong reduction in promoter activity in comparison with a wild-type construct in mice and during Caco-2 cell differentiation. Nuclear proteins isolated from enterocytes showed increased binding of the HNF-1alpha complex with a concomitant decrease in the HNF-1beta -containing complex to the SIF3 element both during the suckling-weaning developmental transition and Caco-2 cell differentiation. These changes coincided with a strong induction of SI gene transcription. In transfection experiments, HNF-1alpha activated the SI promoter via the SIF3 element, and co-expression of HNF-1beta impaired this transcriptional activation. These findings demonstrate the essential role of the HNF-1 regulatory element to support SI gene transcription in vivo and suggest that the ratio of HNF-1alpha to HNF-1beta plays a role in the transcriptional activity of this gene during intestinal development.

* The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.This

work was supported by RO1-DK46704 (to P.G.T.) and the Molecular Biology Core of the Center for Molecular Studies in Digestive Diseases at the University of Pennsylvania (P30-DK50306).

Dagger Supported by a postdoctoral fellowship from the "Fonds de la Recherche en Santé du Québec."

§ To whom correspondence should be addressed: 709 Swedeland Rd., P.O. Box 1539, King of Prussia, PA 19406-0939. Tel.: 610-270-6016; Fax: 610-270-6116; E-mail: Peter_G_Traber@sbphrd.com.

Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.


This article has been cited by other articles:


Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
C. R. Lussier, J.-P. Babeu, B. A. Auclair, N. Perreault, and F. Boudreau
Hepatocyte nuclear factor-4{alpha} promotes differentiation of intestinal epithelial cells in a coculture system
Am J Physiol Gastrointest Liver Physiol, February 1, 2008; 294(2): G418 - G428.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
T. Ezaki, R.-J. Guo, H. Li, A. B. Reynolds, and J. P. Lynch
The homeodomain transcription factors Cdx1 and Cdx2 induce E-cadherin adhesion activity by reducing beta- and p120-catenin tyrosine phosphorylation
Am J Physiol Gastrointest Liver Physiol, July 1, 2007; 293(1): G54 - G65.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
P. P. Singh, B. Voleti, and A. Agrawal
A Novel RBP-J{kappa}-Dependent Switch from C/EBPbeta to C/EBP{zeta} at the C/EBP Binding Site on the C-Reactive Protein Promoter
J. Immunol., June 1, 2007; 178(11): 7302 - 7309.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
T. Bosse, J. J. Fialkovich, C. M. Piaseckyj, E. Beuling, H. Broekman, R. J. Grand, R. K. Montgomery, and S. D. Krasinski
Gata4 and Hnf1{alpha} are partially required for the expression of specific intestinal genes during development
Am J Physiol Gastrointest Liver Physiol, May 1, 2007; 292(5): G1302 - G1314.
[Abstract] [Full Text] [PDF]

Home page
 J EndocrinolHome page
S. Kitanaka, U. Sato, and T. Igarashi
Regulation of human insulin, IGF-I, and multidrug resistance protein 2 promoter activity by hepatocyte nuclear factor (HNF)-1{beta} and HNF-1{alpha} and the abnormality of HNF-1{beta} mutants
J. Endocrinol., January 1, 2007; 192(1): 141 - 147.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pharmacol.Home page
L. Ng, K. Nichols, K. O'Rourke, A. Maslen, and G. M. Kirby
Repression of Human GSTA1 by Interleukin-1beta Is Mediated by Variant Hepatic Nuclear Factor-1C
Mol. Pharmacol., January 1, 2007; 71(1): 201 - 208.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Gautier-Stein, C. Zitoun, E. Lalli, G. Mithieux, and F. Rajas
Transcriptional Regulation of the Glucose-6-phosphatase Gene by cAMP/Vasoactive Intestinal Peptide in the Intestine: ROLE OF HNF4{alpha}, CREM, HNF1{alpha}, and C/EBP{alpha}
J. Biol. Chem., October 20, 2006; 281(42): 31268 - 31278.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
T. Bosse, H. M. van Wering, M. Gielen, L. N. Dowling, J. J. Fialkovich, C. M. Piaseckyj, F. J. Gonzalez, T. E. Akiyama, R. K. Montgomery, R. J. Grand, et al.
Hepatocyte nuclear factor-1{alpha} is required for expression but dispensable for histone acetylation of the lactase-phlorizin hydrolase gene in vivo
Am J Physiol Gastrointest Liver Physiol, May 1, 2006; 290(5): G1016 - G1024.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
B. Voleti and A. Agrawal
Regulation of Basal and Induced Expression of C-Reactive Protein through an Overlapping Element for OCT-1 and NF-{kappa}B on the Proximal Promoter
J. Immunol., September 1, 2005; 175(5): 3386 - 3390.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
J. Xu, M. Capezzone, X. Xu, and J. M. Hershman
Activation of Nicotinamide N-Methyltransferase Gene Promoter by Hepatocyte Nuclear Factor-1{beta} in Human Papillary Thyroid Cancer Cells
Mol. Endocrinol., February 1, 2005; 19(2): 527 - 539.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
L. Wang, A. Klopot, J.-N. Freund, L. N. Dowling, S. D. Krasinski, and J. C. Fleet
Control of differentiation-induced calbindin-D9k gene expression in Caco-2 cells by cdx-2 and HNF-1{alpha}
Am J Physiol Gastrointest Liver Physiol, November 1, 2004; 287(5): G943 - G953.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
J. K. Divine, L. J. Staloch, H. Haveri, C. M. Jacobsen, D. B. Wilson, M. Heikinheimo, and T. C. Simon
GATA-4, GATA-5, and GATA-6 activate the rat liver fatty acid binding protein gene in concert with HNF-1{alpha}
Am J Physiol Gastrointest Liver Physiol, November 1, 2004; 287(5): G1086 - G1099.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
H. M. van Wering, T. Bosse, A. Musters, E. de Jong, N. de Jong, C. E. Hogen Esch, F. Boudreau, G. P. Swain, L. N. Dowling, R. K. Montgomery, et al.
Complex regulation of the lactase-phlorizin hydrolase promoter by GATA-4
Am J Physiol Gastrointest Liver Physiol, October 1, 2004; 287(4): G899 - G909.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
L. Wang, C. Coffinier, M. K. Thomas, L. Gresh, G. Eddu, T. Manor, L. L. Levitsky, M. Yaniv, and D. B. Rhoads
Selective Deletion of the Hnf1{beta} (MODY5) Gene in {beta}-Cells Leads to Altered Gene Expression and Defective Insulin Release
Endocrinology, August 1, 2004; 145(8): 3941 - 3949.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
M. S. Keller, T. Ezaki, R.-J. Guo, and J. P. Lynch
Cdx1 or Cdx2 expression activates E-cadherin-mediated cell-cell adhesion and compaction in human COLO 205 cells
Am J Physiol Gastrointest Liver Physiol, July 1, 2004; 287(1): G104 - G114.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
S. Kitanaka, Y. Miki, Y. Hayashi, and T. Igarashi
Promoter-Specific Repression of Hepatocyte Nuclear Factor (HNF)-1{beta} and HNF-1{alpha} Transcriptional Activity by an HNF-1{beta} Missense Mutant Associated with Type 5 Maturity-Onset Diabetes of the Young with Hepatic and Biliary Manifestations
J. Clin. Endocrinol. Metab., March 1, 2004; 89(3): 1369 - 1378.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Clin. Nutr.Home page
W.-I. Leong, C. L Bowlus, J. Tallkvist, and B. Lonnerdal
Iron supplementation during infancy--effects on expression of iron transporters, iron absorption, and iron utilization in rat pups
Am. J. Clinical Nutrition, December 1, 2003; 78(6): 1203 - 1211.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Isshiki, T. Kudo, S. Nishihara, Y. Ikehara, A. Togayachi, A. Furuya, K. Shitara, T. Kubota, M. Watanabe, M. Kitajima, et al.
Lewis Type 1 Antigen Synthase ({beta}3Gal-T5) Is Transcriptionally Regulated by Homeoproteins
J. Biol. Chem., September 19, 2003; 278(38): 36611 - 36620.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
H. Schjerven, P. Brandtzaeg, and F.-E. Johansen
Hepatocyte NF-1 and STAT6 Cooperate with Additional DNA-Binding Factors to Activate Transcription of the Human Polymeric Ig Receptor Gene in Response to IL-4
J. Immunol., June 15, 2003; 170(12): 6048 - 6056.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
J. K. Divine, S. P. McCaul, and T. C. Simon
HNF-1{alpha} and endodermal transcription factors cooperatively activate Fabpl: MODY3 mutations abrogate cooperativity
Am J Physiol Gastrointest Liver Physiol, June 9, 2003; 285(1): G62 - G72.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Nauze, L. Gonin, B. Chaminade, C. Peres, F. Hullin-Matsuda, B. Perret, H. Chap, and A. Gassama-Diagne
Guinea Pig Phospholipase B, Identification of the Catalytic Serine and the Proregion Involved in Its Processing and Enzymatic Activity
J. Biol. Chem., November 8, 2002; 277(46): 44093 - 44099.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
D. Sauvaget, V. Chauffeton, D. Citadelle, F.-P. Chatelet, C. Cywiner-Golenzer, J. Chambaz, M. Pincon-Raymond, P. Cardot, J. Le Beyec, and A. Ribeiro
Restriction of Apolipoprotein A-IV Gene Expression to the Intestine Villus Depends on a Hormone-responsive Element and Parallels Differential Expression of the Hepatic Nuclear Factor 4alpha and gamma Isoforms
J. Biol. Chem., September 6, 2002; 277(37): 34540 - 34548.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
F. Boudreau, E. H. H. M. Rings, H. M. van Wering, R. K. Kim, G. P. Swain, S. D. Krasinski, J. Moffett, R. J. Grand, E. R. Suh, and P. G. Traber
Hepatocyte Nuclear Factor-1alpha , GATA-4, and Caudal Related Homeodomain Protein Cdx2 Interact Functionally to Modulate Intestinal Gene Transcription. IMPLICATION FOR THE DEVELOPMENTAL REGULATION OF THE SUCRASE-ISOMALTASE GENE
J. Biol. Chem., August 23, 2002; 277(35): 31909 - 31917.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
F. Boudreau, E. H. H. M. Rings, G. P. Swain, A. M. Sinclair, E. R. Suh, D. G. Silberg, R. H. Scheuermann, and P. G. Traber
A Novel Colonic Repressor Element Regulates Intestinal Gene Expression by Interacting with Cux/CDP
Mol. Cell. Biol., August 1, 2002; 22(15): 5467 - 5478.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. Sakaguchi, X. Gu, H. M. Golden, E. Suh, D. B. Rhoads, and H.-C. Reinecker
Cloning of the Human Claudin-2 5'-Flanking Region Revealed a TATA-less Promoter with Conserved Binding Sites in Mouse and Human for Caudal-related Homeodomain Proteins and Hepatocyte Nuclear Factor-1alpha
J. Biol. Chem., June 7, 2002; 277(24): 21361 - 21370.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2001 by the American Society for Biochemistry and Molecular Biology.