HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 276, Issue 34, 32274-32281, August 24, 2001

This Article Full Text Full Text (PDF) All Versions of this Article: 276/34/32274    most recent M103285200v1 Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Fotinou, C. Articles by Isaacs, N. W. Search for Related Content PubMed PubMed Citation Articles by Fotinou, C. Articles by Isaacs, N. W. Social Bookmarking                      What's this?

The Crystal Structure of Tetanus Toxin Hc Fragment Complexed with a Synthetic GT1b Analogue Suggests Cross-linking between Ganglioside Receptors and the Toxin^*

Constantina Fotinou, Paul Emsley, Isobel Black, Hiromune Ando^§, Hideharu Ishida^§, Makoto Kiso^§, Katharine A. Sinha^¶, Neil F. Fairweather^¶, and Neil W. Isaacs

From the  Department of Chemistry, University of Glasgow, Glasgow, G12 8QQ, Scotland, the ^§ Department of Applied Bio-organic Chemistry, Gifu University, Gifu 501-1193, Japan, and the ^¶ Department of Biochemistry, Imperial College of Science and Technology Medicine, London SW7 2AZ, United Kingdom

Tetanus toxin, a member of the family of Clostridial neurotoxins, is one of the most potent toxins known. The crystal structure of the complex of the COOH-terminal fragment of the heavy chain with an analogue of its ganglioside receptor, GT1b, provides the first direct identification and characterization of the ganglioside-binding sites. The ganglioside induces cross-linking by binding to two distinct sites on the Hc molecule. The structure sheds new light on the binding of Clostridial neurotoxins to receptors on neuronal cells and provides important information relevant to the design of anti-tetanus and anti-botulism therapeutic agents.

The atomic coordinates and the structure factors (code 1fv2 and 1fv3) have been deposited in the Protein Data Bank, Research Collaboratory for Structural Bioinformatics, Rutgers University, New Brunswick, NJ (http://www.rcsb.org/).

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				A. Fischer, C. Garcia-Rodriguez, I. Geren, J. Lou, J. D. Marks, T. Nakagawa, and M. Montal Molecular Architecture of Botulinum Neurotoxin E Revealed by Single Particle Electron Microscopy J. Biol. Chem., February 15, 2008; 283(7): 3997 - 4003. [Abstract] [Full Text] [PDF]

				A. Rummel, T. Eichner, T. Weil, T. Karnath, A. Gutcaits, S. Mahrhold, K. Sandhoff, R. L. Proia, K. R. Acharya, H. Bigalke, et al. Identification of the protein receptor binding site of botulinum neurotoxins B and G proves the double-receptor concept PNAS, January 2, 2007; 104(1): 359 - 364. [Abstract] [Full Text] [PDF]

				H. Attrill, A. Imamura, R. S. Sharma, M. Kiso, P. R. Crocker, and D. M. F. van Aalten Siglec-7 Undergoes a Major Conformational Change When Complexed with the {alpha}(2,8)-Disialylganglioside GT1b J. Biol. Chem., October 27, 2006; 281(43): 32774 - 32783. [Abstract] [Full Text] [PDF]

				O. Qazi, D. Sesardic, R. Tierney, Z. Soderback, D. Crane, B. Bolgiano, and N. Fairweather Reduction of the Ganglioside Binding Activity of the Tetanus Toxin HC Fragment Destroys Immunogenicity: Implications for Development of Novel Tetanus Vaccines. Infect. Immun., August 1, 2006; 74(8): 4884 - 4891. [Abstract] [Full Text] [PDF]

				J. Moore, S. E. S. Bailey, Z. Benmechernene, C. Tzitzilonis, N. J. E. Griffiths, M. Virji, and J. P. Derrick Recognition of Saccharides by the OpcA, OpaD, and OpaB Outer Membrane Proteins from Neisseria meningitidis J. Biol. Chem., September 9, 2005; 280(36): 31489 - 31497. [Abstract] [Full Text] [PDF]

				C. X. Moss, S. P. Matthews, D. J. Lamont, and C. Watts Asparagine Deamidation Perturbs Antigen Presentation on Class II Major Histocompatibility Complex Molecules J. Biol. Chem., May 6, 2005; 280(18): 18498 - 18503. [Abstract] [Full Text] [PDF]

				A. Rummel, T. Karnath, T. Henke, H. Bigalke, and T. Binz Synaptotagmins I and II Act as Nerve Cell Receptors for Botulinum Neurotoxin G J. Biol. Chem., July 16, 2004; 279(29): 30865 - 30870. [Abstract] [Full Text] [PDF]

				M. S. Alphey, H. Attrill, P. R. Crocker, and D. M. F. van Aalten High Resolution Crystal Structures of Siglec-7. INSIGHTS INTO LIGAND SPECIFICITY IN THE SIGLEC FAMILY J. Biol. Chem., January 24, 2003; 278(5): 3372 - 3377. [Abstract] [Full Text] [PDF]

				B. C. Yowler, R. D. Kensinger, and C.-L. Schengrund Botulinum Neurotoxin A Activity Is Dependent upon the Presence of Specific Gangliosides in Neuroblastoma Cells Expressing Synaptotagmin I J. Biol. Chem., August 30, 2002; 277(36): 32815 - 32819. [Abstract] [Full Text] [PDF]

				A. Nowakowski, C. Wang, D. B. Powers, P. Amersdorfer, T. J. Smith, V. A. Montgomery, R. Sheridan, R. Blake, L. A. Smith, and J. D. Marks Potent neutralization of botulinum neurotoxin by recombinant oligoclonal antibody PNAS, August 20, 2002; 99(17): 11346 - 11350. [Abstract] [Full Text] [PDF]